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Identification of a Napin-Like Peptide from Eugenia malaccensis L. Seeds with Inhibitory Activity Toward Staphylococcus aureus and Salmonella Enteritidis

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Abstract

This study aimed to purify and characterize peptides from the seeds of Eugenia malaccensis, L. (jambo) with inhibitory activity against the foodborne pathogens Staphylococcus aureus and Salmonella Enteritidis. Crude extract (CE), precipitate fraction 30–60 % and molecules between 3.5 and 10 kDa obtained from precipitate fraction 30–60 % (Em2) showed inhibitory activity against the tested bacterial strains. The highest antibacterial activity was observed for Em2 against S. aureus. The major peak eluted at approximately 30 % in an acetonitrile gradient in reverse-phase chromatography of Em2 (Em2-F1 to Em2-F19), and it showed the highest antibacterial activity, which was twofold higher against S. aureus than against S. Enteritidis. MALDI-ToF spectra of Em2-F18 revealed a molecular mass of 1,231.1 Da and the amino acid sequence showed high identity to the napin family. These findings report for the first time a napin-like peptide from E. malaccensis L. seeds with potential to be applied as a new anti-Staphylococcus molecule.

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Abbreviations

AMPs:

Antimicrobial peptides

CE:

Crude extract

Fraction 30–60 %:

Fraction precipitate with amonium sulphate from jambo seeds

Em1 :

Molecules larger than 10 kDa from fraction 30–60 %

Em2 :

Molecules between 3.5 and 10 kDa from fraction 30–60 %

Em2-F1 to Em2-F19:

Cromatographic peaks obtained from Em2

Em2-F18:

Major cromatographic peak obtained from Em2 eluted at approximately 30 % acetonitrile

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Acknowledgments

The authors are grateful to the National Council for Scientific and Technological Development (CNPq, Brazil), CAPEs and FAPDF for financial support.

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Authors and Affiliations

  1. Laboratory of Microbial Processing in Foods, Department of Food Engineering, Center of Technology, Federal University of Paraíba, João Pessoa, Brazil

    Claudenise Caldas da Silva Dantas & Marciane Magnani

  2. Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paraíba, João Pessoa, Brazil

    Evandro Leite de Souza & Juscélio Donizete Cardoso

  3. Center of Biochemical and Proteomic Analysis, Catholic University of Brasília, Brasília, Brazil

    Loiane Alves de Lima, Kleber de Sousa Oliveira, Ludovico Migliolo, Simoni Campos Dias & Octávio Luiz Franco

Authors
  1. Claudenise Caldas da Silva Dantas
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  2. Evandro Leite de Souza
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  3. Juscélio Donizete Cardoso
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  4. Loiane Alves de Lima
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  5. Kleber de Sousa Oliveira
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  6. Ludovico Migliolo
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  7. Simoni Campos Dias
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  8. Octávio Luiz Franco
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  9. Marciane Magnani
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Correspondence to Marciane Magnani.

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da Silva Dantas, C.C., de Souza, E.L., Cardoso, J.D. et al. Identification of a Napin-Like Peptide from Eugenia malaccensis L. Seeds with Inhibitory Activity Toward Staphylococcus aureus and Salmonella Enteritidis. Protein J 33, 549–556 (2014). https://doi.org/10.1007/s10930-014-9587-5

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  • DOI: https://doi.org/10.1007/s10930-014-9587-5

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