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Development of Fluorescent Array Based on Sol-Gel/Chitosan Encapsulated Acetylcholinesterase and pH Sensitive Oxazol-5-one Derivative

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Abstract

A highly sensitive fluorescent enzyme array for quantitative acetylcholine detection is developed. The enzyme array has been constructed by spotting of pH sensitive fluorophore 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacycloopentadecyl)benzylidene]oxazol-5-one and acetylcholinesterase doped in tetraethoxysilane / chitosan matrix via a microarrayer. The constructed tetraethoxysilane / chitosan network provided a microenvironment in which the enzyme molecule was active biologically. The optimal operational conditions for the array developed were investigated. The response of the developed biosensor array to acetylcholine was highly reproducible (RSD = 3.27%, n = 6). A good linearity was observed for acetylcholine in the concentrations up to 1 × 10−8 M, with a detection limit of 0.27 × 10−8 M.

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Authors and Affiliations

  1. Faculty of Arts and Sciences, Department of Chemistry, University of Dokuz Eylul, Tinaztepe, 35160, Izmir, Turkey

    Gulsiye Ozturk, Serap Alp & Yavuz Ergun

  2. Department of Medical Engineering and Biotechnology, University of Applied Sciences Jena, Carl-Zeiss-Promenade 2, 07745, Jena, Germany

    Gulsiye Ozturk, Karl-Heinz Feller & Kay Dornbusch

  3. Faculty of Science, Department of Biochemistry, Ege University, Bornova, 35100, Izmir, Turkey

    Suna Timur

Authors
  1. Gulsiye Ozturk
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  2. Karl-Heinz Feller
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  3. Kay Dornbusch
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  4. Suna Timur
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  5. Serap Alp
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  6. Yavuz Ergun
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Correspondence to Gulsiye Ozturk.

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Ozturk, G., Feller, KH., Dornbusch, K. et al. Development of Fluorescent Array Based on Sol-Gel/Chitosan Encapsulated Acetylcholinesterase and pH Sensitive Oxazol-5-one Derivative. J Fluoresc 21, 161–167 (2011). https://doi.org/10.1007/s10895-010-0700-7

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  • DOI: https://doi.org/10.1007/s10895-010-0700-7

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