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Ischemic colitis of the colon in streptozotocin-induced diabetic rats

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Abstract

This study focuses on two inflammatory diseases, viz., “diabetes mellitus (DM)” that causes serious complications such as retinopathy, nephropathy, and neuropathy, and “ischemic colitis” which is evoked by DM. Ischemic colitis originates from the reduction in mesenteric blood flow to the colon with existence of the occlusive or non-occlusive reasons. Our study objective was to provide early diagnostic approach for ischemic colitis in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley rats were divided into four groups: (i) control use of 0.1 M citrate buffer, the solvent of streptozotocin (C), (ii). induced ischemia (I), (iii) rats subjected to 60 mg/kg STZ intraperitoneally to induce type 1 diabetes (D) (48 h after STZ injection, blood glucose levels >200 mg/dl were considered as diabetic), and (iv) diabetic rats subjected to intestinal ischemia (D+I). The third diabetic group (D) was not operated. At the end of the experimental period, rats were sacrificed, C-reactive protein (CRP) and calprotectin levels were measured in the serum and colon tissue specimens. Tissue specimens were also analyzed histologically. We found that serum and colon calprotectin levels were elevated in the D+I group compared to the D and/or I group alone, but relatively calprotectin levels increased in I as compared to C group in colon tissues. CRP levels were significantly increased with ischemic colitis in diabetes, while colon CRP levels were decreased. These results provide evidence for the existence of inflammation in the STZ-induced diabetic rats with ischemic colitis. In conclusion, our measurements of serum calprotectin levels of STZ-induced diabetic rats with ischemic colitis provide a practical approach for an early diagnosis of ischemic colitis. Furthermore, these biochemical analyses correlate well with the histopathologic findings of STZ-induced diabetic rats with ischemic colitis. Future studies would be desirable to further strengthen the role of calprotectin in the early diagnosis of ischemic colitis in diabetics clinical settings.

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References

  1. Kooti W, Farokhipour M, Asadzadeh Z, Larky DA, Samani MA (2016) The role of medicinal plants in the treatment of diabetes: a systematic review. Electr Phys 8:1832–1842

    Article  Google Scholar 

  2. Rochette L, Zeller M, Cottin Y, Vergely C (2014) Diabetes, oxidative stress and therapeutic strategies. Biochim Biophys Acta 1840:2709–2729

    Article  CAS  PubMed  Google Scholar 

  3. Yi B, Huang G, Zhou Z (2016) Different role of zinc transporter 8 between type 1 diabetes mellitus and type 2 diabetes mellitus. J Diabetes Investig 7:459–465

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Pedersen L, Nybo M, Poulsen MK, Henriksen JE, Dahl J, Rasmussen LM (2014) Plasma calprotectin and its association with cardiovascular disease manifestations, obesity and the metabolic syndrome in type 2 diabetes mellitus patients. BMC Cardiovasc Disord 14:196. doi:10.1186/1471-2261-14-196

    Article  PubMed  PubMed Central  Google Scholar 

  5. Testa R, Bonfigli AR, Genovese S, Nigris VD, Ceriello A (2014) The possible role of flavonoids in the prevention of diabetic complications. Nutrients 8(5):310. doi:10.3390/nu8050310

    Article  Google Scholar 

  6. Qiu Y, Deng F, Li M, Lei S (2014) Identification of novel risk genes associated with type 1 diabetes mellitus using a genome-wide gene-based association analysis. J Diabetes Investig 5:649–656

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Yosaee S, Fakhrabadi MA, Shidfar F (2016) Positive evidence for vitamin A role in prevention of type 1 diabetes. World J Diabetes 7:177–188

    Article  PubMed  PubMed Central  Google Scholar 

  8. Petzold A, Solimena M, Knoch KP (2015) Mechanisms of beta cell dysfunction associated with viral infection. Curr Diab Rep 15:73–82

    Article  PubMed  PubMed Central  Google Scholar 

  9. Karayiannakis AJ, Bolanaki H, Kouklakis G, Dimakis K, Memet İ, Simopoulos C (2011) Ischemic colitis of the left colon in a diabetic patient. Case Rep Gastroenterol 5:239–245

    Article  PubMed  PubMed Central  Google Scholar 

  10. Nagai T, Tomizawa T, Monden T, Mori M (1998) Diabetes mellitus accompanied by non-occlusive colonic ischemia. Intern Med 37:454–456

    Article  CAS  PubMed  Google Scholar 

  11. Slone EA, Fleming SD (2014) Membrane lipid interactions in intestinal ischemia/reperfusion-induced injury. Clin Immunol 153:228–240

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Moschetta M, Telegrafo M, Rella L, Lamora AAS, Angelelli G (2014) Multi-detector CT features of acute intestinal ischemia and their prognostic correlations. World J Radiol 6:130–138

    Article  PubMed  PubMed Central  Google Scholar 

  13. Hefaiedh R, Sabbah M, Ennaifer R et al (2013) Ischemic colitis in five points: an update 2013. La Tunisie Med 92:299–303

    Google Scholar 

  14. Mohanapriya T, Singh KB, Arulappan T, Shobhana R (2012) Ischemic colitis. Indian J Surg 74:396–400

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Washington C, Carmichael JC (2012) Management of ischemic colitis. Clin Colon Rectal Surg 25:228–235

    Article  PubMed  PubMed Central  Google Scholar 

  16. Kayama Y, Raaz U, Jagger A et al (2015) Diabetic cardiovascular disease induced by oxidative stress. Int J Mol Sci 16:25234–25263

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Giffen PS, Turton J, Andrews CM et al (2003) Markers of experimental acute inflammation in the Wistar Han rat with particular reference to haptoglobin and C-reactive protein. Arch Toxicol 77:392–402

    Article  CAS  PubMed  Google Scholar 

  18. Cury DB, Mizsputen SJ, Versolato C et al (2013) Serum calprotectin levels correlate with biochemical and histological markers of disease activity in TNBS colitis. Cell Immunol 282:66–70

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Stoccaa A, O’Toole D, Hynes N et al (2012) A role for MRP8 in in stent restenosis in diabetes. Atherosclerosis 221:325–332

    Article  Google Scholar 

  20. Bouma G, Lam-Tse WK, Wierenga-Wolf AF, Drexhage HA, Versnel MA (2004) Increased serum levels of MRP-8/14 in type 1 diabetes induce an increased expression of CD11b and an enhanced adhesion of circulating monocytes to fibronectin. Diabetes 53:1979–1986

    Article  CAS  PubMed  Google Scholar 

  21. Steiner JM (2014) Review of commonly used clinical pathology parameters for general gastrointestinal disease with emphasis on small animals. Toxicol Pathol 42:189–194

    Article  PubMed  Google Scholar 

  22. Chang S, Malter L, Hudesman D (2015) Disease monitoring in inflammatory bowel disease. World J Gastroenterol 21:11246–11259

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Lin N, Shi JJ, Li YM et al (2016) What is the impact of n-3 PUFAs on inflammation markers in Type 2 diabetic mellitus populations?: a systematic review and meta-analysis of randomized controlled trials. Lipids Health Dis 15:133. doi:10.1186/s12944-016-0303-7

    Article  PubMed  PubMed Central  Google Scholar 

  24. Zheng N, Shi X, Chen X, Lv W (2015) Associations between inflammatory markers, hemostatic markers, and microvascular complications in 182 Chinese patients with type 2 diabetes mellitus. Lab Med 46:214–220

    Article  PubMed  Google Scholar 

  25. Syed Ikmal SI, Zaman Huri H, Vethakkan SR, Wan Ahmad WA (2013) Potential biomarkers of insulin resistance and atherosclerosis in type 2 diabetes mellitus patients with coronary artery disease. Int J Endocrinol 2013:698567. doi:10.1155/2013/698567

    Article  PubMed  PubMed Central  Google Scholar 

  26. Ghasemi-Niri SF, Maqbool F, Baeeri M, Gholami M, Abdollahi M (2016) Phosalone-induced inflammation and oxidative stress in the colon: evaluation and treatment. World J Gastroenterol 22:4999–5011

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Luján-Sanchis M, Sanchis-Artero L, Larrey-Ruiz L et al (2016) Current role of capsule endoscopy in Crohn’s disease. World J Gastrointest Endosc 8:572–583

    Article  PubMed  PubMed Central  Google Scholar 

  28. Zhang X, Wu J, Ye B, Wang Q, Xie X, Shen H (2016) Protective effect of curcumin on TNBS-induced intestinal inflammation is mediated through the JAK/STAT pathway. BMC Complement Altern Med 16:299. doi:10.1186/s12906-016-1273-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Theodoropoulou A, Κoutroubakis IE (2008) Ischemic colitis: clinical practice in diagnosis and treatment. World J Gastroenterol 14:7302–7308

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Istanbul General Surgery Clinic, Umraniye Research and Training Hospital, Istanbul, Turkey

    Yahya Ozel, Ilyas Kudas & Serife Aktas

  2. Department of Pharmacology, Faculty of Pharmacy, Marmara University, Tıbbiye St., No: 49, Haydarpaşa, 34688, Istanbul, Turkey

    H. Kubra Elcioglu, Z. Ayca Cevikelli & Levent Kabasakal

  3. Florida Hospital Medical Center, Orlando, FL, 32804, USA

    Sarfraz Ahmad

  4. Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey

    Hafize Uzun

  5. Department of Pathology, Istanbul General Surgery Clinic, Umraniye Research and Training Hospital, Istanbul, Turkey

    Cumhur Topal

Authors
  1. Yahya Ozel
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  2. H. Kubra Elcioglu
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  3. Z. Ayca Cevikelli
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  4. Ilyas Kudas
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  5. Sarfraz Ahmad
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  6. Hafize Uzun
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  7. Cumhur Topal
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  8. Serife Aktas
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  9. Levent Kabasakal
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Correspondence to H. Kubra Elcioglu or Sarfraz Ahmad.

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None of the authors has any potential financial or commercial conflict of interest associated with this research manuscript.

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Ozel, Y., Elcioglu, H.K., Cevikelli, Z.A. et al. Ischemic colitis of the colon in streptozotocin-induced diabetic rats. Mol Cell Biochem 439, 87–93 (2018). https://doi.org/10.1007/s11010-017-3138-2

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  • DOI: https://doi.org/10.1007/s11010-017-3138-2

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