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The Effect of Vitamin a Pretreatment on Radiation Induced Alteration in Neutrophil Functions

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Abstract

The aim of this investigation was to determine whether pre-administration of vitamin A will be effective in preventing the radiation-induced decline in MPO-H2O2 system and the end product of reactive nitrogen species (NOx) in guinea pig. Animals were subjected to 612 cG of radiation and polimorfonuclear leukocytes were isolated and then NOx and myeloperoxidase activity were measured. In irradiated animals, a marked decrease in NOx level and myeloperoxidase activity have been found compared to control (p = 0.001 and p < 0.000 respectively). The application of vitamin A significantly improved the radiation-induced decrease (for both p < 0.00). In conclusion pre-treatment of vitamin A is efficient to protect against radiation induced alteration in polimorfonuclear leukocyte.

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References

  1. Podrez EA, Abu-Soud HM, Hazen SL: Myeloperoxidase-generated oxidants and atherosclerosis. Free Radic Biol Med 28: 1717–1725, 2000

    Article  PubMed  CAS  Google Scholar 

  2. Van Dalen CJ, Winterbourn CC, Senthilmohan R, Kettle AJ: Nitrite as a substrate and inhibitor of myeloperoxidase. J Biol Chem 275: 11638–11644, 2000

    Article  PubMed  CAS  Google Scholar 

  3. Gaut JP, Byun J, Tran HD, Lauber WM, Carroll JA: Hotchkiss RS, Belaaouaj A, Heinecke JW. Myeloperoxidase produces nitrating oxidants in vivo. J Clin Invest 109: 1311–1319, 2002

    PubMed  CAS  Google Scholar 

  4. Evans TJ, Buttery LD, Carpenter A, Springall DR, Polak JM, Cohen J: Cytokine-treated human neutrophils contain inducible nitric oxide synthase that produces nitration of ingested bacteria. Proc Natl Acad Sci USA 93: 9553–9558, 1996

    Article  PubMed  CAS  Google Scholar 

  5. Zhang R, Brennan ML, Shen Z, MacPherson JC, Schmitt D, Molenda CE, Hazen SL: Myeloperoxidase functions as a major enzymatic catalyst for initiation of lipid peroxidation at sites of inflammation. J Biol Chem 277: 46116–46122, 2002

    Article  PubMed  CAS  Google Scholar 

  6. Lorimore SA, Coates PJ, Scobie GE, Milne G, Wright EG: Inflammatory-type responses after exposure to ionizing radiation in vivo: a mechanism for radiation-induced bystander effects? Oncogene 20: 7085–7095, 2001

    Article  PubMed  CAS  Google Scholar 

  7. Shechmeister IL, Fishman M: The effect of ionizing radiation on phagocytosis and the bactericidal power of the blood. I. The effect of radiation on migration of leucocytes. J Exp Med 101: 259–274, 1955

    Article  PubMed  CAS  Google Scholar 

  8. Levenson SM, Gruber CA, Rettura G, Gruber DK, Demetriou AA, Seifter E: Supplemental vitamin A prevents the acute radiation-induced defect in wound healing. Ann Surg 200: 494–512, 1984

    Article  PubMed  CAS  Google Scholar 

  9. Redlich CA, Rockwell S, Chung JS, Sikora AG, Kelley M, Mayne ST: Vitamin A inhibits radiation-induced pneumonitis in rats. J Nutr 128: 1661–1664, 1998

    PubMed  CAS  Google Scholar 

  10. Voevodskaya NV, Vanin AF: Gamma-irradiation potentiates L-arginine-dependent nitric oxide formation in mice. Biochem Biophys Res Commun 186: 1423–1428, 1992

    Article  PubMed  CAS  Google Scholar 

  11. Cutts JH: Cell separation methods in haematology. Academic Press, New York: 3–9, 1970

    Google Scholar 

  12. Glowick SP, Kaplan SD: Methods in Enzymology. Academic Press, New York 769–770 pp, 1955

    Google Scholar 

  13. Bories PN, Bories C: Nitrate determination in biological fluids by an enzymatic one-step assay with nitrate reductase. Clin Chem 41: 904–907, 1995

    PubMed  CAS  Google Scholar 

  14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951

    PubMed  CAS  Google Scholar 

  15. Schaue D, Marples B, Trott KR: The effects of low-dose X-irradiation on the oxidative burst in stimulated macrophages. Int J Radiat Biol 78: 567–576, 2002

    Article  PubMed  CAS  Google Scholar 

  16. Konkabaeva AE, Bazeliuk LT: Effects of ionizing radiation on catecholamine level in experimental animals. Gig Sanit 6: 22–23, 2001

    PubMed  Google Scholar 

  17. Twining SS, Schulte DP, Wilson PM, Fish BL, Moulder JE: Vitamin A deficiency alters rat neutrophil function. J Nutr 127: 558–565, 1997

    PubMed  CAS  Google Scholar 

  18. Beyzadeoglu M, Balkan M, Demiriz M, Tibet H, Dirican B, Oner K, Pak Y: Protective effect of vitamin A on acute radiation injury in the small intestine. Radiat Med 15: 1–5, 1997

    PubMed  CAS  Google Scholar 

  19. Zhao W, Han Y, Zhao B, Hirota S, Hou J, Xin W: Effect of carotenoids on the respiratory burst of rat peritoneal macrophages. Biochim Biophys Acta 1381: 77–88, 1998

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science and Art, Gazi University, Ankara, Turkey

    Barbaros Balabanlı

  2. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, 06310, Turkey

    Nurten Türközkan & Husamettin Erdamar

  3. Tubitak, Ankara, Turkey

    Sema Balabanlı

  4. Department of Radiation Oncology, Faculty of Medicine, Gazi University, Ankara, Turkey

    Müge Akmansu

Authors
  1. Barbaros Balabanlı
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  2. Nurten Türközkan
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  3. Sema Balabanlı
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  4. Husamettin Erdamar
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  5. Müge Akmansu
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Correspondence to Nurten Türközkan.

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Balabanlı, B., Türközkan, N., Balabanlı, S. et al. The Effect of Vitamin a Pretreatment on Radiation Induced Alteration in Neutrophil Functions. Mol Cell Biochem 286, 103–105 (2006). https://doi.org/10.1007/s11010-005-9099-x

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  • DOI: https://doi.org/10.1007/s11010-005-9099-x

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