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Influence of Carbon Sources on The Production and Characterization of The Exopolysaccharide (EPS) by Bacillus sphaericus 7055 Strain

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Abstract

In this study, EPS produced by five Bacillus spp. strains was determined. The only one strain (B. sphaericus 7055) was selected due to its high EPS production and it was investigated by growing this strain in LB broth medium containing various carbon sources. The highest EPS production of this strain was found in medium containing fructose. However, the effect of different concentrations of fructose and molasses on EPS production by the strain was studied. The maximum EPS yield of the strain 7055 was recorded with 2.5% (w/v) fructose, also the highest EPS production was found in 2.5% (w/v) molasses. The strain 7055 was found to contain (98.6%) galactose and (1.4%) glucuronic acid in control medium whereas the composition of the strain 7055 (2.5% (w/v) fructose) was found to be (99.9%) neutral sugar and (0.1%) Glucuronic acid while the strain 7055 (2.5% (w/v) molasses) was found to contain (65.9%), neutral sugars and (34.1%) glucuronic acid.

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Acknowledgments

The authors would like to thank the members of Ankara University Faculty of Science, Department of Biology for providing the strains (B. sphaericus 1404 and B. sphaericus 7055).

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Authors and Affiliations

  1. Department of Biology, Faculty of Science and Arts, Nigde University, Campus, 51200, Nigde, Turkey

    Murat Yilmaz

  2. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Erciyes University, 38039, Kayseri, Turkey

    Gokcen Yuvali Celik & Dilsad Onbasili

  3. Molecular Biology Research Center, Gazi University, 06830, Ankara, Turkey

    Belma Aslim

Authors
  1. Murat Yilmaz
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  2. Gokcen Yuvali Celik
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  3. Belma Aslim
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  4. Dilsad Onbasili
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Correspondence to Dilsad Onbasili.

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Yilmaz, M., Celik, G.Y., Aslim, B. et al. Influence of Carbon Sources on The Production and Characterization of The Exopolysaccharide (EPS) by Bacillus sphaericus 7055 Strain. J Polym Environ 20, 152–156 (2012). https://doi.org/10.1007/s10924-011-0358-5

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  • DOI: https://doi.org/10.1007/s10924-011-0358-5

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