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Chronic inflammatory pain upregulates expression of P2Y2 receptor in small-diameter sensory neurons

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Abstract

Roles of ionotropic purinergic (P2X) receptors in chronic pain have been intensively investigated. However, the contribution of metabotropic purinergic (P2Y) receptors to pathological pain is controversial. In the present study, using single cell RT-PCR (reverse transcription-polymerase chain reaction) and single cell nested-PCR techniques, we examined the expression of P2X2, P2X3, P2Y1 and P2Y2 mRNA transcripts in retrogradely labeled cutaneous sensory neurons from mouse lumber dorsal root ganglia (DRGs) following peripheral inflammation. The percentage of cutaneous sensory neurons expressing P2Y2 mRNA transcripts increased after complete Freund’s adjuvant (CFA) treatment. Particularly, the P2Y2 mRNA transcripts were more frequently detected in small-diameter cutaneous neurons from CFA-treated mice than those from control mice. Coexpression of P2Y2 and P2X (P2X2 or P2X3) mRNAs was more frequently observed in cutaneous sensory neurons from CFA-treated mice relative to controls. Pain behavioral tests showed that the blockade of P2Y receptors by suramin attenuated mechanical allodynia evoked either by CFA or uridine triphosphate (UTP), an endogenous P2Y2 and P2Y4 agonist. These results suggest that chronic inflammatory pain enhances expression of P2Y2 receptor in peripheral sensory neurons that innervate the injured tissue and the activation of P2Y receptors contributes to mechanical allodynia following inflammation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (81100822), Zhejiang Provincial Natural Science Foundation of China (LY15H090011), Leading and top Talents Project of Ningbo City (ZX2012000399) and Sponsored by K.C.Wong Magna Fund in Ningbo University.

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All authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China

    Huiqin Zhu, Yi Yu, Lingyan Zheng, Lu Wang, Chenli Li, Jiangyuan Yu, Jing Wei, Chuang Wang, Junfang Zhang, Shujun Xu, Xiaofei Wei, Wei Cui, Qinwen Wang & Xiaowei Chen

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  1. Huiqin Zhu
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  2. Yi Yu
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  3. Lingyan Zheng
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  4. Lu Wang
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  5. Chenli Li
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  6. Jiangyuan Yu
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  7. Jing Wei
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  9. Junfang Zhang
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  10. Shujun Xu
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  11. Xiaofei Wei
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  12. Wei Cui
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  13. Qinwen Wang
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  14. Xiaowei Chen
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Correspondence to Xiaowei Chen.

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Zhu, H., Yu, Y., Zheng, L. et al. Chronic inflammatory pain upregulates expression of P2Y2 receptor in small-diameter sensory neurons. Metab Brain Dis 30, 1349–1358 (2015). https://doi.org/10.1007/s11011-015-9695-8

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  • DOI: https://doi.org/10.1007/s11011-015-9695-8

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