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Association mapping and meta-analysis: two complementary approaches for the detection of reliable Septoria tritici blotch quantitative resistance in bread wheat (Triticum aestivum L.)

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Abstract

Septoria tritici blotch (STB), caused by the ascomycete Mycosphaerella graminicola, is one of the most ubiquitous and important diseases of bread wheat worldwide. The aim of this study was to identify markers linked to loci conferring resistance to STB from seven biparental populations. Linkage analysis, meta-analysis and association mapping were combined to identify robust quantitative trait loci (QTLs) for resistance. Linkage analysis led to the detection of 115 QTLs for resistance to STB and 66 QTLs linked to plant height and/or earliness. Meta-analysis clustered these 115 QTLs into 27 Meta-QTLs (MQTLs) of pathogen resistance, of which 14 were found to be linked to plant height and/or earliness. Both the relationship between dwarfing and susceptibility to STB and the significant negative correlation between earliness and STB symptoms were confirmed. Eleven loci were linked to STB resistance by association mapping using a general linear model and/or a mixed linear model, of which eight co-located with STB MQTLs and two co-located with individual QTLs. Associated markers located in MQTL regions enhanced the relevance of the results and validated the potential of an association mapping approach. With several biparental populations, meta-analysis is the most relevant form of genetic analysis study, but association mapping can be used as a validation method. Regions linked to resistance in both methods should be relevant for use in breeding programs for improving resistance to STB in wheat varieties. The main interest in comparing both approaches is to detect robust loci that will be functional in many genetic backgrounds rather than just in one or a few specific backgrounds.

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Acknowledgments

This research was carried out with the financial support of the French Fonds de Soutien à l’Obtention Végétale (FSOV) within the program FSOV 2008B “Exploitation de résistances durables aux septorioses et fusarioses de blé tendre” and with the financial contribution of the European Community within the project BioExploit (integrated project FOOD-CT-2005-513959) “exploitation of natural plant biodiversity for pesticide-free production of food”. We thank Drs. Steve Barnes and Glenda Willems, SESVanderHave Company, for their critical review of the manuscript.

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  1. Seyed Mahmod Tabib Ghaffary

    Present address: Safiabad Agricultural Research Center, P.O. Box 333, Dezfoul, Iran

Authors and Affiliations

  1. Bioplante, 3 rue Florimond Desprez, BP 41, 59242, Cappelle-en-Pévèle, France

    Ellen Goudemand, Valérie Laurent, Laure Duchalais, Philippe Lonnet, Eric Margalé & Olivier Robert

  2. RAGT, 60 rue Léon Beauchamp, 59930, La Chapelle d’Armentières, France

    Laure Duchalais & Eric Margalé

  3. Plant Research International, Biointeractions and Plant Health, P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    Seyed Mahmod Tabib Ghaffary & Gert H. J. Kema

  4. Florimond Desprez, 3 rue Florimond Desprez, BP 41, 59242, Cappelle-en-Pévèle, France

    Ellen Goudemand, Philippe Lonnet & Olivier Robert

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Goudemand, E., Laurent, V., Duchalais, L. et al. Association mapping and meta-analysis: two complementary approaches for the detection of reliable Septoria tritici blotch quantitative resistance in bread wheat (Triticum aestivum L.). Mol Breeding 32, 563–584 (2013). https://doi.org/10.1007/s11032-013-9890-4

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