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A Rapid Near-Infrared Fluorescent Probe for Cysteine Based on Isophorone and its Application in B16 Cell Imaging

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Abstract

A novel near-infrared fluorescent probe SWJT-5 based on dicyanoisophorone was synthesized. It achieved the rapid (within 40 s) and discriminative detection of Cys over Hcy and GSH with a large Stokes shift (205 nm). It showed high selectivity and sensitivity for Cys, and had an obvious enhancement of fluorescence emission. The detection limit was 0.43 μM. This probe also had low background interference and little damage to biological samples. Therefore, SWJT-5 had been applied to bioimaging in living cells successfully.

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Acknowledgements

We would like to thank Analytical and Testing Center of Southwest Jiaotong University for the laser confocal microscope test.

Funding

This work was supported by the National Natural Science Foundation of China (No. 22071200), and the Fundamental Research Funds for the Central Universities (Nos. 2682020CX55, 2682021ZTPY011 and XJ2021KJZK004) by MoE of China. We also thank Science and Technology Department of Sichuan Province (2020JDRC0021), and Key Research Project of Sichuan Province Science and Technology Plan (2020YFS0489).

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  1. Yan-Ya Wang and Xue-Shuang Yu contributed equally to this work.

Authors and Affiliations

  1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People’s Republic of China

    Yan-Ya Wang, Xue-Shuang Yu, Xin-Jie Li, Hong-Bo Liu, Ya-Wen Wang & Yu Peng

  2. Department of Neurology, The Third People’s Hospital of Chengdu, the Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610031, People’s Republic of China

    Xi Zhu

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  1. Yan-Ya Wang
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  2. Xue-Shuang Yu
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  5. Xi Zhu
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  6. Ya-Wen Wang
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  7. Yu Peng
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Contributions

Yan-Ya Wang: conceived the work and performed most of the experiments. Xue-Shuang Yu, Xin-Jie Li, and Hong-Bo Liu carried out the bioimaging. Yan-Ya Wang, Xue-Shuang Yu, Xin-Jie Li, Hong-Bo Liu, Xi Zhu,Ya-Wen Wang, and Yu Peng analyzed the data and wrote the manuscript. Yu Peng supervised the project and conceived the style of the manuscript.

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Correspondence to Yu Peng.

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Wang, YY., Yu, XS., Li, XJ. et al. A Rapid Near-Infrared Fluorescent Probe for Cysteine Based on Isophorone and its Application in B16 Cell Imaging. J Fluoresc 32, 1661–1667 (2022). https://doi.org/10.1007/s10895-022-02964-9

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  • DOI: https://doi.org/10.1007/s10895-022-02964-9

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