Abstract
Vestibular schwannomas (VSs) are benign tumors arising from eighth cranial nerve and most often occur sporadically in individuals of middle age group. Sporadic VSs are rarely reported in the young population. In this study, we evaluated clinical behaviors of 12 young sporadic VSs by the statistical comparison with a matched series of 145 adult cases. We found that young tumors were characterized by an earlier onset of initial symptom, shorter duration from the first symptom to diagnosis, and larger tumor size than adult ones. Standard sequencing demonstrated the presence of NF2 mutations in eight tumors. All NF2 mutations identified were truncating mutations (nonsense, frameshift, and splicing-site mutations). Earlier formation of VSs in young patients was evidenced by the high incidence of NF2 mutations (66.7 %) far beyond our previous study in the adult case series (34.5 %). Furthermore, young tumors exhibited deficient merlin or heightened phosphorylated merlin that was subsequently demonstrated to be well correlated with increased tumor size. Finally, we compared protein levels of four pathogenesis-related molecules between young and adult group but there was no significant difference. These results led us to suggest that high frequency of NF2 mutations may play a critical role in early tumorigenesis of young VSs. Moreover, merlin deficiency or phosphorylation status of merlin was involved in their earlier development. Further study remains to fully understand the mechanism for the rapid growth of young VSs.
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Gutmann DH, Aylsworth A, Carey JC et al (1997) The diagnostic evaluation and multidisciplinary management of neurofibromatosis and neurofibromatosis 2. JAMA 278:51–57
Jacoby LB, MacCollin M, Louis DN et al (1994) Exon scanning for mutation of the NF2 gene in schwannomas. Hum Mol Genet 3:413–419
Cayé-Thomasen P, Borup R, Stangerup SE et al (2010) Deregulated genes in sporadic vestibular schwannomas. Otol Neurotol 31:256–266
Ahmad Z, Brown CM, Patel AK et al (2010) Merlin knockdown in human Schwann cells: clues to vestibular schwannoma tumor genesis. Otol Neurotol 31:460–466
Hadfield KD, Smith MJ, Urquhart JE et al (2010) Rates of loss of heterozygosity and mitotic recombination in NF2 schwannomas, sporadic vestibular schwannomas and schwannomatosis schwannomas. Oncogene 29:6216–6221
Zhang Zhihua, Wang Zhaoyan, Sun Lianhua et al (2013) Mutation spectrum and differential gene expression in cystic and solid vestibular schwannoma. Genet Med. doi:10.1038/gim.2013.114
Bretscher A, Edwards K, Fehon RG et al (2002) Proteins and merlin: integrators at the cell cortex. Nat Rev Mol Cell Biol 3:586–599
Li W, You L, Cooper J, Schiavon G et al (2010) Merlin/NF2 suppresses tumorigenesis by inhibiting the E3 ubiquitin ligase CRL4 DCAF1 in the nucleus. Cell 140:477–490
James MF, Han S, Polizzano C et al (2010) NF2/merlin is a novel negative regulator of mTOR complex 1, and activation of mTORC1 is associated with meningioma and schwannoma growth. Mol Cell Biol 29:4250–4261
Lallemand D, Manent J, Couvelard A et al (2009) Merlin regulates transmembrane receptor accumulation and signaling at the plasma membrane in primary mouse Schwann cells and in human schwannomas. Oncogene 28:854–865
Hamaratoglu F, Willecke M, Kango-Singh M et al (2006) The tumour-suppressor genes NF2/Merlin and Expanded act through Hippo signalling to regulate cell proliferation and apoptosis. Nat Cell Biol 8:27–36
Okada T, Lopez-Lago M, Giancotti FG (2006) Merlin/NF-2 mediates contact inhibition of growth by suppressing recruitment of Rac to the plasma membrane. J Cell Biol 171:361–371
Rong R, Surace EI, Haipek CA et al (2004) Serine 518 phosphorylation modulates merlin intramolecular as sociation and binding to critical effectors important for NF2 growth suppression. Oncogene 23:8447–8454
Jin H, Sperka T, Herrlich P et al (2006) Tumorigenic transformation by CPI-17 through inhibition of a merlin phosphatase. Nature 442:576–579
Yang C, Asthagiri AR, Iyer RR et al (2011) Missense mutations in the NF2 gene result in the quantitative loss of merlin protein and minimally affect protein intrinsic function. Proc Natl Acad Sci USA 108:4980–4985
Walcott BP, Sivarajan G, Bashinskaya B et al (2009) Sporadic unilateral vestibular schwannoma in the pediatric population. J Neurosurg Pediatr 4:125–129
House JW, Brackmann DE (1985) Facial nerve grading system. Otolaryngol Head Neck Surg 93:146–147
Wu H, Chen Y, Wang ZY et al (2010) Involvement of p21 (waf1) in merlin deficient sporadic vestibular schwannomas. Neuroscience 170:149–155
Wang Zhaoyan, Yanjun Lu, Tang Juanjuan et al (2009) The phosphorylation status of merlin in sporadic vestibular Schwannomas. Mol Cell Biochem 324:201–206
Dayalan AH, Jothi M, Keshava R et al (2006) Age dependent phosphorylation and deregulation of p53 in human vestibular schwannomas. Mol Carcinog 45:38–46
Xiao GH, Gallagher R, Shetler J et al (2005) The NF2 tumor suppressor gene product, merlin, inhibits cell proliferation and cell cycle progression by repressing cyclin D1 expression. Mol Cell Biol 25(6):2384–2394
Kim H, Kwak NJ, Lee JY et al (2004) Merlin neutralizes the inhibitory effect of Mdm2 on p53. J Biol Chem 279:7812–7818
Lasak JM, Welling DB, Akhmametyeva et al (2002) Retinoblastoma-cyclin-dependent kinase pathway deregulation in vestibular schwannomas. Laryngoscope 112:1555–1561
Propp JM, McCarthy BJ, Davis FG et al (2006) Descriptive epidemiology of vestibular schwannomas. Neuro Oncol 8:1–11
Neary W, Newton VE, Laiode-Kemp SN et al (1996) A clinical, genetic and audiological study of patients with unilateral vestibular schwannomas. I. Clinical features of neurofibromatosis in patients with unilateral vestibular schwannomas. J Laryngol Otol 110:634–640
Evans DGR, Lye R, Neary W et al (1999) Probability of bilateral disease in people presenting with a unilateral vestibular schwannoma. J Neurol Neurosurg Psychiatry 66:764–767
Matthies C, Samii M (1997) Management of 1000 vestibular schwannomas (Acoustic Neuromas): clinical presentation. Neurosurgery 40:1–10
Mirzayan MJ, Gerganov VM, Lüdemann W et al (2007) management of vestibular schwannomas in young patients-comparison of clinical features and outcome with adult patients. Childs Nerv Syst 23:891–895
Torres-Martin M, Lassaletta L, San-Roman-Montero J et al (2013) Microarray analysis of gene expression in vestibular schwannomas reveals SPP1/MET signaling pathway and androgen receptor deregulation. Int J Oncol 42:848–862
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This work was supported by the National Natural Science Foundation of China (Grant No. 81371086 to Zhaoyan Wang).
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Hongsai Chen and Xiaoman Zhang have contributed equally to this work.
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Chen, H., Zhang, X., Zhang, Z. et al. The role of NF2 gene mutations and pathogenesis-related proteins in sporadic vestibular schwannomas in young individuals. Mol Cell Biochem 392, 145–152 (2014). https://doi.org/10.1007/s11010-014-2011-9
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DOI: https://doi.org/10.1007/s11010-014-2011-9