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Comprehensive evaluation of solution nuclear magnetic resonance spectroscopy sample preparation for helical integral membrane proteins

  • Original Paper
  • Published:
Journal of Structural and Functional Genomics

Abstract

The preparation of high quality samples is a critical challenge for the structural characterization of helical integral membrane proteins. Solving the structures of this diverse class of proteins by solution nuclear magnetic resonance spectroscopy (NMR) requires that well-resolved 2D 1H/15N chemical shift correlation spectra be obtained. Acquiring these spectra demands the production of samples with high levels of purity and excellent homogeneity throughout the sample. In addition, high yields of isotopically enriched protein and efficient purification protocols are required. We describe two robust sample preparation methods for preparing high quality, homogeneous samples of helical integral membrane proteins. These sample preparation protocols have been combined with screens for detergents and sample conditions leading to the efficient production of samples suitable for solution NMR spectroscopy. We have examined 18 helical integral membrane proteins, ranging in size from approximately 9 kDa to 29 kDa with 1–4 transmembrane helices, originating from a number of bacterial and viral genomes. 2D 1H/15N chemical shift correlation spectra acquired for each protein demonstrate well-resolved resonances, and >90% detection of the predicted resonances. These results indicate that with proper sample preparation, high quality solution NMR spectra of helical integral membrane proteins can be obtained greatly enhancing the probability for structural characterization of these important proteins.

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Acknowledgements

The authors thank T.M. Logan of Florida State University for his helpful discussion concerning initial solution NMR spectroscopy of helical IMPs from M. tuberculosis. The authors also thank B. Xu, D.H. Jones, and S.H. Park of University of California, San Diego, and H. Qin and Y. Hua of Florida State University for their contributions to the work. This work was supported by NIH grant PO1 GM064676 and NSF grant MCB-0235774. A portion of this work was performed at the National High Magnetic Field Laboratory funded by the National Science Foundation (DMR 0084173) and the State of Florida. Parts of this research were performed in the Environmental Molecular Sciences Laboratory (a national scientific user facility sponsored by the U.S. DOE Office of Biological and Environmental Research) located at Pacific Northwest National Laboratory, operated by Battelle for the DOE. This research benefited from activities at the Southeast Collaboratory for High-Field Biomolecular NMR, a research resource at the University of Georgia, funded by the National Institute of General Medical Sciences (NIGMS grant number P41 GM066340) and the Georgia Research Alliance. The research utilized the Biomedical Technology Resource for NMR Molecular Imaging of Proteins supported by NIH grant P41 EB002031. C.K. Mobley was supported by NIH training grant T32GM008320. J.D. Moore was supported by NIH predoctoral fellowship F31NS054494. R.C. Page and H.B. Nguyen were supported by American Heart Association predoctoral fellowships.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida, 32306-4390, USA

    Richard C. Page, Jacob D. Moore, Mukesh Sharma, Rose Chase, Fei Philip Gao & Timothy A. Cross

  2. National High Magnetic Field Laboratory, 1800 E. Paul Dirac Drive, Tallahassee, Florida, 32310, USA

    Richard C. Page, Jacob D. Moore, Hau B. Nguyen, Mukesh Sharma, Rose Chase, Fei Philip Gao & Timothy A. Cross

  3. Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, 32306-4380, USA

    Hau B. Nguyen & Timothy A. Cross

  4. Department of Biochemistry and Center for Structural Biology, Vanderbilt University, Nashville, Tennessee, 37232-8725, USA

    Charles K. Mobley & Charles R. Sanders

  5. Department of Physiology & Biophysics, and the Cleveland Center for Structural Biology, Case Western Reserve University, Cleveland, Ohio, 44106, USA

    Liping Ma & Frank D. Sönnichsen

  6. Department of Chemistry and Biochemistry, University of California, San Diego, CA, 92093-0307, USA

    Sangwon Lee, Stanley C. Howell & Stanley J. Opella

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  1. Richard C. Page
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  2. Jacob D. Moore
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Correspondence to Timothy A. Cross.

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Page, R.C., Moore, J.D., Nguyen, H.B. et al. Comprehensive evaluation of solution nuclear magnetic resonance spectroscopy sample preparation for helical integral membrane proteins. J Struct Funct Genomics 7, 51–64 (2006). https://doi.org/10.1007/s10969-006-9009-9

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  • DOI: https://doi.org/10.1007/s10969-006-9009-9

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