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Genetic characterisation and fine mapping of a chlorophyll-deficient mutant (BnaC.ygl) in Brassica napus

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Abstract

A chlorophyll-deficient mutant with yellow-green leaves of Brassica napus was obtained by treatment with the chemical mutagen ethyl methanesulfonate. Compared with the wild type at seedling stage, the mutant displayed decreased total chlorophyll content, less granal stacks and granal membranes. Genetic analysis confirmed that the mutant phenotype was controlled by a recessive gene, which was designated as BnaC.ygl. Mapping of the gene was subsequently conducted in two populations with yellow-green leaves (population IBC8 and IIBC4, which comprised 3,472 and 5,288 individuals, respectively). Analysis on the public simple sequence repeat markers (SSR) showed that four SSR markers linked to BnaC.YGL gene displayed polymorphism. Based on the information of these SSR markers, the BnaC.YGL gene was mapped to the linkage group N17. From a survey of amplified fragment length polymorphism (AFLP), 15 of 47 AFLP markers were successfully converted into sequence characterised amplified region (SCAR) markers. BnY5 and CB10534, the closest flanking markers, were 0.32 and 0.03 cM away from the BnaC.YGL gene, respectively. And in the two populations, 18 makers cosegregated with BnaC.YGL. BLAST analysis revealed that the sequences of the makers displayed highly conserved homology with C06 of B. oleracea. The collinearity of makers to makers on N17 and on C06 showed that there might be an inversion occurring on the N17 group. These results are expected to accelerate the process of cloning the BnaC.YGL gene and facilitate the understanding of the biological processes of chloroplast development in Brassica napus.

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Acknowledgments

We sincerely thank Dr. Shengyi Liu (Oilcrops Research Institute, Chinese Academy of Agricultural Sciences) for providing the scaffold sequences of B. oleracea. This research was financed by funds from the Program for Modern Agricultural Industrial Technology System (nycytx-00501), the Nature High-tech R & D Program “863’’ (2012AA101107, 2011AA10A104), the National Science & Technology Support Program (2012BAD49G00, 2011BAD35B04).

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  1. National Key Laboratory of Crop Genetic Improvement, National Sub-center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, 430070, China

    Lixia Zhu, Xinhua Zeng, Yanli Chen, Zonghui Yang, Liping Qi, Yuanyuan Pu, Bin Yi, Jing Wen, Chaozhi Ma, Jinxiong Shen, Jinxing Tu & Tingdong Fu

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  1. Lixia Zhu
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Zhu, L., Zeng, X., Chen, Y. et al. Genetic characterisation and fine mapping of a chlorophyll-deficient mutant (BnaC.ygl) in Brassica napus . Mol Breeding 34, 603–614 (2014). https://doi.org/10.1007/s11032-014-0060-0

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