Abstract
In this study, Fe3O4 nanoparticles were first synthesized by co-precipitation method and then layered double hydroxides (LDHs) nanosheets were anchored onto Fe3O4 via an in-situ growth method to prepare LDHs@Fe3O4 carrier. The obtained magnets and LDHs@Fe3O4 carrier were characterized by XRD, FT-IR, N2 adsorption, HRSEM and HRTEM respectively. Using glutaraldehyde as a coupling agent, cellulase was immobilized onto this magnetic carrier by covalent binding. The effects of various parameters on enzyme activity of LDHs@Fe3O4 immobilized cellulase, including cellulase concentration, glutaraldehyde concentration, crosslinking time, pH, cellulase concentration, immobilization time and temperature were discussed in detailed. Moreover, thermal stability and operating stability of the immobilized cellulase were studied, the loaded amount of cellulase was measured, and the degradation performance of methoxychlor (MXC) by layered anchoring structure immobilized cellulase was evaluated.
Similar content being viewed by others
References
Q. Wang, D. O’Hare, Chem. Rev. 112, 4124–4155 (2012)
C. Mousty, V. Prévot, Anal. Bioanal. Chem. 405, 3513–3523 (2013)
S. Xu, M.C. Liao, H.Y. Zeng, C.R. Chen, H.Z. Duan, X.J. Liu et al., Appl. Clay Sci. 115, 124–131 (2015)
J. Zhang, H. Yan, T. Yang, Y. Zong, J. Environ. Manag. 92, 53 (2011)
K. Matsuura, Bulletin of the Chemical Society of Japan (The Chemical Society of Japan, Tokyo, 2017)
M. Komiyama, K. Yoshimoto, M. Sisido, K. Ariga, Bull. Chem. Soc. Jpn, 90, 967–1004 (2017)
X. Lian, Y. Fang, E. Joseph, Q. Wang, J. Li, S. Banerjee et al., Chem. Soc. Rev. 46, 3386 (2017)
G. Lawrence, P. Kalimuthu, M. Benzigar, K.J. Shelat, K.S. Lakhi, D.H. Park et al., Adv. Mater. (2017). https://doi.org/10.1002/adma.201702295
X.Z. Liu, Q. Zhang, Y.X. Liang, R.F. Zhang, Adv. Mater. Res. 1010–1012, 830–834 (2014)
X. Zheng, Q. Wang, Y. Jiang, J. Gao, Ind. Eng. Chem. Res. 51, 10140–10146 (2012)
D. Li, L. Luo, Z. Pang, L. Ding, Q. Wang, H. Ke et al., ACS Appl. Mater. Interfaces 6, 5144 (2014)
Y. Liu, Z. Zeng, G. Zeng, L. Tang, Y. Pang, Z. Li et al., Biores. Technol. 115, 21–26 (2012)
Y. Huang, Y. Xi, Y. Yang, C. Chen, H. Yuan, X. Liu, Chin. Sci. Bull. 59, 509–520 (2014)
M. Yuxiang Yang, P. Zhao, Y. Yao, Z. Huang, H. Dai, Yuan et al., J. Nanosci. Nanotechnol. 17, 1–8 (2017)
J. Yang, Y. Huang, Y. Yang, H. Yuan, X. Liu, J. Environ. Sci. 38, 52–62 (2015)
X. Liming, S. Xueliang, Biores. Technol. 91, 259–262 (2004)
J.A. Khan, S.K. Singh, Int. J. Plant. 1, 179–187 (2011)
L.I. Hui-Rong, J. Jiangsu Polytech. Univ. 69, 141–153 (2004)
M.M. Bradford, M. Bradford, Anal. Biochem. 72, 248–254 (1976)
L. Wu, X. Yuan, J. Sheng, J. Membr. Sci. 250, 167–173 (2005)
Y. Yang, Q. Wei, J. Zhang, Y. Xi, H. Yuan, C. Chen et al., Biochem. Eng. J. 97, 111–118 (2015)
Y. Zhong, Y. Liao, A. Gao, J. Hao, D. Shu, Y. Huang et al., J. Alloys Compd. 669, 146–155 (2016)
S. Yin, J. Li, H. Zhang, Green Chem. (2016). https://doi.org/10.1039/c5gc00709g
R. Fernández-Lafuente, V. RodríGuez, C. Mateo, G. Fernández-Lorente, P. Arminsen, P. Sabuquillo et al., J. Mol. Catal. B 7, 173–179 (1999)
K. Kang, C. Kan, A. Yeung, D. Liu, Mater. Sci. Eng. C 26, 664–669 (2006)
X.N. Dai, W.G. Hou, H.D. Duan, P. Ni. Colloid Surf A 295, 139–145 (2007)
N. Srivastava, M. Srivastava, P.K. Mishra, P.W. Ramteke, Front. Microbiol. 7, 514 (2016)
O. Barbosa, R. Torres, C. Ortiz, Á BerenguerMurcia, R.C. Rodrigues, R. Fernandezlafuente, Biomacromology 14, 2433 (2013)
Acknowledgements
This work was supported by the National Natural Science Foundation of China (20577010, 20971043); and the Fundamental Research Funds for the Central Universities.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Pei, J., Huang, Y., Yang, Y. et al. A Novel Layered Anchoring Structure Immobilized Cellulase via Covalent Binding of Cellulase on MNPs Anchored by LDHs. J Inorg Organomet Polym 28, 1624–1635 (2018). https://doi.org/10.1007/s10904-018-0838-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10904-018-0838-3