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Fluorescent Probing of Protein Bovine Serum Albumin Stability and Denaturation Using Polarity Sensitive Spectral Response of a Charge Transfer Probe

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Abstract

The polarity sensitive photo-induced intra-molecular charge transfer (ICT) fluorescence probe (E)-3-(4-methylamino-phenyl)-acrylic acid ethyl ester (MAPAEE) has been used to study the model protein Bovine Serum Albumin (BSA) in its native and thermal and urea induced denatured states. The interaction between BSA and the regular surfactant Sodium Dodecyl Sulphate (SDS) as well as the biologically relevant steroid-based amphiphile Sodium Deoxycholate (NaDC) has also been very keenly followed using this ICT probe. The variation of micellar properties of both SDS and NaDC with increasing ionic strengths and in presence of the chaotrope urea has also been well documemted by the same probe. Steady-state spectroscopy, FRET, and fluorescence anisotropy measurements have been used to gain better insight into these processes and the molecule MAPAEE to be a full-bodied fluorescent probe for studying such intricate biological systems, their properties and interactions.

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Acknowledgement

This work is supported by CSIR, India (Project no. 01(2161)07/EMR-II) and DST, India (Project no. SR/S1/PC/26/2008). The authors would like to thank Prof. T. Ganguly of IACS, Kolkata for allowing them the fluorescence lifetime measurements

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Authors and Affiliations

  1. Department of Chemistry, University of Calcutta, 92, A. P. C. Road, Kolkata, 700009, India

    Shalini Ghosh, Sankar Jana & Nikhil Guchhait

  2. Department of Physical Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, 700032, India

    Debnarayan Nath

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  1. Shalini Ghosh
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  2. Sankar Jana
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  3. Debnarayan Nath
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  4. Nikhil Guchhait
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Correspondence to Nikhil Guchhait.

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Ghosh, S., Jana, S., Nath, D. et al. Fluorescent Probing of Protein Bovine Serum Albumin Stability and Denaturation Using Polarity Sensitive Spectral Response of a Charge Transfer Probe. J Fluoresc 21, 365–374 (2011). https://doi.org/10.1007/s10895-010-0725-y

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  • DOI: https://doi.org/10.1007/s10895-010-0725-y

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