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The Chemical Synthesis of the Collagenous Domain of the Hormone Adiponectin

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Abstract

As a first step towards the preparation of a functional biomolecule, a chemical synthesis of the collagenous domain of adiponectin is described. The 76 residue polypeptide (without post-translational modifications) could be assembled efficiently from smaller unprotected peptides using two native chemical ligation reactions followed by a reductive desulfurisation step. In turn, the polypeptides were synthesised in high purity by microwave enhanced solid phase synthesis.

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Acknowledgments

The authors wish to thank The Maurice Wilkins Centre for Molecular Biodiscovery for financial support.

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Authors and Affiliations

  1. School of Chemical Sciences and Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, 23 Symonds St, Auckland, 1142, New Zealand

    Paul W. R. Harris & Margaret A. Brimble

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  1. Paul W. R. Harris
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  2. Margaret A. Brimble
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Correspondence to Paul W. R. Harris.

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Harris, P.W.R., Brimble, M.A. The Chemical Synthesis of the Collagenous Domain of the Hormone Adiponectin. Int J Pept Res Ther 18, 63–70 (2012). https://doi.org/10.1007/s10989-011-9279-3

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  • DOI: https://doi.org/10.1007/s10989-011-9279-3

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