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Effects of diallyl disulfide (DADS) on expression of apoptosis associated proteins in androgen independent human prostate cancer cells (PC-3)

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Abstract

Prostate cancer is a leading cause of death among the aging men. Surgical or radiotherapy is effective when the cancer is confined to the prostate gland but once the cancer spreads beyond the pelvis even chemotherapy and hormonal ablation therapy fails in curing this disease. Our previous studies have shown that diallyl disulfide (DADS) induces cell cycle arrest and also induces apoptosis in PC-3 cells. And now the present study is focused to see whether there is an activation of caspase cascade pathway. Hence, in the present study the apoptotic effect of DADS is studied by Western blot analysis of caspase-3, -9, -10 and Bcl-2, Bad, and Bax protein. The Apoptotic cells were assessed by Hoechst 33342 staining with 25 and 40 μM concentrations of DADS for 24 h. The results have shown that DADS at 25 and 40 μM concentrations has induced the activation of caspases. There is a significant increase in the expression of caspases (3, 9, and 10). The proapoptotic protein Bax has significantly increased at 40 μM of DADS treatment and there is significant increase of Bad protein at both the concentration. Bcl-2 protein has significantly decreased in DADS treated cells. Therefore, the present investigation serves as evidence that DADS may be a therapeutic drug in the treatment of prostate cancer.

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Acknowledgments

The financial assistance provided by University of Madras as UWPFE fellowship to one of the authors Mr. K. Senthilkumar is gratefully acknowledged. This work was supported by University of Madras under University with potential for excellence HS10 project.

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Authors and Affiliations

  1. Department of Endocrinology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, Tamil Nadu, 600 113, India

    Ramachandran Gayathri, Dharmalingam Nandagopal Gunadharini, Kalimuthu Senthilkumar, Gunasekar Krishnamoorthy, Sivanantham Banudevi, Ramamoorthy Chandrakanth Vignesh & Jagadeesan Arunakaran

  2. Department of Biochemistry and Medical Genetics, Manitoba Institute of Cell Biology, Winnipeg, Canada, MBR3Eovq

    Arumugam Arunkumar

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  1. Ramachandran Gayathri
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  2. Dharmalingam Nandagopal Gunadharini
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  3. Arumugam Arunkumar
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  4. Kalimuthu Senthilkumar
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  5. Gunasekar Krishnamoorthy
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  6. Sivanantham Banudevi
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  8. Jagadeesan Arunakaran
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Correspondence to Jagadeesan Arunakaran.

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Gayathri, R., Gunadharini, D.N., Arunkumar, A. et al. Effects of diallyl disulfide (DADS) on expression of apoptosis associated proteins in androgen independent human prostate cancer cells (PC-3). Mol Cell Biochem 320, 197–203 (2009). https://doi.org/10.1007/s11010-008-9903-5

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  • DOI: https://doi.org/10.1007/s11010-008-9903-5

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