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Harmful effect of neoadjuvant chemotherapy monitoring by DSC on breast cancer patients’ blood plasma

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Abstract

Preoperative neoadjuvant chemotherapy is currently a common approach for treatment of breast cancer patients. These anticancer drugs are harmful, but essential in the fight against tumor cells. The purpose of this pilot study was to compare the thermal changes of blood plasma with differential scanning calorimetry (DSC) method between neoadjuvant chemotherapy received and not received breast cancer patients. The study included 11 adult breast cancer women who were grouped according to TNM stage and perioperative treatments. Patients who had ductal carcinoma in situ (DCIS), as a local tumor without any metastases did not receive any neoadjuvant chemotherapy (n = 3). Patients with invasive lobular carcinoma with local metastases received neoadjuvant chemotherapy preoperatively (n = 8). Peripheral blood samples were collected from the patients before the operation and from healthy female controls (n = 5) ranging from 26 to 60 years old. Denaturation of plasma components was detected in SETARAM Micro DSC-II calorimeter. Our results showed in control group two main T ms (~56 and 63 °C as averages) with ΔH ~ 1.16 J g−1. In DCIS samples three T ms (~55.5; 60.6 and 65.5 °C) with ΔH ~ 1.01 J g−1, while in group with neoadjuvant chemotherapy four T ms (56.3; 60.9; 66.3 and 74.4 °C) with ΔH = 1.07 J g−1 were observed in average. These measurements demonstrated that neoadjuvant chemotherapy aggravates the thermodynamic changes in the blood plasma samples of invasive breast cancer patients.

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References

  1. Senkus E, Kyriakides S, Ohno S, Penault-Llorca F, Poortmans P, Rutgers E, Zackrisson S, Cardoso F. Primary breast cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2015;26:8–30.

    Article  Google Scholar 

  2. Matsen CB, Neumayer LA. Breast cancer: a review for the general surgeon. JAMA Surg. 2013;148:971–9.

    Article  Google Scholar 

  3. Wuttke M, Phillips KA. Clinical management of women at high risk of breast cancer. Curr Opin Obstet Gynecol. 2015;27:6–13.

    Article  Google Scholar 

  4. Masarwah A, Auvinen P, Sudah M, Rautiainen S, Sutela A, Pelkonen O, Oikari S, Kosma V-M, Vanninen R. Very low mammographic breast density predicts poorer outcome in patients with invasive breast cancer. Eur Radiol. 2015;25:1875–82.

    Article  Google Scholar 

  5. Van Cleef A, Altintas S, Huizing M, Papadimitriou K, Van Dam P, Tjalma W. Current view on ductal carcinoma in situ and importance of the margin thresholds: a review. Facts Views Vis Obgyn. 2014;6:210–8.

    Google Scholar 

  6. Wyld L, Audisio RA, Poston GJ. The evolution of cancer surgery and future perspectives. Nat Rev Clin Oncol. 2015;12:115–24.

    Article  Google Scholar 

  7. Thompson AM, Moulder-Thompson SL. Neoadjuvant treatment of breast cancer. Ann Oncol. 2012;23:231–6.

    Article  Google Scholar 

  8. Anderson NL, Anderson NG. The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics. 2002;1:845–67.

    Article  CAS  Google Scholar 

  9. Garbett NC, Miller JJ, Jenson AB, Chaires JB. Calorimetric analysis of the plasma proteome. Semin Nephrol. 2007;27:621–6.

    Article  CAS  Google Scholar 

  10. Fekecs T, Zapf I, Ferencz A, Lőrinczy D. Differential scanning calorimetry (DSC) analysis of human plasma in melanoma patients with or without regional lymph node metastases. J Therm Anal Calorim. 2012;108:149–52.

    Article  CAS  Google Scholar 

  11. Todinova S, Krumova S, Gartcheva L, Robeerst C, Taneva SG. Microcalorimetry of blood serum proteome: a modified interaction network in the multiple myeloma case. Anal Chem. 2011;83:7992–8.

    Article  CAS  Google Scholar 

  12. Chagovetz AA, Quinn C, Damarse N, Hansen LD, Chagovetz AM, Jensen RL. Differential scanning calorimetry of gliomas: a new tool in brain cancer diagnostics? Neurosurgery. 2013;73:289–95.

    Article  Google Scholar 

  13. Garbett NC, Mekmaysy CS, DeLeeuw L, Chaires JB. Clinical application of plasma thermograms: utility, practical approaches and considerations. Methods. 2015;76:41–50.

    Article  CAS  Google Scholar 

  14. Vega S, Garcia-Gonzalez MA, Lanas A, Velazquez-Campoy A, Abian A. Deconvolution analysis for classifying gastric adenocarcinoma patients based on differential scanning calorimetry serum thermograms. Sci Rep. 2015;5:7988.

    Article  CAS  Google Scholar 

  15. Todinova S, Krumova S, Kurtev P, Dimitrov V, Djongov L, Dudunkov Z, Taneva SG. Calorimetry-based profiling of blood plasma from colorectal cancer patients. Biochim Biophys Acta. 2012;1820:1879–85.

    Article  CAS  Google Scholar 

  16. Fish DJ, Brewood GP, Kim JS, Garbett NC, Chaires JB, Benight AS. Statistical analysis of plasma thermograms measured by differential scanning calorimetry. Biophys Chem. 2010;152:184–90.

    Article  CAS  Google Scholar 

  17. Garbett NC, Merchant ML, Helm CW, Jenson AB, Klein JB, Chaires JB. Detection of cervical cancer biomarker patterns in blood plasma and urine by differential scanning calorimetry and mass spectrometry. PLoS One. 2014;9:e84710.

    Article  Google Scholar 

  18. Zapf I, Fekecs T, Ferencz A, Tizedes GY, Pavlovics G, Kalmán E, Lőrinczy D. DSC analysis of human plasma in breast cancer patients. Thermochim Acta. 2011;524:88–91.

    Article  CAS  Google Scholar 

  19. Zapf I, Moezzi M, Fekecs T, Nedvig K, Lőrinczy D, Ferencz A. Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients. J Therm Anal Calorim. 2015. doi:10.1007/s10973-015-4642-9.

    Google Scholar 

  20. Kikalishvili L, Ramishvili M, Nemsadze G, Lezhava T, Khorava P, Gorgoshidze M, Kiladze M, Monaselidze J. Thermal stability of blood plasma proteins of breast cancer patients, DSC study. J Therm Anal Calorim. 2015;120:501–5.

    Article  CAS  Google Scholar 

  21. Fatima T, Roohi N, Abid R. Circulatory proteins in women with breast cancer and their chemotherapeutic responses. Pak J Zool. 2013;45:1207–13.

    CAS  Google Scholar 

  22. Moezzi M, Ferencz A, Lőrinczy D. Evaluation of blood plasma changes by differential scanning calorimetry in psoriatic patients treated with drugs. J Therm Anal Calorim. 2014;116:557–62.

    Article  CAS  Google Scholar 

  23. Könczöl F, Wiegand N, Nőt LG, Lőrinczy D. Examination of the cyclophosphamide induced polyneuropathy on guinea pig sciatic nerve and gastrocnemius muscle with differential scanning calorimetry. J Therm Anal Calorim. 2014;115:2239–43.

    Article  Google Scholar 

  24. Michnik A, Polaczek-Grelik K, Leśniak P, Drzazga Z. Effects of low-dose ionizing radiation on α, β-globulins solutions studied by DSC. J Therm Anal Calorim. 2013;111:1845–52.

    Article  CAS  Google Scholar 

  25. Michnik A, Drzazga Z. Thermal denaturation of mixtures of human serum proteins, DSC study. J Therm Anal Calorim. 2010;101:513–8.

    Article  CAS  Google Scholar 

  26. Chu HL, Chen TH, Wu CY, Yang YC, Tseng SH, Cheng TM, Ho LP, Tsai LY, Li HY, Chang CS, Chang CC. Thermal stability and folding kinetics analysis of disordered protein, securin. J Therm Anal Calorim. 2014;115:2171–8.

    Article  CAS  Google Scholar 

  27. Moezzi M, Zapf I, Fekecs T, Nedvig K, Lőrinczy D, Ferencz A. Influence of oxidative injury and monitoring of blood plasma by DSC on patients with psoriasis. J Therm Anal Calorim. 2015. doi:10.1007/s10973-015-4674-1.

    Google Scholar 

  28. Splinter R, van Herwaarden AW, van Wetten IA, Pfreundt A, Svendsen WE. Fast differential scanning calorimetry of liquid samples with chips. Thermochim Acta. 2014;603:162–71.

    Article  Google Scholar 

  29. Arias-Moreno X, Abian O, Vega S, Sancho J, Velazquez-Campoy A. Protein–cation interactions: structural and thermodynamic aspects. Curr Protein Pept Sci. 2011;12:325–38.

    Article  CAS  Google Scholar 

  30. Garbett NC, Chaires JB. Thermodynamic studies for drug design and screening. Expert Opin Drug Discov. 2012;7:299–314.

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by Grants OTKA CO-272 (for Dénes Lőrinczy). The present scientific contribution is dedicated to the 650th anniversary of the foundation of the University of Pécs, Hungary.

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Authors and Affiliations

  1. Department of Surgical Research and Techniques, Medical Faculty, Semmelweis University, Nagyvárad Square 4, Budapest, 1089, Hungary

    A. Ferencz

  2. Surgery Clinic, Medical School, University of Pécs, Ifjúság Street 13, Pecs, 7624, Hungary

    I. Zapf

  3. Institute of Biophysics, School of Medicine, University of Pécs, Szigeti Street 12, Pecs, 7624, Hungary

    D. Lőrinczy

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  1. A. Ferencz
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  2. I. Zapf
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  3. D. Lőrinczy
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Correspondence to A. Ferencz or D. Lőrinczy.

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Ferencz, A., Zapf, I. & Lőrinczy, D. Harmful effect of neoadjuvant chemotherapy monitoring by DSC on breast cancer patients’ blood plasma. J Therm Anal Calorim 126, 55–59 (2016). https://doi.org/10.1007/s10973-016-5291-3

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  • DOI: https://doi.org/10.1007/s10973-016-5291-3

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