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A radioanalytical technique for measurement of beta-glucuronidase activities

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Summary

Phenolphthalein-glucuronide is a commonly used glucuronide conjugate for beta-glucuronidase measurements. The quantity of phenolphthalein liberated by beta-glucuronidase is measured spectrophotometrically. The detection limit of the quantity of phenolphthalein using spectrophotometry is a few μg. In this study, a new radioanalytical technique for the measurement of beta-glucuronidase was applied which is 106times more sensitive than the spectrophotometric technique. Radioiodinated phenolphthalein-glucuronide and phenyl-N-glucuronide were used in this study in which the beta-glucuronidase levels of some tissue samples were measured.

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Authors and Affiliations

  1. Ege University, Faculty of Science, Department of Chemistry, Division of Nuclear Chemistry, Bornova, Izmir 35100, Turkey

    T. Ünak

  2. Ege University, Faculty of Science, Department of Chemistry, Division of Nuclear Chemistry, Bornova, Izmir 35100, Turkey

    U. Avcibaşi

  3. Ege University, Faculty of Science, Department of Chemistry, Division of Nuclear Chemistry, Bornova, Izmir 35100, Turkey

    Y. Yildirim

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  1. T. Ünak
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  2. U. Avcibaşi
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  3. Y. Yildirim
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Ünak, T., Avcibaşi, U. & Yildirim, Y. A radioanalytical technique for measurement of beta-glucuronidase activities. J Radioanal Nucl Chem 266, 503–506 (2005). https://doi.org/10.1007/s10967-005-0939-7

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  • DOI: https://doi.org/10.1007/s10967-005-0939-7

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