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Proteomic analysis of mitochondrial proteins in the guinea pig heart following long-term normobaric hyperoxia

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Abstract

Normobaric hyperoxia is applied for the treatment of a wide variety of diseases and clinical conditions related to ischemia or hypoxia, but it can increase the risk of tissue damage and its efficiency is controversial. In the present study, we analyzed cardiac mitochondrial proteome derived from guinea pigs after 60 h exposure to 100% molecular oxygen (NBO) or O2 enriched with oxygen cation (NBO+). Two-dimensional gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry identified twenty-two different proteins (among them ten nonmitochondrial) that were overexpressed in NBO and/or NBO+ group. Identified proteins were mainly involved in cellular energy metabolism (tricarboxylic acid cycle, oxidative phosphorylation, glycolysis), cardioprotection against stress, control of mitochondrial function, muscle contraction, and oxygen transport. These findings support the viewpoint that hyperoxia is associated with cellular stress and suggest complex adaptive responses which probably contribute to maintain or improve intracellular ATP levels and contractile function of cardiomyocytes. In addition, the results suggest that hyperoxia-induced cellular stress may be partially attenuated by utilization of NBO+ treatment.

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Acknowledgements

This work was partially supported by Grant VEGA 1/0129/14, project “Biomedical Center Martin,” ITMS code: 26220220187 co-financed from EU sources and project “Center of Translational Medicine,” ITMS code 26220220021, co-financed from EU sources.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Comenius University in Bratislava, Jessenius Faculty of Medicine, Martin, Slovakia

    Lucia Lichardusova, Zuzana Tatarkova, Peter Racay, Jan Lehotsky & Peter Kaplan

  2. Department of Physiology, Comenius University in Bratislava, Jessenius Faculty of Medicine, Martin, Slovakia

    Andrea Calkovska & Daniela Mokra

  3. Biomedical Center Martin, Comenius University in Bratislava, Jessenius Faculty of Medicine, Mala Hora 4D, SK-036 01, Martin, Slovakia

    Andrea Calkovska, Daniela Mokra, Peter Racay, Jan Lehotsky & Peter Kaplan

  4. Department of Physiology, PJ Safarik University, Faculty of Medicine, Kosice, Slovakia

    Ivan Engler

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  1. Lucia Lichardusova
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  2. Zuzana Tatarkova
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Correspondence to Peter Kaplan.

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Lichardusova, L., Tatarkova, Z., Calkovska, A. et al. Proteomic analysis of mitochondrial proteins in the guinea pig heart following long-term normobaric hyperoxia. Mol Cell Biochem 434, 61–73 (2017). https://doi.org/10.1007/s11010-017-3037-6

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