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Discovery, localization, and sequence characterization of molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.)

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Abstract

Molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.) were identified using near-isogenic lines and bulked segregant analysis. Six markers for Pc48, the closest being 6 cM away, were found in a ‘Pendek-39’ × ‘Pendek-48’ (Pendek3948) population, but none was found in a ‘Pendek-48’ × ‘Pendek-38’ (Pendek4838) population. Three markers for Pc39 were found in the Pendek3948 population, one of which cosegregated with the gene. This same marker was found to be 6 cM away from the gene in an ‘OT328’ × ‘Dumont’ (OT328Du) population. Nine markers for Pc38 were found in the Pendek4838 population, eight of which are within 2 cM of the gene. One other marker for Pc38 was found in the OT328Du population; however, comparative mapping suggests that the Pc38 region in OT328Du is in a different location than that in Pendek4838. A number of markers unlinked to the genes under study formed linkage groups in both the Pendek3948 and Pendek4838 populations. Four of these show homology or homoeology to each other and to the Pc39 region in Pendek3948. Two RFLP clones closely linked to Pc38 code for a putative leucine-rich repeat transmembrane protein kinase and a cre3 resistance gene analogue. This study provides information to support molecular breeding in oat, and contributes to ongoing research into genomic regions associated with fungal pathogen resistance.

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Author notes

  1. Louise S. O’Donoughue

    Present address: DNA Landmarks, Inc., 84 rue Richelieu, QC J3B 6X3, St-Jean sur Richelieu, Canada

Authors and Affiliations

  1. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, 960 Carling Ave., ON K1A 0C6, Ottawa, Canada

    Charlene P. Wight, Louise S. O’Donoughue, Nicholas A. Tinker & Stephen J. Molnar

  2. Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Rd., MB R3T 2M9, Winnipeg, Canada

    James Chong

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  1. Charlene P. Wight
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Correspondence to Stephen J. Molnar.

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Wight, C.P., O’Donoughue, L.S., Chong, J. et al. Discovery, localization, and sequence characterization of molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.). Mol Breeding 14, 349–361 (2005). https://doi.org/10.1007/s11032-005-0148-7

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