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Molecular mapping of the genomic region conferring resistance to soybean stem canker in Hutcheson soybean

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Abstract

Genetic resistance to soybean stem canker, caused by the fungus Diaporthe phaseolorum var. meridionalis (Dpm), is controlled by five major, dominant, nonallelic genes Rdm1 to Rdm5. A genomic region containing the Rdm4 and Rdm5 genes was first described in Hutcheson soybean, where they were found to confer specific resistance to Argentinean physiological races of Dpm. Here, we report the genetic mapping of Rdm4 and Rdm5 loci using two pheno- and genotypically characterized F2:3 populations derived from Hutcheson cultivar. The mapping populations were screened with amplified fragment length polymorphism (AFLP) markers using bulk segregant analysis, and with simple sequence repeat (SSR) markers. Linkage analysis indicated that the Rdm4 and Rdm5 resistance loci were located in a genomic region collinear with the molecular linkage group (MLG) A2 (chromosome 8) of the soybean genetic map. The linkage group contains two SSR markers, Sat_162 and Satt233, flanking the Rdm4 and Rdm5 loci. These SSR will be useful to increase the efficiency of selection in breeding programs aimed to incorporate Rdm4 and Rdm5 genes into soybean elite germplasm.

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Acknowledgments

This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) PID 22995-04 BID 1728/OC-AR and PICT N° 08-09890, to ENM. MAC and ENM are Career Investigators of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Soybean experimental line J77-339 used in this work was gently provided by Dr. Thomas C. Kilen.

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Authors and Affiliations

  1. Laboratorio de Fisiología Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, P.O. Box 14, 2125, Santa Fe, Argentina

    M. A. Chiesa, M. V. Cambursano & E. N. Morandi

  2. Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR, CONICET-UNR), Santa Fe, Argentina

    M. A. Chiesa, R. N. Pioli & E. N. Morandi

  3. Laboratorio de Biodiversidad Vegetal y Microbiana y Fitopatología, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Santa Fe, Argentina

    R. N. Pioli

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  1. M. A. Chiesa
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  2. M. V. Cambursano
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  3. R. N. Pioli
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  4. E. N. Morandi
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Contributions

M.A.C. and E.N.M. designed the research; M.A.C., M.V.C., R.N.P. and E.N.M. performed the research; M.A.C and R.N.P. analysed the plant-pathogen interaction data; M.A.C. and M.V.C. analysed the molecular data; and M.A.C. and E.N.M wrote the manuscript.

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Correspondence to E. N. Morandi.

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Chiesa, M.A., Cambursano, M.V., Pioli, R.N. et al. Molecular mapping of the genomic region conferring resistance to soybean stem canker in Hutcheson soybean. Mol Breeding 37, 65 (2017). https://doi.org/10.1007/s11032-017-0660-6

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