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Specificity of Cyanine Dye L-21 Aggregation in Solutions with Nucleic Acids

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Abstract

Optical spectroscopy experiments were used to study the features of cyanine dye 3,3′-dimethyl-9-(2-thienyl)-thiacarbocyanine iodide (L-21) aggregation in binary solutions DMF:Tris–HCl buffer (pH = 8) containing nucleic acids (DNA or RNA). The appearance of absorption and luminescence bands associated with J-aggregates and dimers that are formed within the minor groove of DNA has been observed. The model of L-21 J-aggregate structure is proposed. It has been found that dimers are the building blocks of L-21 J-aggregates. Disorientation in dimers caused by the minor groove curvature is reason of observation of Davydov splitting in absorption spectrum of L-21 J-aggregates. In the solution containing DNA the absorption and luminescence bands of L-21 J-aggregates exhibit the specific properties that allows the dye L-21 to be used as a fluorescent probe for DNA detection.

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Acknowledgment

We gratefully acknowledge Dr. S. S. Lukashov (Institute of Molecular Biology and Genetics of NAS of Ukraine) for providing dye L-21.

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Authors and Affiliations

  1. Institute for Scintillation Materials, STC “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001, Kharkov, Ukraine

    G. Ya. Guralchuk, A. V. Sorokin, I. K. Katrunov, S. L. Yefimova, A. N. Lebedenko & Yu. V. Malyukin

  2. Institute for Molecular Biology and Genetics, NAS of Ukraine, 150 Zabolotnogo str., 03143, Kyiv, Ukraine

    S. M. Yarmoluk

Authors
  1. G. Ya. Guralchuk
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  2. A. V. Sorokin
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  3. I. K. Katrunov
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  4. S. L. Yefimova
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  5. A. N. Lebedenko
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  6. Yu. V. Malyukin
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  7. S. M. Yarmoluk
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Correspondence to A. V. Sorokin.

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Guralchuk, G.Y., Sorokin, A.V., Katrunov, I.K. et al. Specificity of Cyanine Dye L-21 Aggregation in Solutions with Nucleic Acids. J Fluoresc 17, 370–376 (2007). https://doi.org/10.1007/s10895-007-0201-5

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  • DOI: https://doi.org/10.1007/s10895-007-0201-5

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