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Increased expression of hLRH-1 in human gastric cancer and its implication in tumorigenesis

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Abstract

Altered signaling pathways or deregulated transcription factors represent an important category of molecular events leading to aberrant gene regulation in gastric cancer, among which the role of WNT/β-catenin pathway remains unclear. LRH-1 is a critical transcription factor in controlling cell proliferation via crosstalk with the β-catenin signaling pathway. In order to gain a knowledge of the expression of hLRH-1v1 and hLRH-1 in gastric cancer, a Q-PCR analysis was carried out. Our results showed that in about 50 and 47.6% of 42 tested patients with gastric cancer, the mRNA expression of hLRH-1v1 and hLRH-1 was significantly upregulated, as compared with self-paired normal control, respectively. Besides, overexpression of hLRH-1 was shown to promote the proliferation of gastric adenocarcinoma cell SGC-7901 via induction of cyclin E1. Taken together, our present study demonstrated for the first time the increased expression of hLRH-1v1 and hLRH-1 in human gastric cancer, an alteration which may implicate in tumorigenesis.

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Abbreviations

LRH-1:

Liver receptor homolog 1

RT:

Reverse transcription

Q-PCR:

Real-time quantitative polymerase chain reaction

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Acknowledgments

We are grateful to Professor Jiliang Fu (Department of Medicine and Life Sciences, Tongji University, Shanghai 200092, China) and Zhimin He (Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China) for their constructive comments of the manuscript.

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Authors and Affiliations

  1. Research Center for Molecular Medicine, Institute of Laboratory Medicine, Fuzhou Dongfang Hospital, Fuzhou, Fujian, 350025, P.R. China

    Shui-Liang Wang, De-Zhu Zheng, Feng-Hua Lan, Xiao-Jun Deng, Jian Zeng & Zhong-Yong Zhu

  2. Department of General Surgery, Fuzhou Dongfang Hospital, Fuzhou, Fujian, 350025, P.R. China

    Cheng-Jin Li

  3. Department of Gastroenterology, Fuzhou Dongfang Hospital, Fuzhou, Fujian, 350025, P.R. China

    Rong Wang

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  1. Shui-Liang Wang
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  2. De-Zhu Zheng
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  3. Feng-Hua Lan
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  4. Xiao-Jun Deng
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  5. Jian Zeng
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  6. Cheng-Jin Li
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  7. Rong Wang
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  8. Zhong-Yong Zhu
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Corresponding author

Correspondence to Shui-Liang Wang.

Additional information

Grants support: Grants 2004J067 from the Young Scientific and Technical Innovation Foundation of Fujian Province, C0710038 from the Natural Science Foundation of Fujian Province of China, and 200638 from the Foundation of Fuzhou Dongfang Hospital.

The authors Shui-Liang Wang and De-Zhu Zheng contributed equally to this work.

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Wang, SL., Zheng, DZ., Lan, FH. et al. Increased expression of hLRH-1 in human gastric cancer and its implication in tumorigenesis. Mol Cell Biochem 308, 93–100 (2008). https://doi.org/10.1007/s11010-007-9616-1

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  • DOI: https://doi.org/10.1007/s11010-007-9616-1

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