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Thermal analysis in biological and medical applications

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Abstract

In this paper, I will sum up our research activity from this field performed in the last about 25 years. I will focus on three main points: basic muscle research in the different intermediate states of ATP hydrolysis cycle during muscle contraction, R&D activities to develop and test different dairy products and TA application in some surgical and diagnostic problem. Our initial research concerned the investigation of thermal stability of main muscle proteins clarifies their basic unfolding characteristics. Later we extended the thermal stability investigation from protein solution to the myosin myofibrils and to the higher organization of muscle proteins, the muscle fibres, checking the effect of nucleotides. At that time, it became possible to stabilize the different intermediate states of ATP hydrolysis up to the time of DSC measurement, using different Pi analogues (Vi, AlFx and BeFx) and non-hydrolysable ATP analogue (AMP.PNP). This way the targets were AM.ADP.Vi (and with AlFx or BeFx) so-called weak binding state, AM.ADP the “strong” binding state as well as the “rigor” AM complex state. AM.AMP.PNP state was used to mimic the AM.ATP state. With our R&D cooperation, a cold spreadable butter was successfully developed. We were a partner in the development of Ca-enriched cheese, in its spreadable form too as well as in the development and testing of different dairy products using probiotic cultures. Our TA activity covers a wide range of medical applications, e.g. investigation of the different abnormalities of human leg skeletal muscle, different stages of degeneration of human vertebral disc, to judge the goodness/applicability of different suture technique on tracheal cartilage in primary airway reconstruction or the characterization of different self-expandable stents implantation in the oesophagus treatment. We have joined those groups who try to use DSC in the diagnosis of different diseases from blood plasma, e.g. in the case of breast cancer, melanoma, in psoriasis.

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Acknowledgements

The SETARAM Micro DSC-II (Caluire, France) calorimeter was supported by grant from the Hungarian Scientific Research Found (NKFIH) CO-272. All the figures, tables and results are involved in this paper by the permission of JTAC/Akadémiai Kiadó-Springer Nature (Hungary). The paper based on the 5th SETARAM ICTAC Award (Orlando, USA 2016) sponsored by SETARAM and is dedicated to the 650th anniversary of the foundation of the University of Pécs, Hungary.

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  1. Department of Biophysics, School of Medicine, University of Pécs, Szigeti Str. 12, Pécs, 7624, Hungary

    D. Lőrinczy

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  1. D. Lőrinczy
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Correspondence to D. Lőrinczy.

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The paper is based on the ICTAC-2016 SETARAM Award plenary lecture.

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Lőrinczy, D. Thermal analysis in biological and medical applications. J Therm Anal Calorim 130, 1263–1280 (2017). https://doi.org/10.1007/s10973-017-6308-2

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