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Fluorescent Turn-On Sensing of Zinc(II) and Alkaline Phosphatase Activity Using a Pyridoxal-5’-Phosphate Derived Schiff Base

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Abstract

A novel Zn2+ ion and alkaline phosphatase (ALP) selective fluorescence turn-on sensor L was developed by reacting pyridoxal 5’-phosphate (PLP) with hydrazine. Sensor L shows significant flurescence enhancement at 476 nm due to the formation of a L-Zn2+ complex in 1:1 binding stoichiometry with the association constant of 3.1⋅104 M− 1. Using L, the concentration of Zn2+ can be detected down to 2.34 µM, and the practical utility of L was validated by quantifying Zn2+ in real water samples. Additionally, the receptor L was applied to mimic the dephosphorylation reaction catalysed by the enzyme ALP and the resulted fluorescence change was monitored to detect the ALP activity.

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All data generated during this study are included in this published article.

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Author notes

  1. Amit Kumar and Yachana Upadhyay contributed equally to this work.

Authors and Affiliations

  1. Department of Chemistry, SV National Institute Technology, Surat, 395007, Gujarat, India

    Amit Kumar & Suban K. Sahoo

  2. Department of Chemistry, Malaviya National Institute of Technology, Jaipur, 302017, Rajasthan, India

    Yachana Upadhyay

  3. Department of Chemistry, ACC Wing, IMA Dehradun, Uttarakhand, 248007, India

    Rati Kanta Bera

Authors
  1. Amit Kumar
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  2. Yachana Upadhyay
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  3. Rati Kanta Bera
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  4. Suban K. Sahoo
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Contributions

All authors contributed to the study. The Investigation, Validation, Formal analysis, Data curation, Writing-original draft were performed by Amit Kumar and Yachana Upadhyay. Rati Kanta Bera helps in Formal analysis. The Conceptualization, Resources, Supervision, and Writing-review & editing were performed by Suban K Sahoo.

Corresponding author

Correspondence to Suban K. Sahoo.

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Kumar, A., Upadhyay, Y., Bera, R.K. et al. Fluorescent Turn-On Sensing of Zinc(II) and Alkaline Phosphatase Activity Using a Pyridoxal-5’-Phosphate Derived Schiff Base. J Fluoresc 33, 2469–2478 (2023). https://doi.org/10.1007/s10895-023-03254-8

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