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Spectroscopic Study of the Interaction of Styrylcyanine Dyes Sbo, Sil and Their Derivatives with Bovine Serum Albumin

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Abstract

The spectral-luminescent characteristics of newly synthesized styrylcyanine dyes on the base of dyes Sbo ((E)-2-(4-(dimethylamino)styryl)-3-methylbenzo[d]oxazol-3-ium iodide) and Sil ((E)-2-(4-(dimethylamino)styryl)-1,3,3-trimethyl-3H-indolium perchlorate) in aqueous solutions without and in the presence of bovine serum albumin (BSA) were studied. It was established that the absorption spectra of dyes Tol-6, Dbo-10 and Dil-10 with increasing amount of BSA appear new bands with λmax = 505 nm, λmax = 512 nm and λmax = 566 nm, respectively, whose intensity increases in proportion to the amount of albumin. The intensity of the glow of the main band of fluorescence in the presence of BSA sharply increases. The binding constant (K) and the number of binding sites (N) of studied dyes with BSA were determined. The dependence of binding constants with BSA on the dipole moment of dye molecules was determined, which indicates that besides electrostatic forces of attraction between molecules styrylcyanine dyes with BSA, hydrophobic interactions are essential.

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Acknowledgments

The author is grateful to Dr. S.M. Yarmoluk from the Institute of Molecular Biology and Genetics NAS of Ukraine for allotment of dyes and Professor N. Nizomov from Samarkand State University for his fruitful participation in the discussion of the results. This work was supported by grant 3104 (STCU, Ukraine).

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  1. Physics department, Samarkand State University, University blvd. 15, 140104, Samarkand, Uzbekistan

    Eldar N. Kurtaliev

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  1. Eldar N. Kurtaliev
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Correspondence to Eldar N. Kurtaliev.

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Kurtaliev, E.N. Spectroscopic Study of the Interaction of Styrylcyanine Dyes Sbo, Sil and Their Derivatives with Bovine Serum Albumin. J Fluoresc 21, 1713–1719 (2011). https://doi.org/10.1007/s10895-011-0866-7

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  • DOI: https://doi.org/10.1007/s10895-011-0866-7

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