Skip to main content
SpringerLink
Log in

Cation Effect on Slow Release from Alginate Beads: A Fluorescence Study

  • ORIGINAL PAPER
  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

In this study, spherical alginate beads containing pyranine (P y ) as a fluorescence probe were prepared by ionotropic gelation of a sodium alginate solution. The steady state fluorescence technique was used to study pyranine release from the alginate beads crosslinked with calcium, barium and aluminum ions, respectively. The slow release of P y was observed with the time drive mode of the spectrophotometer at 512 nm. Fluorescence emission intensity (I p ) from P y was monitored during the release process, and the encapsulation efficiency (EE) of pyranine from the alginate beads was calculated. The Fickian Diffusion model was used to measure the release coefficients, D sl . It was seen that the slow release coefficients of pyranine from the alginate beads crosslinked with Ca2+, Ba2+, and Al3+ ions increased in the following order: D sl (Al3+)> D sl (Ca2+)> D sl (Ba2+). In contrast, the initial amount of pyranine and EE into the beads showed the reverse behavior.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Papageorgiou SK, Kouvelos EP, Favvas EP, Sapalidis AA, Romanos GE, Katsaros FK (2010) Metal–carboxylate interactions in metal–alginate complexes studied with FTIR spectroscopy. Carbohydr Res 345:469–473

    Article  CAS  PubMed  Google Scholar 

  2. Grant GT, Morris ER, Rees DA, Smith PJC, Thom D (1973) Biological interactions between polysaccharides and divalent cations: the egg-box model. FEBS Lett 32:195–198

    Article  CAS  Google Scholar 

  3. Kikuchi A, Kawabuchi M, Sugihara M, Sakurai Y, Okano T (1997) Pulsed dextran release from calcium-alginate gel beads. J Control Release 47:21–29

    Article  CAS  Google Scholar 

  4. Işıklan N, İnal M, Kurşun F, Ercan G (2011) pH responsive itaconic acid grafted alginate microspheres for the controlled release of nifedipine. Carbohydr Polym 84:933–943

    Article  Google Scholar 

  5. Bierhalz AKC, da Silva MA, Kieckbusch TG (2012) Natamycin release from alginate/pectin films for food packaging applications. J Food Eng 110:18–25

    Article  CAS  Google Scholar 

  6. Banerjee A, Nayak D, Lahiri S (2007) Speciation-dependent studies on removal of arsenic by iron-doped calcium alginate beads. Appl Radiat Isot 65:769–775

    Article  CAS  PubMed  Google Scholar 

  7. Tezcan F, Günister E, Özen G, Erim FB (2012) Biocomposite films based on alginate and organically modified clay. Int J Biol Macromol 50:1165–1168

    Article  CAS  PubMed  Google Scholar 

  8. Darrabie MD, Kendall WF, Opara EC (2006) Effect of alginate composition and gelling cation on microbead swelling. J Microencapsul 23:613–621

    Article  CAS  PubMed  Google Scholar 

  9. Mørch YA, Donati I, Strand BL, Skjåk-Braek G (2006) Effect of Ca2+, Ba2+ and Sr2+ on alginate microbeads. Biomacromolecules 7:1471–1480

    Article  PubMed  Google Scholar 

  10. Anal AK, Stevens WF (2005) Chitosan–alginate multilayer beads for controlled release of ampicillin. Int J Pharm 290:45–54

    Article  CAS  PubMed  Google Scholar 

  11. Cook MT, Tzortzis G, Charalampopoulos D, Khutoryanskiy VV (2012) Microencapsulation of probiotics for gastrointestinal delivery. J Control Release 162:56–67

    Article  CAS  PubMed  Google Scholar 

  12. Chretien C, Chaumeil JC (2005) Release of a macromolecular drug from alginate-impregnated microspheres. Int J Pharm 304:18–28

    Article  CAS  PubMed  Google Scholar 

  13. Nochos A, Douroumis D, Bouropoulos N (2008) In vitro release of bovine serum albumin from alginate/HPMC hydrogel beads. Carbohydr Polym 74:451–457

    Article  CAS  Google Scholar 

  14. Torre ML, Maggi L, Vigo D, Galli A, Bornaghi V, Maffeo G, Conte U (2000) Controlled release of swine semen encapsulated in calcium alginate beads. Biomaterials 21:1493–1498

    Article  CAS  PubMed  Google Scholar 

  15. Abd El-Ghaffar MA, Hashem MS, El-Awady MK, Rabie AM (2012) pH-sensitive sodium alginate hydrogels for riboflavin controlled release. Carbohydr Polym 89:667–675

    Article  CAS  Google Scholar 

  16. Tanaka H, Irie S (1988) Preparation of stable alginate beads in electrolyte solutions using Ba2+ and Sr2+. Biotechnol Tech 2:115–120

    Article  CAS  Google Scholar 

  17. Şanlı O, Işıklan N (2006) Controlled release formulations of carbaryl based on copper alginate, barium alginate, and alginic acid beads. J Appl Polym Sci 102:4245–4253

    Article  Google Scholar 

  18. Torre ML, Faustini M, Norberti R, Stacchezzini S, Maggi L, Maffeo G, Conte U, Vigo D (2002) Boar semen controlled delivery system: storage and in vitro spermatozoa release. J Control Release 85:83–89

    Article  CAS  PubMed  Google Scholar 

  19. Nokhodchi A, Tailor A (2004) In situ cross-linking of sodium alginate with calcium and aluminum ions to sustain the release of theophylline from polymeric matrices. Il Farmaco 59:999–1004

    Article  CAS  PubMed  Google Scholar 

  20. Gan BS, Krump E, Shrode LD, Grinstein S (1998) Loading pyranine via purinergic receptors or hypotonic stress for measurement of cytosolic pH by imaging. Am J Physiol Cell Physiol 275:C1158–C1166

    CAS  Google Scholar 

  21. Mondal SK, Sahu K, Sen P, Roy D, Ghosh S, Bhattacharyya K (2005) Excited state proton transfer of pyranine in a γ-cyclodextrin cavity. Chem Phys Lett 412:228–234

    Article  CAS  Google Scholar 

  22. Evingür GA, Tezcan F, Erim FB, Pekcan Ö (2012) Monitoring the gelation of polyacrylamide-sodium alginate composite by fluorescence technique. Phase Transit 85:530–541

    Article  Google Scholar 

  23. Pekcan Ö, Tari Ö (2004) A fluorescence study on the gel-to-sol transition of κ-carrageenan. Int J Biol Macromol 34:223–231

    Article  CAS  PubMed  Google Scholar 

  24. Pekcan Ö, Kara S (2005) Cation effect on thermal transition of iota-carrageenan: a photon transmission study. J Biomater Sci Polym Ed 16:317–333

    Article  CAS  PubMed  Google Scholar 

  25. Kara S, Arda E, Pekcan Ö (2007) Monovalent and divalent cation effects on phase transitions of ı-carrageenan. J Bioact Compat Polym 22:42–61

    Article  CAS  Google Scholar 

  26. Evingür GA, Karslı K, Pekcan Ö (2006) Monitoring small molecule diffusion into hydrogels at various temperatures by fluorescence technique. Int J Pharm 326:7–12

    Article  PubMed  Google Scholar 

  27. Ataman E, Pekcan Ö (2007) Small molecule diffusion into swelling Iota-Carrageenan gels: a fluorescence study. J Biomol Struct Dyn 24:505–513

    Article  CAS  PubMed  Google Scholar 

  28. Evingür GA, Pekcan Ö (2007) Sorption and slow release kinetics of PAAm gels at various temperatures. J Polym Eng 27:583–595

    Article  Google Scholar 

  29. Kara S, Gacal B, Tunç D, Yağcı T, Pekcan Ö (2012) Sorption and desorption of PVA-Pyrene Chains in and out of Agarose gel. J Fluoresc 22:1073–1080

    Article  CAS  PubMed  Google Scholar 

  30. Crank J (1975) The mathematics of diffusion. Clarendon, Oxford

    Google Scholar 

  31. Crank J, Park GS (1968) Diffusion in polymer. Academic, London

    Google Scholar 

  32. Bajpai SK, Sharma S (2004) Investigation of swelling/degradation behavior of alginate beads crosslinked with Ca2+ and Ba2+ ions. React Funct Polym 59:129–140

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Experiments were carried out at the Spectroscopy Laboratory of the Department of Molecular Biology and at the Capillary Electrophoresis Laboratory of the Department of Chemistry at Istanbul Technical University.

Author information

Authors and Affiliations

  1. Faculty of Science and Letters, Istanbul Technical University, 34469, Maslak, Istanbul, Turkey

    Hakan Kaygusuz & F. Bedia Erim

  2. Faculty of Science and Letters, Kadir Has University, 34083, Cibali, Istanbul, Turkey

    Önder Pekcan

  3. Faculty of Science and Letters, Piri Reis University, 34940, Tuzla, Istanbul, Turkey

    Gülşen Akın Evingür

Authors
  1. Hakan Kaygusuz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Bedia Erim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Önder Pekcan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gülşen Akın Evingür
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gülşen Akın Evingür.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaygusuz, H., Erim, F.B., Pekcan, Ö. et al. Cation Effect on Slow Release from Alginate Beads: A Fluorescence Study. J Fluoresc 24, 161–167 (2014). https://doi.org/10.1007/s10895-013-1282-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10895-013-1282-y

Keywords

Search

Navigation