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Resonance Scattering Spectral Detection of Catalase Activity Using Au@Ag Nanoparticle as Probe and Coupling Catalase Catalytic Reaction with Fenton Reaction

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Abstract

The AucoreAgshell (Au@Ag) nanoparticles in size of 30 nm were prepared using 10 nm gold nanoparticles as seeds at 90°C, and were purified by high-speed centrifugation to remove the excess trisodium citrate to obtain Au@Ag nanoprobe. In the medium of pH 4.0 acetate buffer solution—7.2 μmol/L H2O2–67 μmol/L Fe(II), Au@Ag nanoparticles exhibited a resonance scattering (RS) peak at 538 nm. Upon addition of Catalase (Ct), the system produced hydroxyl radical that oxidized the Au@Ag nanoprobe to form the AuAg nanoparticles with partly bare nanogold. Those AuAg nanoparticles aggregated to large nanoclusters that led to the RS peak wavelength red-shift and its RS peak intensity enhanced. The catalase activity (C) is linear to the enhanced RS intensity (ΔI) in the range of 6 to 2,800 U/L, with regression equation of ΔI = 0.168 C-0.2, the correlation coefficient of 0.9952, and detection limit of 2.8 U/L. This method was applied to the detection of serum samples, and the results were agreement with that of the spectrophotometry. A new catalytic mechanism of catalase was proposed with oxywater principle that was agreement with the results of resonance scattering spectroscopy, absorption spectrophotometry, transmission electron microscopy and laser scattering.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 20667001, 20865002), Natural Science Foundation of Guangxi (No.0832260) and the Research Funds of Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment (Nos. 0701Z022, 0701k008)

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Authors and Affiliations

  1. Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, 541004, P R China

    Aihui Liang & Zhiliang Jiang

  2. Department of Material and Chemical Engineering, Key Laboratory of New Processing Technology for Nonferrous Metals and Materials of the Education Ministry, Guilin University of Technology, Guilin, 541004, P R China

    Yueyuan Liang & Zhiliang Jiang

  3. Animal Science and Technology Collage, Guangxi University, Nanning, 531004, P R China

    Hesheng Jiang

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  1. Aihui Liang
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  2. Yueyuan Liang
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Correspondence to Aihui Liang or Zhiliang Jiang.

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Liang, A., Liang, Y., Jiang, Z. et al. Resonance Scattering Spectral Detection of Catalase Activity Using Au@Ag Nanoparticle as Probe and Coupling Catalase Catalytic Reaction with Fenton Reaction. J Fluoresc 19, 1009–1015 (2009). https://doi.org/10.1007/s10895-009-0500-0

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  • DOI: https://doi.org/10.1007/s10895-009-0500-0

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