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ChBac3.4: A Novel Proline-Rich Antimicrobial Peptide from Goat Leukocytes

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An Erratum to this article was published on 21 February 2009

Abstract

We isolated a new proline-rich peptide, ChBac3.4, from leukocytes of the goat (Capra hirca) and determined its amino acid sequence by Edman degradation and mass spectrometry. ChBac3.4 (RFRLPFRRPPIRIHPPPFYPPFRRFL–NH2) had over 50% sequence identity to the Bac5 peptides found in the leukocytes of goats, sheep and cattle. ChBac3.4 exhibited broadspectrum antimicrobial activity, especially under low salt conditions. Since E. coli ML35p treated with ChBac3.4 manifested increased outer and inner membrane permeability and a rapid and extensive loss of cytoplasmic potassium, the antimicrobial properties of this peptide may depend, in part, on its ability to damage microbial membranes. Nevertheless, even high concentrations of ChBac3.4 were not significantly hemolytic for human erythrocytes. In vitro, ChBac3.4 was selectively cytotoxic, damaging human K562 erythroleukemia cells and human U937 hystiocytic lymphoma cells, but not other human target cells. ChBac3.4 appears to differ from other proline-rich cathelicidins in virtue of its increased ability to damage microbial membranes. This novel antimicrobial peptide warrants further study, especially with respect to its various effects on microbial and mammalian cells.

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Abbreviations

AU:

Acid-urea

CEE:

Continuous elution electrophoresis

MHB:

Mueller-Hinton broth

MIC:

Minimal inhibitory concentration

PAGE:

Polyacrylamide gel electrophoresis

PBS:

Phosphate buffered saline

TSB:

Tryptic soy broth

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Acknowledgments

This work was supported by INTAS Grant (Ref. No. 03-51-4984), Russian Foundation of Basic Research (No. 07-04-01759; No. 06-04-49416), by the BONFOR programme of the University of Bonn, FVG regional Grant 200502027001 and the European Fond for Regional Structure Development (EFRE, European Union and Free State Saxony). We acknowledge The Center of United Users “Analytical Spectrometry” at St-Petersburg State Polytechnic University for providing the opportunity to use equipment belonging to The Center in the course of our research.

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Authors and Affiliations

  1. Department of General Pathology and Pathophysiology, Institute for Experimental Medicine of the Russian Academy of Medical Sciences, St-Petersburg, Russia

    Olga Shamova, Dmitriy Orlov & Vladimir Kokryakov

  2. Department of Chemistry and Mineralogy, Institute of Bioanalytical Chemistry, Leipzig University, Leipzig, Germany

    Christin Stegemann, Patricia Czihal & Ralf Hoffmann

  3. Department of Periodontics, Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City, IA, USA

    Kim Brogden

  4. Research Institute of Pure Biochemicals, St-Petersburg, Russia

    Nikolay Kolodkin

  5. Institute of Cytology of the Russian Academy of Science, St-Petersburg, Russia

    Galina Sakuta

  6. Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Trieste, Italy

    Alessandro Tossi

  7. Institute of Medical Microbiology, Immunology and Parasitology – Pharmaceutical Microbiology Section, University of Bonn, Bonn, Germany

    Hans-Georg Sahl

  8. David Geffen School of Medicine at University of California, Los Angeles, CA, USA

    Robert I. Lehrer

Authors
  1. Olga Shamova
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  11. Vladimir Kokryakov
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  12. Robert I. Lehrer
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Correspondence to Olga Shamova.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10989-009-9170-7

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Shamova, O., Orlov, D., Stegemann, C. et al. ChBac3.4: A Novel Proline-Rich Antimicrobial Peptide from Goat Leukocytes. Int J Pept Res Ther 15, 31–42 (2009). https://doi.org/10.1007/s10989-008-9159-7

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  • DOI: https://doi.org/10.1007/s10989-008-9159-7

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