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Enhanced Hydrodynamic Radius of AOT/n-heptane/Water Reverse Micellar System Through Altered Electrostatic Interactions and Molecular Self-Assemblies

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Abstract

We have demonstrated a unique approach to alter the aqueous pool size of an AOT/n-heptane/water reverse micellar system. A positively charged dye Rhodamine B (RhB) and negatively charged Rose Bengal (RB) were incorporated in the reverse micellar pool to investigate the effect of electrostatic interactions and stacking effects among the dye molecules on the AOT/n-heptane/water interface. Dynamic light scattering revealed increase in reverse micellar pool size in presence of positively charged dye aggregates at the oil–water interface. However, less expansion was observed in presence of negatively charged dye aggregates (RB). This confirms the role of electrostatic interaction in modulating the hydrodynamic radius. A head-to-tail type of stacking of RhB molecules at the interface favors this expansion. The differences in stacking of the two dyes inside the reverse micelles and their torsional mobility indicated the role of the reverse micellar interface and H-bonding ability of the microenvironment on dye aggregation. Conductivity measurements demonstrated a significant drop in percolation temperature of the reverse micellar system in presence of dye aggregates. This confirms the effect of dye aggregation and electrostatic interaction on such expansion. This strategy can be exploited for solubilizing greater amounts and a wider variety of drug molecules in microemulsions.

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Acknowledgements

S De acknowledges the support received from DST-PURSE-II grant of the University of Kalyani. S. Das acknowledges the CSIR Senior Research Associateship (Scientist’s Pool Scheme), [Grant No. 8685-A/ 2014 Pool]. B. Mandal thanks DST-INSPIRE for financial support. The fluorescence life time studies were performed at IACS Kolkata and Jadavpur University, Kolkata. Prof. Tarakdas Basu of the Department of Biochemistry and Biophysics, University of Kalyani is acknowledged for letting us use the DLS facility in his laboratory. University of Kalyani is acknowledged for providing the basic infrastructure for research.

Funding

S De acknowledges the support received from DST-PURSE-II grant of the University of Kalyani. S. Das acknowledges the CSIR Senior Research Associateship (Scientist’s Pool Scheme), [Grant No. 8685-A/ 2014 Pool]. B. Mandal thanks DST-INSPIRE for financial support.

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Susmita Das contributed to the experimental work, writing the manuscript and analysis of data. Ranju Prasad Mandal contributed to the experimental work and part of data analysis. Barun Mandal contributed to the experimental work. Swati De contributed to writing the manuscript and overall supervision and planning of the research work which was carried out in her research laboratory.

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Correspondence to Swati De.

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Das, S., Mandal, R.P., Mandal, B. et al. Enhanced Hydrodynamic Radius of AOT/n-heptane/Water Reverse Micellar System Through Altered Electrostatic Interactions and Molecular Self-Assemblies. J Fluoresc 31, 1475–1488 (2021). https://doi.org/10.1007/s10895-021-02760-x

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