Skip to main content
SpringerLink
Log in

Suitable dose of 60Co γ-ray for mutation in Roegneria seeds

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

Seeds from six accessions of three species of Roegneria were radiated with 60Co γ-ray at different doses (50, 100, 150, 200, 250, 300 and 400 Gy). Following these treatments, germination energy, germination rate, seedling height, plant height, plant survival, and seed set were observed. Plant survival was highly correlated with seedling height (R 2 > 0.91, P < 0.01) and seed set (R 2 > 0.82, P < 0.01). The semi-lethal dose of each accession, calculated using a ‘Multi-target single-hit’ model, ranged from 60 to 173 Gy. The most suitable absorbed doses for each accession were deduced from these data. The suitable doses for Roegneria kamoji, Roegneria ciliaris and Roegneria japonensis were 65–100 Gy, 63–150 Gy and 80–170 Gy, respectively. According to the range of suitable doses, R. kamoji (Pr87-88-353) was the most sensitive to radiation, and R. japonensis (88-89-267) was the most resistant to radiation. Suitable doses of R. ciliaris were close to that of R. kamoji (ZY1007). This research provides preliminary guidelines for radiation induced mutagenesis in Roegneria.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Joint FAO/IAEA Programme. http://www-naweb.iaea.org/nafa/pbg/index.html

  2. Su SF (1986) Breeding report of early maturity variety-Astragalus huangheensis. Chin J Grassl 5(1):41–48

    Google Scholar 

  3. Yin HY, Yu HB, Ma JZ (1992) Study on toxicity mutation of crown vetch induced by radiation. Acta Agric Nucl Sin 6(3):129–133

    Google Scholar 

  4. Li H, Luo XY, Wang DK (1996) Breeding of new variety Nongmu no.801 of Medicago sativa L. Heilongjiang Anim Sci Vet Med 5:15–16

    Google Scholar 

  5. Yin SX, Wang YH, Zhou RR (2005) The effect of 60Co γ-ray irradiation on seeds germination and POD isozymes of perennial ryegrass. Grassl China 27(1):75–79

    Google Scholar 

  6. Xu GR (1992) Plant mutation breeding. Chin Agric Press, Beijing

    Google Scholar 

  7. Dickens R, Johnston WJ, Haaland WL (1981) Variability observed in centipedegrass grown from 60Co irradiated seed. Agron J 73:674–6763

    Article  CAS  Google Scholar 

  8. Philip B (1980) Gamma ray dosage and mutation breeding in St. Augustinegrass. Crop Sci 20:181–184

    Article  Google Scholar 

  9. Kang YF, Ma HL, Chen YJ, Shen QH, Hai T (1998) Suitable radiation dose of alfalfa varieties in China. Inn Mong Pratac 1:1–6

    Google Scholar 

  10. Zhi ZS, Gao WH, Zhang EH (1999) The effect of γ-ray radiation on Sorghum sudanense. Inn Mong Pratac 2:36–38

    Google Scholar 

  11. Zhang YQ, Jia WL, Yang LL, Guo XL, Wu Y, Hu YL, Lin ZP (2005) Study on character variation of Festuca arundinacea radiation by 60Co γ-ray. Acta Pratatac Turae Sin 14(4):65–71

    Google Scholar 

  12. Yen C, Yang JL, Baum BR (2006) Biosystematics of Triticeae, vol 3. China Agric Press, Beijing

    Google Scholar 

  13. Lei TS, Zhang QB, Hua YP, Li J, Zhang XZ (1998) Aridupland pasture resource features and evaluation of Roegneria alaschnica in east part of Xinjiang. Grassl China 3:17–21

    Google Scholar 

  14. Wang JL, Zhang YX, Shen ZB, Tang FL, Li DM, Zhang RB, Pan DF, Zhong P (2009) A preliminary study on mutagenic effect of 60Co-γ ray radiation in Roegneria. Grassl Turf 2:10–13

    Google Scholar 

  15. Puck TT, Marcus PI (1956) Action of x-rays on mammalian cells. J Exp Med 103(5):653–666

    Article  CAS  Google Scholar 

  16. Luria SE (1947) Reactivation of irradiated bacteriophage by transfer of self-reproducing units. Proc Natl Acad Sci 33:253–264

    Article  CAS  Google Scholar 

  17. Atwood KC, Norman A (1949) On the interpretation of multi-hit survival curves. Proc Natl Acad Sci 35(12):696–709

    Article  CAS  Google Scholar 

  18. Wang YH, Han LB, Yin SX, Wang JL, Cheng XX (2006) The effect of γ-rays irradiation on seeds germination and enzyme activity of tall fescue. J Nucl Agric Sci 20(3):199–201

    CAS  Google Scholar 

  19. Cai CJ, Gao J, Mu SH (2007) Effects of 60Co γ rays radiation on seed vigor and young seedling growth of Phyllostachys edulis. J Nucl Agric Sci 21(5):436–440

    Google Scholar 

  20. Yao YS, Wang HH, Zhao LZ (1995) Correlation between seedling growth and plant survival study of spring wheat radiated by fast neutron. J Nucl Agric Sci 16(1):9–12

    Google Scholar 

  21. Krishna G, Shivashankar G, Nath J (1984) Mutagenic response of rhodes grass (Chloris gatana Kunth.) to gamma rays. Environ Exp Bot 24(2):197–205

    Article  Google Scholar 

  22. Inoue M, Ito R, Tabata T, Hasegawa H (1980) Varietal differences in the repair of gamma-radiation-induced lesions in barley. Environ Exp Bot 20:161–168

    Article  Google Scholar 

  23. Wang BW, Hao XQ, Wang DJ, Zhao G, Wu ZK (2007) Studies on proper dose irradiated by γ-ray to micro-endosperm super-high oil corn and effects on seedling height. Crop Sci 15(2):137–139

    Google Scholar 

  24. Sachs BR (1965) Stem elongation. Plant Physiol 16:73–96

    Article  CAS  Google Scholar 

  25. Liao FX, Fan RZ (2001) The effects of seeds radiation with 60Co-γ on seed germination and shoot growth of Brassica campetris L. ssp. Chinensis var. utilis. Acta Agric Nucl Sin 15(1):6–10

    Google Scholar 

  26. Sparow AH, Evans HJ (1961) Nuclear factors affecting radio-sensitivity. I. The influence of nuclear size and structure, chromosome complement, and DNA content. Brookhaven Symp Biol 14:76–100

    Google Scholar 

  27. Evans HJ, Sparrow AH (1961) Nuclear factors affecting radio-sensitivity. II. Dependence on nuclear and chromosome structure and organization. Brookhaven Symp Biol 14:101–127

    CAS  Google Scholar 

  28. Davies DR (1962) The genetical control of radiosensitivity. II. Growth measurements in Lycopersicum and Melandrium. Radiat Bot 1:277–2955

    Article  Google Scholar 

  29. Rudolph TD (1971) Gymnosperm seedling sensitivity to gamma radiation: its relation to seed radiosensitivity and nuclear variables. Radiat Bot 11:45–51

    Article  Google Scholar 

  30. Stollov M, Jansson G, Eriksson G, Ehrenberg L (1966) Genetical and physiological causes of the variation of radiosensitivity in barley and maize. Radiat Bot 6:457–467

    Article  Google Scholar 

  31. Takagi Y (1969) The second type of gamma-ray sensitive gene RS2 in soybean Glycine max (L.) Merrill. Gamma Field Syrup 8:83–94

    Google Scholar 

  32. Conger BV, Nilan RA, Konzak CF, Metter S (1966) The influence of seed water content on the oxygen effect in irradiated barley seeds. Radiat Bot 6:129–144

    Article  Google Scholar 

  33. Conger BV, Nilan RA, Konzak CF (1968) Post-irradiation oxygen sensitivity of barley seeds varying slightly in water content. Radiat Bot 8:31–36

    Article  Google Scholar 

  34. Conger BV, Constantin MJ, Carabia JV (1972) Seed radiosensitivity: wide range in oxygen-enhancement ratio after gamma irradiation of eight species. Int J Radiat Biol 22:225–235

    Article  CAS  Google Scholar 

  35. Ma HL, Hai T, Shen QH, Chen YJ, Kang YF (1995) Suitable dosages and radiation sensitivities for 89 forage legumes. Grassl China 2:6–11

    Google Scholar 

Download references

Acknowledgments

We greatly appreciate Luo CL and Chen WJ for assistance with seeding. This research was made possible by support from National Natural Science Foundation of China (Nos. 30870154, 30901052).

Author information

Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, 611130, Sichuan, People’s Republic of China

    Xiaomei Luo, Haiqin Zhang & Yonghong Zhou

  2. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Building, Central Experimental Farm, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada

    Nicholas A. Tinker

  3. Institute of Biological and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, 610061, Sichuan, People’s Republic of China

    Yun Jiang & Pu Xuan

Authors
  1. Xiaomei Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nicholas A. Tinker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yun Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pu Xuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Haiqin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yonghong Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yonghong Zhou.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Luo, X., Tinker, N.A., Jiang, Y. et al. Suitable dose of 60Co γ-ray for mutation in Roegneria seeds. J Radioanal Nucl Chem 295, 1129–1134 (2013). https://doi.org/10.1007/s10967-012-1888-6

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-012-1888-6

Keywords

Search

Navigation