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Cloning and expression pattern of akirin2 gene in broiler

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Abstract

Akirin2 is an important nuclear factor which plays functions in innate immune response, myogenesis, muscle development, and carcinogenesis. In this study, akirin2 genes were cloned from 4-day-old Sanhuang and AA+ broiler, and its expression patterns were analyzed by RT-PCR. The results showed that there were four SNPs in the 5′-terminal region of akirin2 coding sequences. Expression profile analysis showed that the akirin2 transcripts were constitutively expressed in 15 tissues tested, and similar expression patterns were found between the two breeds of broilers. In addition, one of the interesting findings was that the akirin2 gene is highly expressed in blood and lowly expressed in heart, respectively. These data can serve as a foundation for further studying functions of akirin2 gene.

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Fig. 1
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Abbreviations

AA:

Amino acid

CDS:

Coding sequence

SNP:

Single nucleotide polymorphism

NF-κB:

Nuclear factor-kappa B

TLR:

Toll-like receptor

TNF:

Tumor necrosis factor

IL-1β:

Interleukin 1-β

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Acknowledgments

This study was supported by foundation for Natural Science Foundation of the Heilongjiang Province (No. C201135), Harbin Technological Innovation Special Fund Research Project (No. RC2012QN002008), and Pre-research project of Harbin Normal University (No. 12XYG-08).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. College of Life Science and Technology, Harbin Normal University, Harbin, 150001, People’s Republic of China

    Yang Chang, Weitao Mu & Dongxue Zhao

  2. College of Life Science and Technology, Harbin Normal University, Harbin, 150025, Heilongjiang Province, People’s Republic of China

    Chaolai Man

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  1. Chaolai Man
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  2. Yang Chang
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  4. Dongxue Zhao
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Correspondence to Chaolai Man.

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Man, C., Chang, Y., Mu, W. et al. Cloning and expression pattern of akirin2 gene in broiler. Mol Cell Biochem 397, 61–65 (2014). https://doi.org/10.1007/s11010-014-2172-6

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  • DOI: https://doi.org/10.1007/s11010-014-2172-6

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