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Natural variation of TaGASR7-A1 affects grain length in common wheat under multiple cultivation conditions

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Abstract

GASR7 is a member of Snakin/GASA gene family in higher plants and has been found associated with grain length (GL) in rice and wheat under normal growth conditions. Here, we report the characterization of three distinct TaGASR7 homoeologs (TaGASR7-A1, TaGASR7-B1 and TaGASR7-D1) in common wheat and their deduced proteins and haplotype variation. TaGASR7 homoeologs were located on wheat group 7 chromosomes. Compared with previously characterized Snakin/GASA members, the central region in deduced TaGASR7 proteins and their orthologs was unique in containing a polyglycine tract. Through analyzing longer genomic sequence, more nucleotide differences were found for the two previously reported major haplotypes (H1c and H1g) of TaGASR7-A1. In contrast, no haplotype variation was detected for TaGASR7-B1 and TaGASR7-D1 in the 94 elite common wheat varieties examined. H1c, but not H1g, tended to associate with larger GL values in nine cultivation environments differing in water and nutrient application. However, the positive association between H1c and other grain traits (grain weight and yield) was affected by cultivation environment. Both H1c- and H1g-type alleles were more highly expressed in the unfertilized caryopses and those collected at 5 days after flowering (DAF). Interestingly, at 5 DAF, the expression level of H1c-type alleles was significantly lower than that of H1g-type alleles. By combining our data with those published previously, we suggest that TaGASR7-A1 is mainly a genetic determinant of GL in wheat with pleiotropic effects on grain weight and yield. Potential mechanism underlying TaGASR7-A1 function and its utility in enhancing genetic and breeding studies of wheat grain morphometric and yield traits are discussed.

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References

  • Bai X, Wu B, Xing Y (2012) Yield-related QTLs and their applications in rice genetic improvement. J Integr Plant Biol 54:300–311

    Article  PubMed  Google Scholar 

  • Berrocal-Lobo M, Segura A, Moreno M, López G, García-Olmedo F, Molina A (2002) Snakin-2, an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection. Plant Physiol 128:951–961

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Breseghello F, Sorrells ME (2006) Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics 172:1165–1177

    Article  PubMed  PubMed Central  Google Scholar 

  • Campbell KJ, Bergman CJ, Gualberto DG, Anderson JA, Giroux MJ, Hareland G, Fulcher RG, Sorrells ME, Finney PL (1999) Quantitative trait loci associated with kernel traits in a soft × hard wheat cross. Crop Sci 39:1184–1195

    Article  CAS  Google Scholar 

  • Cui F, Ding AM, Li J, Zhao CH, Li XF, Feng DS, Wang XQ, Wang L, Gao JR, Wang HG (2011) Wheat kernel dimensions: how do they contribute to kernel weight at an individual QTL level? J Genet 90:409–425

    Article  PubMed  Google Scholar 

  • Devos KM, Gale MD (2000) Genome relationships: the grass model in current research. Plant Cell 12:637–646

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Dholakia BB, Ammiraju JSS, Singh H, Lagu MD, Röder MS, Rao VS, Dhaliwal HS, Ranjekar PK, Gupta VS (2003) Molecular marker analysis of kernel size and shape in bread wheat. Plant Breed 122:392–395

    Article  CAS  Google Scholar 

  • Dong L, Zhang X, Liu D, Fan H, Sun J, Zhang Z, Qin H, Li B, Hao S, Li Z, Wang D, Zhang A, Ling HQ (2010) New insights into the organization, recombination, expression and functional mechanism of low molecular weight glutenin subunit genes in bread wheat. PLoS One 5:e13548

    Article  PubMed  PubMed Central  Google Scholar 

  • Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res 8:186–194

    Article  PubMed  CAS  Google Scholar 

  • Ewing B, Hillier L, Wendl MC, Green P (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res 8:175–185

    Article  PubMed  CAS  Google Scholar 

  • Fan C, Xing Y, Mao H, Lu T, Han B, Xu C, Li X, Zhang Q (2006) GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor Appl Genet 112:1164–1171

    Article  PubMed  CAS  Google Scholar 

  • Fan C, Yu S, Wang C, Xing Y (2009) A causal C-A mutation in the second exon of GS3 highly associated with rice grain length and validated as a functional marker. Theor Appl Genet 118:465–472

    Article  PubMed  CAS  Google Scholar 

  • Feng B, Dong Z, Xu Z, An X, Qin H, Wu N, Wang D, Wang T (2010) Molecular analysis of lipoxygenase (LOX) genes in common wheat and phylogenetic investigation of LOX proteins from model and crop plants. J Cereal Sci 52:387–394

    Article  CAS  Google Scholar 

  • Gegas VC, Nazari A, Griffiths S, Simmonds J, Fish L, Orford S, Sayers L, Doonan JH, Snape JW (2010) A genetic framework for grain size and shape variation in wheat. Plant Cell 22:1046–1056

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Huang X, Zhao Y, Wei X, Li C, Wang A, Zhao Q, Li W, Guo Y, Deng L, Zhu C, Fan D, Lu Y, Weng Q, Liu K, Zhou T, Jing Y, Si L, Dong G, Huang T, Lu T, Feng Q, Qian Q, Li J, Han B (2012) Genome-wide association study of flowering time and grain yield traits in a worldwide collection of rice germplasm. Nat Genet 44:32–39

    Article  Google Scholar 

  • Huang R, Jiang L, Zheng J, Wang T, Wang H, Huang Y, Hong Z (2013) Genetic bases of rice grain shape: so many genes, so little known. Trends Plant Sci 18:218–226

    Article  PubMed  CAS  Google Scholar 

  • Ling HQ, Zhao S, Liu D, Wang J, Sun H, Zhang C, Fan H, Li D, Dong L, Tao Y, Gao C, Wu H, Li Y, Cui Y, Guo X, Zheng S, Wang B, Yu K, Liang Q, Yang W, Lou X, Chen J, Feng M, Jian J, Zhang X, Luo G, Jiang Y, Liu J, Wang Z, Sha Y, Zhang B, Wu H, Tang D, Shen Q, Xue P, Zou S, Wang X, Liu X, Wang F, Yang Y, An X, Dong Z, Zhang K, Zhang X, Luo MC, Dvorak J, Tong Y, Wang J, Yang H, Li Z, Wang D, Zhang A, Wang J (2013) Draft genome of the wheat A-genome progenitor Triticum urartu. Nature 496:87–90

    Article  PubMed  CAS  Google Scholar 

  • Liu RH, Meng JL (2003) MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Heraditas (Beijing) 25:317–321

    Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the \(2^{{- \varDelta \varDelta {\text{C}}_{\text{T}}}}\) method. Methods 25:402–408

  • Mangeon A, Junqueira RM, Sachetto-Martins G (2010) Functional diversity of the plant glycine-rich proteins superfamily. Plant Signal Behav 5:99–104

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Park AR, Cho SK, Yun UJ, Jin MY, Lee SH, Sachetto-Martins G, Park OK (2001) Interaction of the Arabidopsis receptor protein kinase Wak1 with a glycine-rich protein, AtGRP-3. J Biol Chem 276:26688–26693

    Article  PubMed  CAS  Google Scholar 

  • Porto WF, Franco OL (2013) Theoretical structural insights into the Snakin/GASA family. Peptides 44:163–167

    Article  PubMed  CAS  Google Scholar 

  • Qi L, Echalier B, Friebe B, Gill BS (2003) Molecular characterization of a set of wheat deletion stocks for use in chromosome bin mapping of ESTs. Funct Integr Genomics 3:39–55

    PubMed  CAS  Google Scholar 

  • Quarrie SA, Quarrie SP, Radosevic R, Rancic D, Kaminska A, Barnes JD, Leverington M, Ceoloni C, Dodig D (2006) Dissecting a wheat QTL for yield present in a range of environments: from the QTL to candidate genes. J Exp Bot 57:2627–2637

    Article  PubMed  CAS  Google Scholar 

  • Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C, Zhang J, Fulton L, Graves TA, Minx P, Reily AD, Courtney L, Kruchowski SS, Tomlinson C, Strong C, Delehaunty K, Fronick C, Courtney B, Rock SM, Belter E, Du F, Kim K, Abbott RM, Cotton M, Levy A, Marchetto P, Ochoa K, Jackson SM, Gillam B, Chen W, Yan L, Higginbotham J, Cardenas M, Waligorski J, Applebaum E, Phelps L, Falcone J, Kanchi K, Thane T, Scimone A, Thane N, Henke J, Wang T, Ruppert J, Shah N, Rotter K, Hodges J, Ingenthron E, Cordes M, Kohlberg S, Sgro J, Delgado B, Mead K, Chinwalla A, Leonard S, Crouse K, Collura K, Kudrna D, Currie J, He R, Angelova A, Rajasekar S, Mueller T, Lomeli R, Scara G, Ko A, Delaney K, Wissotski M, Lopez G, Campos D, Braidotti M, Ashley E, Golser W, Kim H, Lee S, Lin J, Dujmic Z, Kim W, Talag J, Zuccolo A, Fan C, Sebastian A, Kramer M, Spiegel L, Nascimento L, Zutavern T, Miller B, Ambroise C, Muller S, Spooner W, Narechania A, Ren L, Wei S, Kumari S, Faga B, Levy MJ, McMahan L, Van Buren P, Vaughn MW, Ying K, Yeh CT, Emrich SJ, Jia Y, Kalyanaraman A, Hsia AP, Barbazuk WB, Baucom RS, Brutnell TP, Carpita NC, Chaparro C, Chia JM, Deragon JM, Estill JC, Fu Y, Jeddeloh JA, Han Y, Lee H, Li P, Lisch DR, Liu S, Liu Z, Nagel DH, McCann MC, SanMiguel P, Myers AM, Nettleton D, Nguyen J, Penning BW, Ponnala L, Schneider KL, Schwartz DC, Sharma A, Soderlund C, Springer NM, Sun Q, Wang H, Waterman M, Westerman R, Wolfgruber TK, Yang L, Yu Y, Zhang L, Zhou S, Zhu Q, Bennetzen JL, Dawe RK, Jiang J, Jiang N, Presting GG, Wessler SR, Aluru S, Martienssen RA, Clifton SW, McCombie WR, Wing RA, Wilson RK (2009) The B73 maize genome: complexity, diversity, and dynamics. Science 326:1112–1115

    Article  PubMed  CAS  Google Scholar 

  • Sears ER (1954) The aneuploids of common wheat. Mo Agric Exp Stn Res Bull 572:1–59

    Google Scholar 

  • Segura A, Moreno M, Madueño F, Molina A, García-Olmedo F (1999) Snakin-1, a peptide from potato that is active against plant pathogens. Mol Plant Microbe Interact 12:16–23

    Article  PubMed  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Song XJ, Huang W, Shi M, Zhu MZ, Lin HX (2007) A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nat Genet 39:623–630

    Article  PubMed  CAS  Google Scholar 

  • Sorrells ME, La Rota M, Bermudez-Kandianis CE, Greene RA, Kantety R, Munkvold JD, Miftahudin MA, Ma X, Gustafson PJ, Qi LL, Echalier B, Gill BS, Matthews DE, Lazo GR, Chao S, Anderson OD, Edwards H, Linkiewicz AM, Dubcovsky J, Akhunov ED, Dvorak J, Zhang D, Nguyen HT, Peng J, Lapitan NL, Gonzalez-Hernandez JL, Anderson JA, Hossain K, Kalavacharla V, Kianian SF, Choi DW, Close TJ, Dilbirligi M, Gill KS, Steber C, Walker-Simmons MK, McGuire PE, Qualset CO (2003) Comparative DNA sequence analysis of wheat and rice genomes. Genome Res 13:1818–1827

    PubMed  CAS  PubMed Central  Google Scholar 

  • Su Z, Hao C, Wang L, Dong Y, Zhang X (2011) Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.). Theor Appl Genet 122:211–223

    Article  PubMed  CAS  Google Scholar 

  • Sun XY, Wu K, Zhao Y, Kong FM, Han GZ, Jiang HM, Huang XJ, Li RJ, Wang HG, Li SS (2009) QTL analysis of kernel shape and weight using recombinant inbred lines in wheat. Euphytica 165:615–624

    Article  CAS  Google Scholar 

  • Takano-Kai N, Jiang H, Kubo T, Sweeney M, Matsumoto T, Kanamori H, Padhukasahasram B, Bustamante C, Yoshimura A, Doi K, McCouch S (2009) Evolutionary history of GS3, a gene conferring grain length in rice. Genetics 182:1323–1334

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Tsilo T, Hareland GA, Simsek S, Chao SM, Anderson JA (2010) Genome mapping of kernel characteristics in hard red spring wheat breeding lines. Theor Appl Genet 121:717–730

    Article  PubMed  CAS  Google Scholar 

  • Vogel JP, Garvin DF, Mockler TC, Schmutz J, Rokhsar D, Bevan MW, Barry K, Lucas S, Harmon-Smith M, Lail K, Tice H, Schmutz J, Grimwood J, McKenzie N, Bevan MW, Huo N, Gu YQ, Lazo GR, Anderson OD, Vogel JP, You FM, Luo MC, Dvorak J, Wright J, Febrer M, Bevan MW, Idziak D, Hasterok R, Garvin DF, Lindquist E, Wang M, Fox SE, Priest HD, Filichkin SA, Givan SA, Bryant DW, Chang JH, Mockler TC, Wu H, Wu W, Hsia AP, Schnable PS, Kalyanaraman A, Barbazuk B, Michael TP, Hazen SP, Bragg JN, Laudencia-Chingcuanco D, Vogel JP, Garvin DF, Weng Y, McKenzie N, Bevan MW, Haberer G, Spannagl M, Mayer K, Rattei T, Mitros T, Rokhsar D, Lee SJ, Rose JK, Mueller LA, York TL, Wicker T, Buchmann JP, Tanskanen J, Schulman AH, Gundlach H, Wright J, Bevan M, de Oliveira AC, Maia Lda C, Belknap W, Gu YQ, Jiang N, Lai J, Zhu L, Ma J, Sun C, Pritham E, Salse J, Murat F, Abrouk M, Haberer G, Spannagl M, Mayer K, Bruggmann R, Messing J, You FM, Luo MC, Dvorak J, Fahlgren N, Fox SE, Sullivan CM, Mockler TC, Carrington JC, Chapman EJ, May GD, Zhai J, Ganssmann M, Gurazada SG, German M, Meyers BC, Green PJ, Bragg JN, Tyler L, Wu J, Gu YQ, Lazo GR, Laudencia-Chingcuanco D, Thomson J, Vogel JP, Hazen SP, Chen S, Scheller HV, Harholt J, Ulvskov P, Fox SE, Filichkin SA, Fahlgren N, Kimbrel JA, Chang JH, Sullivan CM, Chapman EJ, Carrington JC, Mockler TC, Bartley LE, Cao P, Jung KH, Sharma MK, Vega-Sanchez M, Ronald P, Dardick CD, De Bodt S, Verelst W, Inzé D, Heese M, Schnittger A, Yang X, Kalluri UC, Tuskan GA, Hua Z, Vierstra RD, Garvin DF, Cui Y, Ouyang S, Sun Q, Liu Z, Yilmaz A, Grotewold E, Sibout R, Hematy K, Mouille G, Höfte H, Michael T, Pelloux J, O’Connor D, Schnable J, Rowe S, Harmon F, Cass CL, Sedbrook JC, Byrne ME, Walsh S, Higgins J, Bevan M, Li P, Brutnell T, Unver T, Budak H, Belcram H, Charles M, Chalhoub B, Baxter I (2010) Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature 463:763–768

    Article  CAS  Google Scholar 

  • Wan Y, Wang D, Shewry R, Halford G (2002) Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet 104:828–839

    Article  PubMed  CAS  Google Scholar 

  • Wang S, Wu K, Yuan Q, Liu X, Liu Z, Lin X, Zeng R, Zhu H, Dong G, Qian Q, Zhang G, Fu X (2012) Control of grain size, shape and quality by OsSPL16 in rice. Nat Genet 44:950–954

    Article  PubMed  CAS  Google Scholar 

  • Wilkinson PA, Winfield MO, Barker GL, Allen AM, Burridge A, Coghill JA, Edwards KJ (2012) CerealsDB 2.0: an integrated resource for plant breeders and scientists. BMC Bioinform 13:219

    Article  Google Scholar 

  • Wu X, Chang X, Jing R (2012) Genetic insight into yield-associated traits of wheat grown in multiple rain-fed environments. PLoS One 7:e31249

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Xing Y, Zhang Q (2010) Genetic and molecular bases of rice yield. Annu Rev Plant Biol 61:421–422

    Article  PubMed  CAS  Google Scholar 

  • Yang Z, Bai Z, Li X, Wang P, Wu Q, Yang L, Li L, Li X (2012) SNP identification and allelic-specific PCR markers development for TaGW2, a gene linked to wheat kernel weight. Theor Appl Genet 125:1057–1068

    Article  PubMed  CAS  Google Scholar 

  • Zhang X, Wang J, Huang J, Lan H, Wang C, Yin C, Wu Y, Tang H, Qian Q, Li J, Zhang H (2012) Rare allele of OsPPKL1 associated with grain length causes extra-large grain and significant yield increase in rice. Proc Natl Acad Sci USA 109:21534–21539

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Zhang K, Wang J, Zhang L, Rong C, Zhao F, Peng T, Li H, Cheng D, Liu X, Qin H, Zhang A, Tong Y, Wang D (2013) Association analysis of genomic loci important for grain weight control in elite common wheat varieties cultivated with variable water and fertiliser supply. PLoS One 8:e57853

    Article  PubMed  CAS  PubMed Central  Google Scholar 

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Acknowledgments

This work was supported by the Ministry of Science and Technology of China (Grants 2012AA10A308 and 2011BAD07B02-2 and the special fund for the State Key Laboratory of Plant Cell and Chromosome Engineering). We thank Ms Shuangjuan Yang for technical assistance in collecting caryopsis samples.

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Authors and Affiliations

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Lingli Dong, Fangming Wang, Tao Liu, Zhenying Dong, Yiwen Li, Xin Liu, Kunpu Zhang & Daowen Wang

  2. Graduate University of Chinese Academy of Sciences, Yuquan Road, Beijing, 100039, China

    Fangming Wang

  3. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

    Aili Li, Ruilian Jing & Long Mao

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  1. Lingli Dong
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  2. Fangming Wang
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  3. Tao Liu
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Correspondence to Kunpu Zhang or Daowen Wang.

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Dong, L., Wang, F., Liu, T. et al. Natural variation of TaGASR7-A1 affects grain length in common wheat under multiple cultivation conditions. Mol Breeding 34, 937–947 (2014). https://doi.org/10.1007/s11032-014-0087-2

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