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Novel loci for field resistance to black-streaked dwarf and stripe viruses identified in a set of reciprocal introgression lines of rice (Oryza sativa L.)

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Abstract

Rice black-streaked dwarf virus (RBSDV) and stripe virus (RSV) are the two chronic viral diseases causing great damage to rice (Oryza sativa L.) production in China, and both are transmitted by the small brown planthopper (SBPH, Laodelphax striatellus Fallén). Quantitative trait loci (QTL) affecting field resistance to these two viral diseases were identified using QTL mapping software in a set of reciprocal introgression lines derived from the cross between Lemont and Teqing. A panel of 119 landraces was used for marker confirmation and allele mining. A total of 17 quantitative resistance loci (QRL) for the infection incidences of RBSDV and RSV were discovered and belong to 16 regions on all chromosomes except chromosome 12. Among them, 12 QRL were confirmed by association mapping, and many novel alleles at these loci were mined from the set of landraces. Only one region was found to be responsible for the genetic overlap between the field resistance against RBSDV and RSV, which was reported to be associated with SBPH resistance. The favorable alleles at the above novel and/or overlapping loci should be effective for marker-assisted selection breeding for resistance against the two diseases and the insect. Different strategies of varietal development and effective deployment against the two viral diseases are also discussed.

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Acknowledgments

We thank Prof. Bao-Rong Lu from Fudan University for kindly providing us with the landraces used in this study. We are also grateful to Mr. Da-You Zhang from the Institute of Yandu Agricultural Sciences, Jiangsu province, for his assistance in the field tests. This research was funded by the project no. 31000700, from the National Science Foundation of China; the “Green Super Rice” project no. 2010AA101803 (the Chinese Ministry of Science & Technology); the “948” program no. 2010-G2B (Ministry of Agriculture of PR China); National Science & Technology Key Specific Projects for Transgenic Variety Development no. 2009ZX08001-005B, 019 Ministry of Agriculture of PR China); the Jiangsu Independent Innovation Project no. CX(11)4020 and CX(11)4022, a project (BK2009321) from the Provincial Science Foundation, Jiangsu Province.

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Authors and Affiliations

  1. Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, 12 South Zhong-Guan-Cun St., Beijing, 100081, China

    Tianqing Zheng, Huqu Zhai, Linghua Zhu, Veronica N. E. Uzokwe, Jianlong Xu & Zhikang Li

  2. Institute of Food Crops, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Jie Yang, Weigong Zhong, Fangjun Fan, Jinhuan Yang, Jun Wang & Jinyan Zhu

  3. International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

    A. Jauhar Ali & Zhikang Li

Authors
  1. Tianqing Zheng
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  2. Jie Yang
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  3. Weigong Zhong
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  4. Huqu Zhai
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  5. Linghua Zhu
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  6. Fangjun Fan
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  7. A. Jauhar Ali
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  8. Jinhuan Yang
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  9. Jun Wang
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  10. Jinyan Zhu
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  11. Veronica N. E. Uzokwe
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  12. Jianlong Xu
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  13. Zhikang Li
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Corresponding authors

Correspondence to Jianlong Xu or Zhikang Li.

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Tianqing Zheng and Jie Yang are contributed equally to this work.

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Zheng, T., Yang, J., Zhong, W. et al. Novel loci for field resistance to black-streaked dwarf and stripe viruses identified in a set of reciprocal introgression lines of rice (Oryza sativa L.). Mol Breeding 29, 925–938 (2012). https://doi.org/10.1007/s11032-011-9691-6

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  • DOI: https://doi.org/10.1007/s11032-011-9691-6

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