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Expression profile of DNA damage signaling genes in 2 Gy proton exposed mouse brain

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Abstract

Exposure of living systems to radiation results in a wide assortment of lesions, the most significant of is damage to genomic DNA which alter specific cell functions including cell proliferation. The radiation induced DNA damage investigation is one of the important area in biology, but still the information available regarding the effects of proton is very limited. In this report, we investigated the differential gene expression pattern of DNA damage signaling genes such as damaged DNA binding, repair, cell cycle arrest, checkpoints and apoptosis using quantitative real-time RT-PCR in proton exposed mouse brain tissues. The expression profiles showed significant changes in DNA damage related genes in 2 Gy proton exposed mouse brain tissues as compared to control brain tissues. Furthermore, we also show that significantly increased levels of apoptotic related genes, caspase-3 and 8 activities in these cells, suggesting that in addition to differential expression of DNA damage genes, the alteration of apoptosis related genes may also contribute to the radiation induced DNA damage followed by programmed cell death. In summary, our findings suggest that proton exposed cells undergo severe DNA damage which in turn destabilize the chromatin stability.

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Acknowledgements

This work was supported by NASA funding NNX08BA47A: NCC-1-02038: NIH-1P20MD001822-1.

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Authors and Affiliations

  1. Department of Biology, Molecular Toxicology Laboratory, Center for Biotechnology & Biomedical Sciences, Norfolk State University, Norfolk, VA, 23504, USA

    Sudhakar Baluchamy, Prabakaran Ravichandran, Vani Ramesh, Joseph C. Hall & Govindarajan T. Ramesh

  2. Department of Biology, Texas Southern University, Houston, TX, 77004, USA

    Ayodotun Sodipe & Olufisayo Jejelowo

  3. Radiation Biophysics Laboratory and Human Adaptation & Countermeasures Division, NASA Johnson Space Center, Houston, TX, 77058, USA

    Ye Zhang & Honglu Wu

  4. Department of Radiation Medicine, Loma Linda University Medical Center, Loma Linda, CA, 92354, USA

    Daila S. Gridley

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  1. Sudhakar Baluchamy
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  2. Ye Zhang
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  10. Govindarajan T. Ramesh
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Correspondence to Govindarajan T. Ramesh.

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Baluchamy, S., Zhang, Y., Ravichandran, P. et al. Expression profile of DNA damage signaling genes in 2 Gy proton exposed mouse brain. Mol Cell Biochem 341, 207–215 (2010). https://doi.org/10.1007/s11010-010-0451-4

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  • DOI: https://doi.org/10.1007/s11010-010-0451-4

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