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The effect of radiolabeled antibiotics on biofilm and microorganism within biofilm

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Abstract

The aim of this study was to investigate the 131I and 127I labeled linezolid and moxifloxacin effects of minimum inhibitory concentration, and minimum bactericidal concentration on mature biofilm and microorganism within the biofilm. Linezolid and moxifloxacin were labeled with 131I and 127I and chromatography studies were carried out with thin layer radiochromatograpy and high-pressure liquid radiochromatography techniques. Specific activities of radiolabeled LZD and MXF was found to be 53.3 ± 3.1 and 127.3 ± 1.1 MBq/µmol for [131I]LZD and 7.6 ± 0.02 and 55.6 ± 0.8 MBq/µmol for [131I]MXF, respectively. The minimum inhibitory concentration and Time-Kill of Linezolid and moxifloxacin alone and their 131I and 127I labeled forms were tested with a standard strain of meticillin-susceptible Staphylocıccus aureus. MIC values of LNZ and MXF were 2.96 nmol/mL (1 µg/ml) and 0.141 nmol/mL (0.062 µg/ml). Time Kills of MXF and LZD were found to be 0.06 and 1 μg, respectively. Antibiotics labeled with beta-emitting radioactive molecule may be a new theranostics strategy for biofilm infections.

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Acknowledgements

We are thankful to Dr. F.Gül Gümüşer and Dr. E. Sayit Bilgin (ManisaCelal Bayar University) for providing the laboratory conditions. We also thank Norma Merce Yagcier for reviewing our study as a native Engish speaker.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Arts and Science, Manisa Celal Bayar University, Yunus Emre, Manisa, 45140, Turkey

    Uğur Avcıbaşı, Hasan Demiroğlu & Buket Ateş

  2. Department of Infectious Diseases and Clinical Microbiology, Adnan Menderes University School of Medicine, Aydin, 09010, Turkey

    Serhan Sakarya

  3. Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Bornova, İzmir, 35100, Turkey

    Perihan Ünak & Volkan Tekin

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  1. Uğur Avcıbaşı
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Correspondence to Uğur Avcıbaşı.

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Avcıbaşı, U., Demiroğlu, H., Sakarya, S. et al. The effect of radiolabeled antibiotics on biofilm and microorganism within biofilm. J Radioanal Nucl Chem 316, 275–287 (2018). https://doi.org/10.1007/s10967-018-5750-3

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  • DOI: https://doi.org/10.1007/s10967-018-5750-3

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