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Mapping QTLs of resistance to head splitting in cabbage (Brassica oleracea L.var. capitata L.)

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Abstract

Cabbage head splitting can greatly affect both the quality and commercial value of cabbage (Brassica oleracea). To detect the genetic basis of head-splitting resistance, a genetic map was constructed using an F2 population derived by crossing “748” (head-splitting-resistant inbred line) and “747” (head-splitting-susceptible inbred line). The map spans 830.9 cM and comprises 270 markers distributed in nine linkage groups, which correspond to the nine chromosomes of B. oleracea. The average distance between adjacent markers was 3.6 cM. A total of six quantitative trait loci (QTLs) conferring resistance to head splitting were detected in chromosome 2, 4, and 6. Two QTLs, SPL-2-1 and SPL-4-1, on chromosomes 2 and 4, respectively, were detected in the experiments over 2 years, suggesting that these two potential loci were important for governing the head-splitting resistance trait. Markers BRPGM0676 and BRMS137, which were tightly linked with head-splitting resistance, were detected in the conserved QTL SPL-2-1 region using bulked segregant analysis. Synteny analysis showed that SPL-2-1 was anchored to a 3.18-Mb genomic region of the B. oleracea genome, homologous to crucifer ancestral karyotype E block in chromosome 1 of Arabidopsis thaliana. Moreover, using a field emission scanning electron microscope, significant differences were observed between the two parental lines in terms of cell structures. Line “747” had thinner cell wall, lower cell density, larger cell size, and anomalous cell wall structure compared with the resistant line “748.” The different cell structures can provide a cytological base for assessing cabbage head splitting.

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Acknowledgments

This work was supported by the Golden Seed Projet (No. 213002-04-1-SB110), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA), and Korea Forest Service (KFS).

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    Authors and Affiliations

    1. Molecular Genetics and Genomics Lab, Department of Horticulture, Chungnam National University, Daejeon, 305-764, Republic of Korea

      Wenxing Pang, Xiaonan Li, Su Ryun Choi, Van Dan Nguyen, Vignesh Dhandapani, Yoon-Young Kim, Jin Gyu Kim & Yong Pyo Lim

    2. Translational and Evolutionary Genomics Lab, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

      Nirala Ramchiary

    3. School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, 4072, Australia

      David Edwards

    4. School of Plant Biology, University of Western Australia, Crawley, WA, 6009, Australia

      Jacqueline Batley

    5. Research and Development, Nunhems Korea, Anseong, 456-883, Republic of Korea

      Jonghyun Na

    6. Plant Systems Engineering Research Center, Cabbage Genomics Assisted Breeding Supporting Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-806, Republic of Korea

      HyeRan Kim

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    1. Wenxing Pang
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    Correspondence to Yong Pyo Lim.

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    Wenxing Pang and Xiaonan Li have contributed equally to this work.

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    Fig. S1

    Continuous distribution of head-splitting severity displayed in the F2:3 segregating population in year 2012 (A) and 2014 (B) experiment, respectively. (PPTX 72 kb)

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    Pang, W., Li, X., Choi, S.R. et al. Mapping QTLs of resistance to head splitting in cabbage (Brassica oleracea L.var. capitata L.). Mol Breeding 35, 126 (2015). https://doi.org/10.1007/s11032-015-0318-1

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