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TBP-assisted species and hybrid identification in the genus Passiflora

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Abstract

We used the tubulin-based-polymorphism (TBP) method to analyze 45 different species and interspecific hybrids of the genus Passiflora. With regard to genetic characterization, the TBP method is a convenient, fast, simple and reliable tool for assigning a specific polymorphic pattern (i.e., molecular fingerprint) to each of the species and hybrids analyzed. In the case of hybrids, an accurate, easy and immediate recognition of parental contribution is an additional benefit of the TBP method. Molecular data collected using the TBP marker system resulted in a classification of the species of Passiflora that is fully consistent with previous studies performed with a variety of nuclear and chloroplast markers. Similar to those molecular studies, the TBP phylogenetic classification of the various species differs from that obtained using the more restricted number of morphological markers. Overall, this outcome indicates the limited value of these descriptors with respect to genomic diversity when discriminating among different species of Passiflora.

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Acknowledgments

We are very grateful to the breeder Maurizio Vecchia for hybrid donations and we thank Marco Ballardini and Fulvio Dente for their help in laboratory work. This work was partially supported by the Ministry of Agricultural, Food and Forestry Policies, Italy, Project RGV-FAO.

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Authors and Affiliations

  1. CNR, Institute of Agricultural Biology and Biotechnology (IBBA), Via Edoardo Bassini, 15, 20133, Milan, Italy

    Luca Braglia, Floriana Gavazzi & Diego Breviario

  2. Consiglio Per la Ricerca e la Sperimentazione in Agricoltura, Unità di Ricerca Per la Floricoltura e le Specie Ornamentali (CRA-FSO), Corso Inglesi, 508, 18038, Sanremo, Imperia, Italy

    Annalisa Giovannini, Federica Nicoletti & Laura De Benedetti

Authors
  1. Luca Braglia
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  2. Floriana Gavazzi
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  3. Annalisa Giovannini
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  4. Federica Nicoletti
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  5. Laura De Benedetti
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  6. Diego Breviario
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Corresponding author

Correspondence to Diego Breviario.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Online Resource 1

Dataset of Passiflora samples used for morphological analysis. See Table 1 for OTU codes and Table 2 for character list (DOCX 20 kb)

Online Resource 2

Pictures explaining some of the selected Passiflora morphological characters listed in Table 2 (JPG 1999 kb)

Online Resource 3

Principal Component Analysis. Percentage and cumulative variances and eigen-vectors on the first four components for each character in 41 Passiflora morphotypes (DOCX 14 kb)

Online Resource 4

Polyacrylamide gel electrophoresis of the TBP amplification profile for β-tubulin intron I in different Passiflora genotypes. Sample codes are described in Table 1. Marker sizes were estimated by comparison to molecular mass standards indicated at both sides (JPG 1268 kb)

Online Resource 5

Summary of the numeric data output provided by AB 310 Genetic Analyzer system. Each hybrid is placed in a row between the respective parents, that are indicated in bold character. S stays for sizes, expressed in nucleotide base pairs and R refers to the Relative Fluorescence Unit for the signal intensity of the corresponding peak (DOCX 24 kb)

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Braglia, L., Gavazzi, F., Giovannini, A. et al. TBP-assisted species and hybrid identification in the genus Passiflora . Mol Breeding 33, 209–219 (2014). https://doi.org/10.1007/s11032-013-9945-6

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  • DOI: https://doi.org/10.1007/s11032-013-9945-6

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