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A Highly Sensitive Fluorescent Probe for HClO and Its Application in Live Cell Imaging

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Abstract

A new rhodamine-based probe 1 was designed and synthesized as a new fluorescent molecular probe for HClO in PBS buffer at physiological condition. The free probe 1 almost nonfluorescence, however, a drastic enhancement of fluorescence intensity was observed in the presence of HClO. The new probe 1 exhibits good sensitivity and selectivity for HClO over other reactive oxygen and/or nitrogen species in PBS buffer, and the probe was successfully applied to image endogeneous HClO in the living cells.

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Acknowledgments

This work was supported by the Science Research Foundation of Central South University.

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Authors and Affiliations

  1. Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People’s Republic of China

    Qing-Ping Zuo, Zuo-Jun Li, Bin Li, Chun-Jiang Wang, Shi-Kun Liu & Hai-Qiang Liao

  2. Department of Pharmacy, The first Hospital of Changsha City, Changsha, Hunan, China

    Qing-Ping Zuo & Yong-Hua Hu

  3. College of Pharmacy, Central South University, Changsha, Hunan, China

    Zuo-Jun Li, Chun-Jiang Wang & Shi-Kun Liu

  4. Center for Experimental Medicine, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People’s Republic of China

    Li-Hua Huang

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  1. Qing-Ping Zuo
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  2. Zuo-Jun Li
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  3. Yong-Hua Hu
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  4. Bin Li
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  8. Hai-Qiang Liao
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Correspondence to Zuo-Jun Li or Shi-Kun Liu.

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Zuo, QP., Li, ZJ., Hu, YH. et al. A Highly Sensitive Fluorescent Probe for HClO and Its Application in Live Cell Imaging. J Fluoresc 22, 1201–1207 (2012). https://doi.org/10.1007/s10895-012-1067-8

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  • DOI: https://doi.org/10.1007/s10895-012-1067-8

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