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Pro-Inflammatory Cytokine Responses of A549 Epithelial Cells to Antimicrobial Peptide Brevinin-2R

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Abstract

Brevinin-2R is an antimicrobial peptide which has been isolated from the skin of the frog Rana ridibunda. The purpose of the present study was to examine the cellular cytotoxicity and inflammatory effects of brevinin-2R (B2R) on human lung epithelial adenocarcinoma cell line A549. The effects of different concentrations (5, 10, and 20 μg/ml) of B2R on the expression levels of pro-inflammatory cytokines such as IL-1β, and IL-8 in A549 cells were evaluated by semi-quantitative RT-PCR and real-time PCR assays in a dose- and time-dependent manner. Based on the results of MTT assay, B2R showed a moderate cytotoxicity effect in a dose-dependent manner up to 20 % suppression of the cell growth. Moreover, gene expression results demonstrated that B2R up-regulates the IL-1β and IL-8 expression levels in A549 cells in a dose- and time-dependent manner. Our results suggested that brevinin-2R antimicrobial peptide has potentially a regulatory effect on triggering the inflammatory processes.

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Acknowledgments

This work was funded by Grant number 87020301 from the Iran National Science Foundation (INSF) and is gratefully acknowledged by the authors.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

    Ahmad Asoodeh & Reyhane Kashef

  2. Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

    Alireza Haghparast

  3. Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

    Ahmad Asoodeh & Alireza Haghparast

  4. Department of Biology, Faculty of Sciences, Mashhad-Branch, Islamic Azad University, Mashhad, Iran

    Jamshidkhan Chamani

Authors
  1. Ahmad Asoodeh
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  2. Alireza Haghparast
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  3. Reyhane Kashef
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  4. Jamshidkhan Chamani
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Correspondence to Ahmad Asoodeh.

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Asoodeh, A., Haghparast, A., Kashef, R. et al. Pro-Inflammatory Cytokine Responses of A549 Epithelial Cells to Antimicrobial Peptide Brevinin-2R. Int J Pept Res Ther 19, 157–162 (2013). https://doi.org/10.1007/s10989-012-9328-6

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  • DOI: https://doi.org/10.1007/s10989-012-9328-6

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