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Thermal degradation, kinetic analysis, and apoptosis induction in human melanoma for oleanolic and ursolic acids

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Abstract

This study deals with the comparative analysis of thermal degradation (including kinetic study) in dynamic air atmosphere, for two similar-structured triterpenoid acids—ursolic (URS) and oleanolic (OLE) acids. As kinetic methods, two integral methods (Kissinger–Akahira–Sunose and Flynn–Wall–Ozawa) and one differential method (Friedman) were used. The “in-depth” kinetic analysis was completed by applying the nonparametric kinetics (NPK) method, which confirmed the multiprocess degradation suggested by isoconversional methods (the observed variation in E a vs. conversion degree). The NPK method revealed that ursolic acid is degraded solely by two parallel chemical processes, while in the case of oleanolic acid, the processes are accompanied by physical ones. The physicochemical investigation was completed with a bioactivity evaluation regarding the apoptotic process caused by the two compounds in A2058 human melanoma cell line. It was found that the similar-structured triterpenoids possess different biological effects, OLE being antiapoptotic, while URS being strong apoptotic.

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Acknowledgements

This work was supported by a grant from the University of Medicine and Pharmacy “Victor Babeş” Timişoara (Grant III-C1-PCFI-2014/2015-03 SYNTANTITUM to AF, IL, CS, CO, LMS, and SA).

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Authors and Affiliations

  1. Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041, Timisoara, Romania

    Adriana Fuliaş, Ionuţ Ledeţi, Codruţa Şoica, Cristina Dehelean, Camelia Oprean, Lenuţa-Maria Şuta & Ştefana Avram

  2. Research Center for Thermal Analysis in Environmental Problems, Faculty of Chemistry-Biology-Geography, West University of Timisoara, Pestalozzi Street 16, 300115, Timisoara, Romania

    Gabriela Vlase & Titus Vlase

  3. Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041, Timisoara, Romania

    Florina Bojin

  4. Faculty of Industrial Chemistry and Environmental Engineering, Politehnica University Timişoara, 6 Carol Telbisz, 300001, Timisoara, Romania

    Vasile Bercean

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  1. Adriana Fuliaş
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  2. Ionuţ Ledeţi
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  3. Gabriela Vlase
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Correspondence to Ionuţ Ledeţi.

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Fuliaş, A., Ledeţi, I., Vlase, G. et al. Thermal degradation, kinetic analysis, and apoptosis induction in human melanoma for oleanolic and ursolic acids. J Therm Anal Calorim 125, 759–768 (2016). https://doi.org/10.1007/s10973-015-5052-8

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  • DOI: https://doi.org/10.1007/s10973-015-5052-8

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