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MicroRNA-126 attenuates palmitate-induced apoptosis by targeting TRAF7 in HUVECs

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Abstract

The aim of the present study was to explore the role of miR-126 in palmitate-induced HUVECs apoptosis and the possible mechanisms. Palmitate inhibited miR-126 expression in HUVECs, increased reactive oxygen species (ROS) production, and induced apoptosis as determined by up-regulation of caspase-3 activity and DNA fragmentation. Overexpression of miR-126 decreased ROS production, TNF-α expression, and apoptosis in palmitate-stimulated HUVECs. In contrast, miR-126 antagomir enhanced palmitate-induced ROS production, TNF-α expression, and apoptosis. The induction of miR-126 correlated with a reduction in TRAF7. We further showed that miR-126 targeted and inhibited TRAF7 expression through target sites located in the 3′ untranslated region of TRAF7 mRNA. In concordance, miR-126 mimic reduced TRAF7 protein in HUVECs, whereas the inhibition of miR-126 increased it. This study demonstrates an anti-apoptotic role of miR-126 in HUVECs and identifies TRAF7 as a direct target of miR-126 in HUVECs.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81070232, 81270372, 81470568), Key Project of Chinese Ministry of Education (No. 212077), Grants for Scientific Research of BSKY (No. XJ201107, XJ2008015) from Anhui Medical University.

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Authors and Affiliations

  1. Institute of Clinical Pharmacology, Anhui Medical University, Hefei, 230032, Anhui, People’s Republic of China

    Yi Wang, Wei Wei, Yuan Wang & Huaqing Zhu

  2. Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei, 230032, Anhui, People’s Republic of China

    Yi Wang, Feng Wang, Yan Wu, Li Zuo, Sumei Zhang, Qing Zhou, Yuan Wang & Huaqing Zhu

  3. Key Laboratory of Gene Research of Anhui Province, Hefei, Anhui, People’s Republic of China

    Yi Wang, Yan Wu, Li Zuo, Sumei Zhang, Yuan Wang & Huaqing Zhu

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  1. Yi Wang
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  2. Feng Wang
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  3. Yan Wu
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  4. Li Zuo
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  6. Qing Zhou
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  7. Wei Wei
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  8. Yuan Wang
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  9. Huaqing Zhu
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Correspondence to Yuan Wang or Huaqing Zhu.

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Wang, Y., Wang, F., Wu, Y. et al. MicroRNA-126 attenuates palmitate-induced apoptosis by targeting TRAF7 in HUVECs. Mol Cell Biochem 399, 123–130 (2015). https://doi.org/10.1007/s11010-014-2239-4

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  • DOI: https://doi.org/10.1007/s11010-014-2239-4

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