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Proteomic identification of differently expressed proteins responsible for osteoblast differentiation from human mesenchymal stem cells

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Abstract

Human mesenchymal stem cells (hMSC) are a population of multipotent cells that can differentiate into osteoblasts, chondrocytes, adipocytes, and other cells. The exact mechanism governing the differentiation of hMSC into osteoblasts remains largely unknown. Here, we analyzed protein expression profiles of undifferentiated as well as osteogenic induced hMSC using 2-D gel electrophoresis (2-DE), mass spectrometry (MS), and peptide mass fingerprinting (PMF) to investigate the early gene expression in osteoblast differentiation. We have generated proteome maps of undifferentiated hMSC and osteogenic induced hMSC on day 3 and day 7. 2-DE revealed 102 spots with at least 2.0-fold changes in expression and 52 differently expressed proteins were successfully identified by MALDI-TOF-MS. These proteins were classified into 7 functional categories: metabolism, signal transduction, transcription, calcium-binding protein, protein degradation, protein folding and others. The expression of some identified proteins was confirmed by further RT-PCR analyses. This study clarifies the global proteome during osteoblast differentiation. Our results will play an important role in better elucidating the underlying molecular mechanism in hMSC differentiation into osteoblasts.

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Abbreviations

HMSC:

Human mesenchymal stem cells

MW:

Molecular weight

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30100188, 30571891), National Basic Research Program of China (2001CB509904), the Key Scientific and Technological Projects of Guangdong Province (2003A3020103, 2005A30201001), and the Key Scientific and Technological Projects of Guangzhou City, No. 2002U13E0011. We thank all colleagues of Center for Stem Cell Biology and Tissue Engineering of Sun Yat-sen University in China for the technical support.

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Correspondence to Andy Peng Xiang.

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11010_2007_9497_MOESM1_ESM.pdf

Fig. S1 The expressions of 7 protein spots on the 2-DE afterosteoblast differentiation. 0 d: undifferentiated hMSC. 3 d, 7 d: days after treating inducing media (PDF 84 kb)

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Zhang, AX., Yu, WH., Ma, BF. et al. Proteomic identification of differently expressed proteins responsible for osteoblast differentiation from human mesenchymal stem cells. Mol Cell Biochem 304, 167–179 (2007). https://doi.org/10.1007/s11010-007-9497-3

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