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Antemortem Stress Regulates Postmortem Glycolysis in Muscle by Deacetylation of Pyruvate Kinase M1 at K141

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Abstract

It is well known that preslaughter (antemortem) stress such as rough handling, transportation, a negative environment, physical discomfort, lack of consistent routine, and bad feed quality has a big impact on meat quality. The antemortem-induced poor meat quality is characterized by low pH, a pale and exudative appearance, and a soft texture. Previous studies indicate that antemortem stress plays a key role in regulating protein acetylation and glycolysis in postmortem (PM) muscle. However, the underlying molecular and biochemical mechanism is not clearly understood yet. In this study, we investigated the relationship between antemortem and protein acetylation and glycolysis using murine longissimus dorsi muscle isolated from ICR mice and murine muscle cell line C2C12 treated with epinephrine hydrochloride. Because adrenaline secretion increases in stressed animals, epinephrine hydrochloride was intraperitoneally injected epinephrine into mice to simulate pre-slaughter stress in this study to facilitate experimental operations and save experimental costs. Our findings demonstrated that protein acetylation in pyruvate kinase M1 (PKM1) form is significantly reduced by antemortem, and the reduced acetylation subsequently leads to an increase in PKM1 enzymatic activity which causes increased glycolysis in PM muscle. By using molecular approaches, we identified lysine 141 in PKM1 as a critical residue for acetylation. Our results in this study provide useful insight for controlling or improving meat quality in the future.

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Funding

This study was supported by the National Natural Science Foundation of China (Grant Number: 31571862; 5026301).

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Authors and Affiliations

  1. College of Animal Science, Xichang University, Xichang, 615013, Sichuan, China

    Shengwang Jiang & Qingwu W. Shen

  2. College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, Hunan, China

    Shengwang Jiang

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Contributions

Conceptualization, QWS and SJ; methodology, SJ; formal analysis, QWS, investigation, QWS; writing-original draft preparation, SJ; writing-review and editing, QWS; project administration, QWS; funding acquisition, QWS. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Qingwu W. Shen.

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Jiang, S., Shen, Q.W. Antemortem Stress Regulates Postmortem Glycolysis in Muscle by Deacetylation of Pyruvate Kinase M1 at K141. Protein J 43, 351–361 (2024). https://doi.org/10.1007/s10930-023-10178-6

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