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Proteomic analysis of rosiglitazone and guggulsterone treated 3T3-L1 preadipocytes

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Abstract

Adipogenesis is the differentiation of preadipocytes to adipocytes which is marked by the accumulation of lipid droplets. Adipogenic differentiation of 3T3-L1 cells is achieved by exposing the cells to Insulin, Dexamethasone and IBMX for 5–7 days. Thiazolidinedione drugs, like rosiglitazone are potent insulin sensitizing agents and have been shown to enhance lipid droplet formation in 3T3-L1 cells, a model cell line for preadipocyte differentiation. Guggulsterone is a natural drug extracted from the gum resin of tree Commiphora mukul. Guggulsterone has been shown to inhibit adipogenesis and induce apoptosis in 3T3-L1 cells. In this study we treated the 3T3-L1 preadipocytes with rosiglitazone and guggulsterone and assessed the protein expression profile using 2D gel electrophoresis-based proteomics to find out differential target proteins of these drugs. The proteins that were identified upon rosiglitazone treatment generally regulate cell proliferation and/or exhibit anti-inflammatory effect which strengthens its differentiation-inducing property. Guggulsterone treatment resulted in the identification of the apoptosis-inducing proteins to be up regulated which rightly is in agreement with the apoptosis-inducing property of guggulsterone in 3T3-L1 cells. Some of the proteins identified in our proteomic screen such as Galectin1, AnnexinA2 & TCTP were further confirmed by Real Time qPCR. Thus, the present study provides a better outlook of proteins being differentially regulated/expressed upon treatment with rosiglitazone and guggulsterone. The detailed study of the differentially expressed proteins identified in this proteomic screen may further provide the better molecular insight into the mode of action of these anti-diabetic drugs rosiglitazone and guggulsterone.

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Acknowledgments

We are sincerely thankful to Council of scientific and Industrial Research-Central Drug Research Institute (CSIR-CDRI) for its generous support in the completion of this project. This work was supported by CSIR grants HCP0004, OLP0008 and Department of Biotechnology (DBT) grant GAP00068 to AKT. Institutional communication number for this manuscript is 8377.

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  1. Drug Target Discovery and Development Division, CSIR-Central Drug Research Institute, New Campus Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, 226021, UP, India

    Pooja Pal, Jitendra K. Kanaujiya, Savita Lochab, Shashi B. Tripathi, Sabyasachi Sanyal & Arun K. Trivedi

  2. Division of Hematology and Oncology, University Hospital of Leipzig, Johannissallee 32A, 04103, Leipzig, Germany

    Gerhard Behre

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  1. Pooja Pal
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Correspondence to Arun K. Trivedi.

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P. Pal and J. K. Kanaujiya contributed equally to this work

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Pal, P., Kanaujiya, J.K., Lochab, S. et al. Proteomic analysis of rosiglitazone and guggulsterone treated 3T3-L1 preadipocytes. Mol Cell Biochem 376, 81–93 (2013). https://doi.org/10.1007/s11010-012-1551-0

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