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Spectroscopic Investigation on the Synergistic Effects of Ultrasound and Dioxopromethazine Hydrochloride on Protein

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Abstract

The bovine serum albumin (BSA) was selected as a target molecule, the sonodynamic damage to protein in the presence of dioxopromethazine hydrochloride (DPZ) and its mechanism were studied by means of absorption and fluorescence spectra. The results of hyperchromic effect of absorption spectra and quenching of intrinsic fluorescence spectra indicated that the synergistic effects of ultrasound and DPZ could induce the damage of BSA molecules. The damage degree of BSA molecules increased with the increase of ultrasonic irradiation time and DPZ concentration. The results of synchronous fluorescence and three-dimensional fluorescence spectra further confirmed that the synergistic effects of ultrasound and DPZ induced the damage of BSA molecules. The results of oxidation-extraction photometry with several reactive oxygen species (ROS) scavengers indicated that the damage of BSA molecules could be mainly due to the generation of ROS, in which both 1O2 and ·OH were the important mediators of the ultrasound-inducing BSA molecules damage in the presence of DPZ.

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Authors and Affiliations

  1. Department of Chemistry, Northeastern University, Shenyang, 110819, People’s Republic of China

    Ling-Ling He, Bin Liu & Shu-Kun Xu

  2. College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, People’s Republic of China

    Ling-Ling He & Ya-Guang Sun

  3. School of Pharmaceutical Sciences, Liaoning University, Shenyang, 110036, People’s Republic of China

    Xin Wang & Bin Liu

  4. Department of Chemistry, Liaoning University, Shenyang, 110036, People’s Republic of China

    Jun Wang

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  1. Ling-Ling He
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  2. Xin Wang
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Correspondence to Shu-Kun Xu.

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He, LL., Wang, X., Liu, B. et al. Spectroscopic Investigation on the Synergistic Effects of Ultrasound and Dioxopromethazine Hydrochloride on Protein. J Fluoresc 21, 1847–1856 (2011). https://doi.org/10.1007/s10895-011-0879-2

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  • DOI: https://doi.org/10.1007/s10895-011-0879-2

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