Abstract
A fluorescent probe, ABM, aminoderivative of benzanthrone, synthesized in the Department of Organic Chemistry of the Riga Technical University (Latvia), has been successfully used to characterize changes in the structural and functional properties of cell membranes during different pathologies. In the present study the physicochemical properties and the functional activity of the peripheral blood mononuclear cells (lymphocytes—Ly) in patients with rheumatoid arthritis (RA) were studied using the ABM probe. Intensity of the ABM fluorescence in the celi suspension, functional activity of the ly anisotropy of the membranes differ patients with different titres of rheumatoid factor in blood. Patients with seropositive RA had decreased proliferative activity and lower number of iy in blood plasma indicating greater alterations of the immunoregulating processes in these patients as compared to patients with seronegative RA. In the latter the Ly deficiency is compensated to some extent by increased proliferation activity of these cells. The ABM fluorescence intensity correlated not only with membrane anisotropy (r = 0.97, but also with the proliferation activity of the Ly (r = 0.98). The above parameters correlated with the clinical manifestations of the disease. The results indicate that the fluorescent probe ABM is useful for screening the physicochemical status of Ly membranes and the proliferation activity of these cells in RA patients.
Similar content being viewed by others
References
G. V. Bail and W. J. Koopmen (1986). Clinical Rheumatology, Saunders, Philadelphia, 367 pp.
A. Skapenko, J. Wendler, P. E. Lipsky, J. R. Kalden, and H. Schulzekoops (1999). Altered memory T-cell differentiation in patients with early rheumatoid arthritis. J. Immunol. 49(4), 298–304.
J. R. Lakowicz (1984). Principles of Fluorescence Spectroscopy, Plenum, New York.
I. Kalnina and I. Meirovics (1999). A new fluorescent probe, ABM; properties and application in clinical diagnostics. J. Fluoresc. 9. 27–32.
D. P. Stitas (1999). Basic and Clinical Immunology, 7th ed., Appleton and Lange, pp. 263–283.
D. B. Duncan (1970). Qucry multiple comparison methods for comparig regression coefficient. Biometrics 26, 141–143.
A. Anel, K. Noval, B. Gonsalez, J. M. Torres, Z. Mishal, J. Uriel, and A. Pineiro (1990). Fatty acid metabolism in human lymphocytes. I. Time-course changes in fatty acid composition and membrane fluidity during blastic transformation of peripheral blood lymphocytes. Biochim. Biophys. Acta 1044, 323–331.
L. A. Huber, G. B. Xu, G. Jurgens, G. Bock, E. Buhier, K. F. Gey, D. Schonitzer, K. N. Trail, and G. Wick (1991). Correlation of lymphocyte lipid composition, membrane microviscosity and mitogene response in the aged, Eur. J. Immunol 21, 2761–2765.
M. Shinitzky (Eds.) (1984). Physiology of Membrane Fluidity, CRC Press, Boca Raton, FL, pp. 1–52.
M. Shinitzky and M. Inbar (1974). Difference in microviscosity induced by different cholesterol levels in the surface membrane lipid layer of lymphocytes and malignant lymphoma cells. J. Mol. Biol. 85, 603.
L. Klimkane, L. Kalnina, L. Engele, I. Jaunalksne, S. Donina, G. Zakenfelds, and I. Meirovics (1999). in A. Kotyk (Ed.), Third Conference on Fluorescent Microscopy and Fluorescent Probes, Espero Publishing, Prague, pp. 339–345.
I. Kalnina, M. Metra, L. Licitis, and I. Meirovics (1999). in A. Kotyk (Ed.), Third Conference on Fluorescent Microscopy and Fluorescent Probes, Espero Publishing, Prague, pp. 295–300.
R. Bruvere, N. Gabruseva, I. Kalnina, G. Feldmane, and I. Meirovics (2003). Fluorescent characteristics of blood leukocytes of patients with malignant and nonmalignant diseases. J. Fluoresc. 13(2), 149–156.
I. Kalnina, N. Gabruseva, R. Bruvere, T. Zvagule, O. Heislere, A. Volrate, G. Feldmane, and I. Meirovics (2001). Phenotypical characteristics of leukocyte membranes in Chernobyl clean-up workers from Latvia: Use of the fluorescent probe ABM. Proc. Latvian Acad. Sci., Serb. 55(1), 6–13.
T. Namekawa, U. G. V. Vamer, J. J. Goronzy, and C. M. Weyand (1998). Functional subsets of CD-4 T-cells in rheumatoid synovitis. Arthr. Rheumat. 41(12), 2108–2116.
H. Y. Yang, H. Rittner, C. M. Weyand, and J. J. Goronzy, (1999). Aberration in the primarily T-cells receptor repertoire as a predisposition for synovitis inflammation in rheumatoid arthritis. J. Invest. Med. 47(5), 236–245.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kalniņa, I., Zvagule, T., Brū, R. et al. Fluorescent Characteristics of Rheumatoid Arthritis Patients Blood Lymphocytes. J Fluoresc 15, 105–110 (2005). https://doi.org/10.1007/s10895-005-2516-4
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10895-005-2516-4