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Use of Bacteria as Fluorescent Penetrant for Penetrant Testing (PT)

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Abstract

Penetrant testing (PT) is a Nondestructive testing (NDT) method used for identifying and revealing surface cracks and defects in components. Using bacteria-based suspension as a fluorescent penetrant leads a new approach to PT with the advantages of using eco-friendly chemicals, fewer steps for test process and achieving low cost. The aim of the current study is to synthesize a new fluorescein isothiocyanate (FITC) conjugated Escherichia coli (E. coli) bacteria (FITC-E. coli) as fluorescent penetrant for detection of cracks on test materials. For this purpose, we tested performance of proposed bacteria suspension by means of its detection sensitivity using standardize test panels. Crack centers (6.35, 3.97 and 2.38 mm) and defects (0.2 × 0.4, 0.3 × 0.4 and 0.4 × 0.4 mm) were successfully detected via FITC-E. coli. Experimental results showed that the FITC-E. coli is efficient for detecting the cracks and defects. Research into development of bacteria-based fluorescent penetrant may bring new opportunities for NDT applications in the future.

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Acknowledgements

The authors thank to Machinery Department of the Ege Vocational School, Department of Biochemistry, Faculty of Science and also Aviation Higher Vocational School in Ege University.

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Authors and Affiliations

  1. Institute of Nuclear Sciences, Ege University, Bornova, 35100, Izmir, Turkey

    M. Karatay Kutman, F. Z. Biber Muftuler & O. Kozgus Guldu

  2. Ege Vocational School, Ege University, Bornova, 35100, Izmir, Turkey

    C Harmansah

Authors
  1. M. Karatay Kutman
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  2. F. Z. Biber Muftuler
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  3. C Harmansah
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  4. O. Kozgus Guldu
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Correspondence to F. Z. Biber Muftuler.

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Kutman, M.K., Muftuler, F.Z.B., Harmansah, C. et al. Use of Bacteria as Fluorescent Penetrant for Penetrant Testing (PT). J Nondestruct Eval 39, 15 (2020). https://doi.org/10.1007/s10921-020-0653-y

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  • DOI: https://doi.org/10.1007/s10921-020-0653-y

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