Abstract
Treatment of microsomes (preferentially enriched with endoplasmic reticulum) isolated from bovine pulmonary artery smooth muscle tissue with H2O2 (1 mM) markedly stimulated matrix metalloproteinase activity and also inhibited Na+ dependent Ca2+ uptake. Electron micrograph revealed that H2O2 (1 mM) does not cause any damage to the microsomes. MMP-2 and TIMP-2 were determined to be the ambient protease and corresponding antiprotease of the microsomes. Pretreatment with vitamin E (1 mM) and TIMP-2 (50 μg/ml) reversed the effect produced by H2O2 (1 mM) on Na+ dependent Ca2+ uptake in the microsomes. However, H2O2 (1 mM) caused changes in MMP-2 activity and Na+ dependent Ca2+ uptake were not reversed upon pretreatment of the microsomes with a low concentration of 5 μg/ml of TIMP-2 which otherwise reversed MMP-2 (1 μg/ml) mediated increase in 14C-gelatin degradation and inhibition of Na+ dependent Ca2+ uptake. Combined treatment of the microsomes with a low dose of MMP-2 (0.5 μg/ml) and H2O2 (0.5 mM) inhibited Na+ dependent Ca2+ uptake in the microsomes compared to the respective low dose of either of them. Direct treatment of TIMP-2 (5 μg/ml) with H2O2 (1 mM) abolished the inhibitory effect of the inhibitor on 14C-gelatinolytic activity elicited by 1 μg/ml of MMP-2. Thus, one of the mechanisms by which H2O2 activates MMP-2 could be due to inactivation of TIMP-2 by the oxidant. The resulting activation of MMP-2 subsequently inhibits Na+ dependent Ca2+ uptake in the microsomes. (Mol Cell Biochem 270: 79–87, 2005)
Similar content being viewed by others
References
Chakraborti T, Das S, Mandal M, Roychoudhury S, Chakraborti S: Oxidant, mitochondria and calcium: An overview. Cell Signal 10: 675–683, 1999
Chakraborti T, Ghosh SK, Michael JR, Batabyal SK, Chakraborti S: Targets of oxidative stress in the cardiovascular system. Mol Cell Biochem 187: 1–10, 1998
Chakraborti T, Das S, Mandal M, Mandal A, Chakraborti S: Ca2+ dynamics under oxidant stress in the cardiovascular system. In: K.B. Storey, J.M. Storey (eds). Cellular and Molecular Responses to Stress, Vol 2, Elsevier Science, New York, 2002, pp 213–228
Bose R, Li Y, Roberts D: Na+/Ca2+ exchange in activated and non activated human platelets. Ann NY Acad Sci 976: 350–353, 2002
Golovina VA, Blaustein MP: Spatially and functionally distinct Ca2+ stores in sarcoplasmic and endoplasmic reticulum. Science 275: 1643–1648, 1997
Ghosh SK, Chakraborti T, Michael JR, Chakraborti S: Oxidant-mediated proteolytic activation of Ca2+ATPase in microsomes of pulmonary smooth muscle. FEBS Lett 387: 171–174, 1996
Piper HM, Siegmund B, Ladilov YV, Schluter KD: Calcium and sodium control in hypoxic reoxygenated cardiomyocytes. Basic Res Cardiol 88: 471–482, 1993
Kimura J, Watanabe Y, Li L, Watano T: Pharmacology of Na+/Ca2+ exchanger. Ann NY Acad Sci 976: 513–519, 2002
Li JM, Kimura J: Translocation mechanism of cardiac Na-Ca exchange. Ann NY Acad Sci 639: 48–60, 1991
Li JM, Kimura J: Translocation mechanism of Na-Ca exchange in single cardiac cells of guinea pig. J Gen Physiol 96: 777–788, 1990
Barry WH, Rasmussen CAF Jr, Ishida H, Bridge JHB: External Na+ independent Ca2+ extrusion in cultured ventricular cells. Magnitude and functional significance. J Gen Physiol 88: 393–411, 1986
Dhalla NS, Panagia V, Singhal PK, Makino M, Dixon IMC, Eyolfson DA: Alterations in heart membrane calcium transport during the development of ischemic-reperfusion injury. J Mol Cell Cardiol 20 (Suppl 2): 3–13, 1988
Reeves JP, Bailey CA, Hale CC: Redox modifications of sodium calcium exchange activity in cardiac sarcolemmal vesicles. J Biol Chem 259: 7733–7739, 1984
Halliwell B, Gutteridge JMC: Role of free radicals and catalytic metal ions in human disease: An overview. Methods Enzymol 186: 1–86, 1990
Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BN, Saul RL, McCord JM, Harman D: Oxygen radicals and human disease. Ann Intern Med 107: 526–545, 1987
Fantone JC, Ward PA: Role of oxygen derived free radicals and metabolites in leukocyte dependent inflammatory reactions. Am J Pathol 107: 395–418, 1982
Burghuber OC, Strife RJ, Zirrolli J, Henson PM, Henson JE, Mathias MM, Reeves JT, Murphy RC, Voelkel NF: Leukotriene inhibitors attenuate rat lung injury induced by hydrogen peroxide. Am Rev Respir Dis 131: 778–785, 1985
Seeger W, Wolf H, Moser V, Neuhof H, Roka L: Influence of aprotinin and gabexate mesylate on arachidonic acid release by the calcium ionophore A23187 in the lung. Adv Exp Med Biol 156: 553–567, 1983
Ramchandran R, Kasturi S, Douglas JG, Sen I: Metalloprotease-mediated cleavage secretion of pulmonary ACE by vascular endothelial and kidney epithelial cells. Am J Physiol 271: H744–H751, 1996
Fernandez-Patron C, Zonki C, Whitta R, Chan JS, Davidge ST, Filef JG: Matrix metalloproteinases regulate neutrophil endothelial cell adhesion through generation of endothelin-1. FASEB J 15: 2230–2240, 2001
Chen M, Zhang Z, Tawiah Boateng MA, Hardwicke PM: A Ca2+ dependent tryptic cleavage site and a protein kinase A phosphorylation site are present in the Ca2+ regulatory domain of scallop muscle Na+/Ca2+ exchanger. J Biol Chem 275: 22961–22968, 2000
Lehotsky J, Kaplan P, Makjovicova M, Murin R, Recay P, Racymaekers L: Ion transport systems as targets of free radicals during ischemia-reperfusion injury. Gen Physiol Biophys 21: 31–37, 2002
Bond JS, Butler PE: Intracellular proteases. Ann Rev Biochem 56: 333–364, 1987
Melligren RL, Meriele MT, Lane RD: Proteolysis of the calcium dependent protease inhibitor by myocardial calcium dependent protease. Arch Biochem Biophys 246: 233–239, 1980
Farrukh IS, Michael JR, Summers W, Adkinson NF, Gurtner GH: Oxidant-mediated pulmonary vasoconstriction: Involvement of calcium. J Appl Physiol 58: 34–44, 1985
Chakraborti T, Ghosh SK, Michael JR, Chakraborti S: Role of aprotinin sensitive protease in the stimulation of Ca2+ATPase by superoxide radical in microsomes of pulmonary smooth muscle. Biochem J 317: 885–890, 1996
Baudhuin P: Morphometry of subcellular fractions. In: Methods Enzymology, Vol 32 (Part B), Academic Press, New York, 1974, pp 3–20
Karmakar S, Ghosh AN, Dey D, Nair GB, Ganguly U: Ultrastructural changes in He La cells associated with enteroadherent Escherichia Coli isolated from infants with diarrhoea in Calcutta. J Diarrhoeal Dis Res 12: 274–278, 1994
Towbin H, Staehlin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci (USA) 76: 4350–4354, 1979
Billings PC, Habres JM, Liao DC, Tuttle SW: Human fibroblasts contain a proteolytic activity which is inhibited by the Bowman-Burk protease inhibitor. Cancer Res 51: 5539–5543, 1991
Brown PD, Kleiner DE, Unsworth EJ, Stetler-Stevenson WG: Cellular activation of the 72 kDa type IV procollagenase/TIMP-2 complex. Kidney Int 43: 163–170, 1993
Dixon IMC, Kaneko M, Hata T, Panagia V, Dhalla NS: Alterations in cardiac membrane calcium transport during oxidative stress. Mol Cell Biochem 99: 125–133, 1990
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with folin-phenol reagent. J Biol Chem 193: 265–275, 1951
David WW: Biostatistics: A foundation for analysis in health science, Wiley, New York, 1978, p. 219
Bellavite P, Corso F, Dusi S, Grzeskowiak M, Della-Bianca V, Rossi F: Activation of NADPH-dependent superoxide production in plasma membrane extracts of pig neutrophils by phosphatidic acid. J Biol Chem 263: 8210–8214, 1988
Patterson CE, Jin N, Packer CS, Rhoades RA: Activated neutrophils alter contractile properties of the pulmonary artery. Am J Respir Cell Mol Biol 6: 260–269, 1992
Phan SH, Gannon DE, Ward PA, Karmiol S: Mechanism of neutrophil-induced xanthine dehydrogenase to xanthine oxidase conversion in endothelial cells: Evidence of a role for elastase. Am J Respir Cell Mol Biol 6: 270–278, 1992
Kettle AJ, Winterbourn CC: Mechanism of inhibition of myeloperoxidase by anti-inflammatory drugs. Biochem Pharmacol 41: 1485–1492, 1991
Shappell SB, Toman C, Anderson DC, Taylor AA, Entman ML, Smith CW: Mac-1 (CD11b/CD18) mediates adherence-dependent hydrogen peroxide production by human and canine neutrophils. J Immunol 144: 2702–2711, 1990
Grisham MB, Gaginella TS, von Ritter C, Tamai H, Be RM, Granger DN: Effects of neutrophil-derived oxidants on intestinal permeability, electrolyte transport, and epithelial cell viability. Inflammation 14: 531–542, 1990
Entman ML, Youker K, Shoji T, Kukielka G, Shappell SB, Taylor AA, Smith CW: Neutrophil induced oxidative injury of cardiac myocytes. A compartmented system requiring CD11b/CD18-ICAM-1 adherence. J Clin Invest 90: 1335–1345, 1992
Suzuki M, Asako H, Kubes P, Jennings S, Grisham MB, Granger DN: Neutrophil-derived oxidants promote leukocyte adherence in postcapillary venules. Microvasc Res 42: 125–138, 1991
Goldhaber JL: Free radicals enhance Na/Ca exchange in ventricular myocytes. Am J Physiol 271: H823–H833, 1996
Chakraborti S, Michael JR: Involvement of serine esterase in the oxidant-mediated activation of phospholipase A2 in pulmonary endothelium. FEBS Lett 281: 185–187, 1991
Doan TN, Gentry DL, Taylor A, Elliott SJ: Hydrogen peroxide activates agonist stimulated Ca2+ flux pathways in canine venous endothelial cells. Biochem J 297: 209–215, 1994
Lewis MS, Whatley RE, Cain P, McIntyre TM, Prescott SM, Zimmerman GA: Hydrogen peroxide stimulates the synthesis of platelet-activating factor by endothelium and induces endothelial cell-dependent neutrophil adhesion. J Clin Invest 82: 2045–2055, 1988
Murphy G, Gavrilovic J: Proteolysis and cell migration: Creating a path? Curr Opin Cell Biol 11: 614–621, 1999
Frisdal E, Gest V, Vieeillard-Baron A, Levame M, Lepilit H, Eddahibi S, Lafuma C, Harf A, Adnot S, Dortho MP: Gelatinase expression in pulmonary arteries during experimental pulmonary hypertension. Eur Respir J 18: 838–845, 2001
Ben-Yosef Y, Lahat N, Shapiro S, Bitterman H, Miller A: Regulation of endothelial matrix metalloproteinase-2 by hypoxia/reoxygenation. Circ Res 90: 784–791, 2002
Hook VYH, Azaryan AV, Hwang SR, Tezapsidis N: Proteases and the emerging role of protease inhibitors in prohormone processing. FASEB J 8: 1269–1278, 1994
Michael JR, Yang J, Farrukh IS, Gurtner GH: Protein kinase C-mediated pulmonary vasoconstriction in rabbit: Role of Ca2+, AA metabolites and vasodilators. J Appl Physiol 74: 1310–1319, 1993
Farrukh IS, Michael JR, Peters SP, Sciuto AM, Adkinson NFJ, Freeland HS, Paky A, Spannhake EW, Summer WR, Gurtner GH: The role of cyclooxygenase and lipoxygenase mediators in oxidant induced lung injury. Am Rev Respir Dis 137: 1343–1350, 1988
White RP: Pharmacodynamic effects of tosyl arginine methylester (TAME) on isolated human arteries. J Gen Physiol 19: 387–392, 1988
Lee KM, Tsai KY, Wang N, Ingber DE: Extracellular matrix and pulmonary hypertension: Control of vascular smooth muscle cell contractility. Am J Physiol 274: H76–H82, 1998
Libby P: Molecular basis of acute coronary syndrome. Circulation 91: 2844–2850, 1995
Ross R: Atherosclerosis: An inflammatory disease. N Engl J Med 340: 115–126, 1999
Shabani F, McNeil J, Tippett L: The oxidative inactivation of tissue inhibitor of metalloproteinase-1 by hypochlorous acid (HOCl) is suppressed by anti-rheumatic drugs. Free Radic Res 28: 115–123, 1998
Yin D, Kuczera K, Squier TC: The sensitivity of carboxyl-terminal methionines in calmodulin isoforms to oxidation by H2O2 modulates the ability to activate the plasma membrane ATPase. Chem Res Toxicol 13: 103–110, 2000
Qu Y, Torchia J, Sen AK: Protein kinase C-mediated activation and phosphorylation of Ca2+ pump in cardiac sarcolemma. Can J Physiol Pharmacol 70: 1230–1235, 1992
Neyses L, Reinlib L, Carafoli E: Phosphorylation of the Ca2+ pumping ATPase of heart sarcolemma and erythrocyte plasma membrane by the cAMP dependent protein kinase. J Biol Chem 260: 10283–10287, 1985
Yoshida Y, Toyosato A, Islam MU, Koga T, Fujita S, Imai S: Stimulation of plasma membrane Ca2+ pump ATPase of vascular smooth muscle by cGMP dependent protein kinase. Functional reconstitution with purified proteins. Mol Cell Biochem 190: 157–167, 1999
Thomas NJ, Carcillo JA, Herzer WA, Mi Z, Tofovic SP, Jackson EK: Type IV phosphodiesterase inhibition improves cardiac contractility in endotoxemic rats. Eur J Pharmacol 465: 133–139, 2003
Murray F, Maclean MR, Pyne NJ: Increased expression of the cGMP inhibited cAMP specific PDE3 and cGMP binding cGMP specific PDE5 phosphodiesterase in models of pulmonary hypertension. Br J Pharmacol 137: 1187–1194, 2002
Schmidt U, Hajjar RJ, Kim CS, Lebeche D, Doye AA, Gwathmey JK: Human heart failure: cAMP stimulation of SR Ca2+ATPase activity and phosphorylation level of phospholamban. Am J Physiol 277: H 474–H480, 1999
Xu A, Narayanan N: Ca2+-calmodulin dependent phosphorylation of the Ca2+ATPase, uncoupled from phospholamban stimulates Ca2+ pump in native cardiac sarcoplasmic reticulum. Biochim Biophys Res Commun 258: 66–72, 1999
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mandal, A., Chakraborti, T., Choudhury, R. et al. Role of MMP-2 in inhibiting Na+ dependent Ca2+ uptake by H2O2 in microsomes isolated from pulmonary smooth muscle. Mol Cell Biochem 270, 79–87 (2005). https://doi.org/10.1007/s11010-005-5260-9
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11010-005-5260-9