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Green Synthesis of Silver Nanoparticles Using Exopolysaccharides Produced by Bacillus anthracis PFAB2 and Its Biocidal Property

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Abstract

Nowadays when control of environmental toxicity is a matter of concern, the focus of the researchers is to find an eco-friendly process. Considering the hazards associated with chemical synthesis of nanoparticles, green synthesis approaches have gained considerable attention for their sustainable nature in nanomedicine and nanobiotechnology. Here, exopolysaccharide (EPS) synthesized by a geothermal spring origin B. anthracis PFAB2 is used for green synthesis of silver nanoparticles (AgNPs). Elemental analysis of EPS-coated AgNPs exhibited solid peaks of silver (39.66%) along with oxygen and carbon. TEM analysis confirmed the hexagonal shape of the AgNPs. Polydispersity index (PDI) reinforced the moderately monodisperse nature of the nanoparticles. High negative zeta potential indicated longer shelf life, good colloidal nature and high dispersive nature of the AgNPs. The B. anthracis PFAB2 EPS-coated AgNPs demonstrated prospective biocidal characters for both gram positive and gram negative bacteria along with some potentially hazardous fungi compared with the conventional antimicrobials. The results can be much expedient in the future for treatment of microorganisms that are otherwise resistant to traditional antibiotics or antifungal drugs.

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Acknowledgements

Authors are thankful to UGC-Center of Advanced Study, Department of Botany, The University of Burdwan for pursuing research activities. AB is thankful for the financial assistance to SRF (State Funded) [Fc (Sc.) /RS/SF/BOT./2014-15/ 103 (3)]. AB also acknowledge the support of FONDECYT Iniciación No. 11190325 by Govt. Of Chile and Centro de Biotecnología de los Recursos Naturales (CenBio), Universidad Católica del Maule.

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Authors and Affiliations

  1. UGC‑Center of Advanced Study, Department of Botany, The University of Burdwan, Golapbag, Burdwan, West Bengal, 713104, India

    Aparna Banerjee & Rajib Bandopadhyay

  2. Centro de Investigación de Estudios Avanzados del Maule (CIEAM), Vicerrectoría de Investigación y Posgrado, Universidad Católica del Maule, Talca, Chile

    Aparna Banerjee

  3. Department of Chemistry, The University of Burdwan, Burdwan, West Bengal, 713104, India

    Debasis Das

  4. Biotechnology Engineering School, Universidad Católica del Maule, Talca, Chile

    Rodrigo Andler

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  1. Aparna Banerjee
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  2. Debasis Das
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  4. Rajib Bandopadhyay
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Correspondence to Rajib Bandopadhyay.

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Banerjee, A., Das, D., Andler, R. et al. Green Synthesis of Silver Nanoparticles Using Exopolysaccharides Produced by Bacillus anthracis PFAB2 and Its Biocidal Property. J Polym Environ 29, 2701–2709 (2021). https://doi.org/10.1007/s10924-021-02051-3

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