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RETRACTED ARTICLE: Involvement of a Specific Chemosensory Protein from Bactrocera dorsalis in Perceiving Host Plant Volatiles

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This article was retracted on 01 May 2016

Abstract

Insects have evolved many physiological and behavioral adaptations to recognize external complex chemicals. Olfaction plays an important role in perceiving volatile chemicals, utilizing them to locate host sites, conspecifics, and enemies. Chemosensory proteins (CSPs) are present in high concentrations within the sensory sensilla of insects and are endowed with a heterogeneous range of functions. However, direct evidence for the involvement of CSPs in olfactory function is still lacking. In this study, a fluorescence-based ligand binding assay using Bdor-CSP2 illustrated its ability to bind the majority of the selected ligands of different shapes and chemical structures that are ecologically significant, host plant volatiles of Bactrocera dorsalis. RNAi-mediated silencing coupled with electrophysiological tests showed lower electrophysiological responses to (3Z)-hex-3-en-1-ol, trans-2-hexenal, 6-methylhept-5-en-2-one, and 3-methylbutyl acetate in dsBdor CSP2 treated flies compared with the untreated controls. The reduced expression of Bdor-CSP2 by RNA interference was confirmed by semi-quantitative PCR, real-time quantitative PCR and Western blot, which suggested the RNAi-treatment was responsible for the observed reduction of antennal responses in EAG recordings. These data suggest that the expression of Bdor-CSP2 is necessary for the recognition of antennal responses to some plant host volatiles by B. dorsalis.

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References

  • Anfora G, Vitagliano S, Larsson MC, Witzgall P, Tasin M, Germinara GS, De Cristofaro A (2013) Disruption of Phthorimaea operculella (Lepidoptera: Gelechiidae) oviposition by the application of host plant volatiles. Pest Manag Sci

  • Angeli S, Ceron F, Scaloni A, Monti M, Monteforti G, Minnocci A, Petacchi R, Pelosi P (1999) Purification, structural characterization, cloning and immunocytochemical localization of chemoreception proteins from Schistocerca gregaria. Eur J Biochem 262:745–754

    Article  CAS  PubMed  Google Scholar 

  • Ban L, Zhang L, Yan Y, Pelosi P (2002) Binding properties of a locust’s chemosensory protein. Biochem Biophys Res Commun 293:50–54

    Article  CAS  PubMed  Google Scholar 

  • Biessmann H, Andronopoulou E, Biessmann MR, Douris V, Dimitratos SD, Eliopoulos E, Guerin PM, Iatrou K, Justice RW, Kröber T (2010) The Anopheles gambiae odorant binding protein 1 (AgamOBP1) mediates indole recognition in the antennae of female mosquitoes. PLoS ONE 5:e9471

    Article  PubMed  PubMed Central  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Bruce TJ, Wadhams LJ, Woodcock CM (2005) Insect host location: a volatile situation. Trends Plant Sci 10:269–274

    Article  CAS  PubMed  Google Scholar 

  • Clarke AR, Armstrong KF, Carmichael AE, Milne JR, Raghu S, Roderick GK, Yeates DK (2005) Invasive phytophagous pests arising through a recent tropical evolutionary radiation: the Bactrocera dorsalis complex of fruit flies. Annu Rev Entomol 50:293–319

    Article  CAS  PubMed  Google Scholar 

  • Cui SY, Xu WH (2006) Molecular characterization and functional distribution of N-ethylmaleimide-sensitive factor in Helicoverpa armigera. Peptides 27:1226–1234

    Article  CAS  PubMed  Google Scholar 

  • Dani FR, Michelucci E, Francese S, Mastrobuoni G, Cappellozza S, La Marca G, Niccolini A, Felicioli A, Moneti G, Pelosi P (2011) Odorant-binding proteins and chemosensory proteins in pheromone detection and release in the silkmoth Bombyx mori. Chem Senses 36:335–344

    Article  CAS  PubMed  Google Scholar 

  • Deng S, Yin J, Zhong T, Cao Y, Li K (2012) Function and immunocytochemical localization of two novel odorant-binding proteins in olfactory sensilla of the scarab beetle Holotrichia oblita faldermann (Coleoptera: Scarabaeidae). Chem Senses 37:141–150

    Article  CAS  PubMed  Google Scholar 

  • Große-Wilde E, Svatoš A, Krieger J (2006) A pheromone-binding protein mediates the bombykol-induced activation of a pheromone receptor in vitro. Chem Senses 31:547–555

    Article  PubMed  Google Scholar 

  • Große-Wilde E, Gohl T, Bouché E, Breer H, Krieger J (2007) Candidate pheromone receptors provide the basis for the response of distinct antennal neurons to pheromonal compounds. Eur J Neurosci 25:2364–2373

    Article  PubMed  Google Scholar 

  • Gu SH, Wang SY, Zhang XY, Ji P, Liu JT, Wang GR, Wu KM, Guo YY, Zhou JJ, Zhang YJ (2012) Functional characterizations of chemosensory proteins of the alfalfa plant bug Adelphocoris lineolatus indicate their involvement in host recognition. PLoS ONE 7, e42871

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gu S-H, Zhou JJ, Wang GR, Zhang YJ, Guo YY (2013) Sex pheromone recognition and immunolocalization of three pheromone binding proteins in the black cutworm moth Agrotis ipsilon. Insect Biochem Mol Biol 43:237–251

    Article  CAS  PubMed  Google Scholar 

  • Guo W, Wang X, Ma Z, Xue L, Han J, Yu D, Kang L (2011) CSP and takeout genes modulate the switch between attraction and repulsion during behavioral phase change in the migratory locust. PLoS Genet 7:e1001291

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Immacolata Iovinella FB, Caputo B, della Torre A, Pelosi P (2013) Ligand-binding study of Anopheles gambiae chemosensory proteins. Chem Senses 4:3–11

    Google Scholar 

  • Jayanthi PDK, Woodcock CM, Caulfield J, Birkett MA, Bruce TJ (2012) Isolation and identification of host cues from mango, mangifera indica, that attract gravid female oriental fruit fly, Bactrocera dorsalis. J Chem Ecol 38:361–369

    Article  PubMed  Google Scholar 

  • Leal WS, Chen AM, Ishida Y, Chiang VP, Erickson ML, Morgan TI, Tsuruda JM (2005) Kinetics and molecular properties of pheromone binding and release. Proc Natl Acad Sci U S A 102:5386–5391

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li X, Lu D, Liu X, Zhang Q, Zhou X (2011a) Ultrastructural characterization of olfactory sensilla and immunolocalization of odorant binding and chemosensory proteins from an ectoparasitoid Scleroderma guani (Hymenoptera: Bethylidae). Int J Biol Sci 7:848

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li X, Zhang M, Zhang H (2011b) RNA interference of four genes in adult Bactrocera dorsalis by feeding their dsRNAs. PLoS ONE 6:e17788

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu X, Luo Q, Zhong G, Rizwan-ul-Haq M, Hu M (2010) Molecular characterization and expression pattern of four chemosensory proteins from diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). J Biochem 148:189–200

    Article  CAS  PubMed  Google Scholar 

  • Liu R, He X, Lehane S, Lehane M, Hertz-Fowler C, Berriman M, Field L, Zhou JJ (2012) Expression of chemosensory proteins in the tsetse fly Glossina morsitans morsitans is related to female host‐seeking behaviour. Insect Mol Biol 21:41–48

    Article  PubMed  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  • Maleszka R, Stange G (1997) Molecular cloning, by a novel approach, of a cDNA encoding a putative olfactory protein in the labial palps of the moth Cactoblastis cactorum. Gene 202:39–43

    Article  CAS  PubMed  Google Scholar 

  • Maleszka J, Foret S, Saint R, Maleszka R (2007) RNAi-induced phenotypes suggest a novel role for a chemosensory protein CSP5 in the development of embryonic integument in the honeybee (Apis mellifera). Dev Genes Evol 217:189–196

    Article  CAS  PubMed  Google Scholar 

  • Metcalf RL, Metcalf ER (1992) Plant kairomones in insect ecology and control. Springer

  • Müller C, Hilker M (2000) The effect of a green leaf volatile on host plant finding by larvae of a herbivorous insect. Naturwissenschaften 87:216–219

    Article  PubMed  Google Scholar 

  • Nomura A, Kawasaki K, Kubo T, Natori S (1992) Purification and localization of p10, a novel protein that increases in nymphal regenerating legs of Periplaneta americana (American cockroach). Int J Dev Biol 36:391

    CAS  PubMed  Google Scholar 

  • Pelletier J, Guidolin A, Syed Z, Cornel AJ, Leal WS (2010) Knockdown of a mosquito odorant-binding protein involved in the sensitive detection of oviposition attractants. J Chem Ecol 36:245–248

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pelosi P (1994) Odorant-binding proteins. Crit Rev Biochem Mol Biol 29:199–228

    Article  CAS  PubMed  Google Scholar 

  • Pelosi P, Zhou J-J, Ban L, Calvello M (2006) Soluble proteins in insect chemical communication. Cell Mol Life Sci CMLS 63:1658–1676

    Article  CAS  PubMed  Google Scholar 

  • Qiao HL, Deng PY, Li DD, Chen M, Jiao ZJ, Liu ZC, Zhang YZ, Kan YC (2013) Expression analysis and binding experiments of chemosensory proteins 4 indicate multiple roles in Bombyx mori. J Insect Physiol 59:667–675

    Article  CAS  PubMed  Google Scholar 

  • Stamnes MA, Shieh B-H, Chuman L, Harris GL, Zuker CS (1991) The cyclophilin homolog ninaA is a tissue-specific integral membrane protein required for the proper synthesis of a subset of Drosophila rhodopsins. Cell 65:219–227

    Article  CAS  PubMed  Google Scholar 

  • Sun L, Gu SH, Xiao HJ, Zhou JJ, Guo YY, Liu ZW, Zhang YJ (2013) The preferential binding of a sensory organ specific odorant binding protein of the alfalfa plant bug Adelphocoris lineolatus alinOBP10 to biologically active host plant volatiles. J Chem Ecol 39:1221–1231

    Article  CAS  PubMed  Google Scholar 

  • Vet LE, Dicke M (1992) Ecology of infochemical use by natural enemies in a tritrophic context. Annu Rev Entomol 37:141–172

    Article  Google Scholar 

  • Vieira FG, Rozas J (2011) Comparative genomics of the odorant-binding and chemosensory protein gene families across the Arthropoda: origin and evolutionary history of the chemosensory system. Genome Biol Evol 3:476

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Visser J (1986) Host odor perception in phytophagous insects. Annu Rev Entomol 31:121–144

    Article  Google Scholar 

  • Vogt RG, Riddiford LM, Prestwich GD (1985) Kinetic properties of a sex pheromone-degrading enzyme: the sensillar esterase of Antheraea polyphemus. Proc Natl Acad Sci U S A 82:8827–8831

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Voller A, Bidwell DE, Burek CL, 1980. An enzyme-linked immunosorbent assay (ELISA) for antibodies to thyroglobulin. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, NY). Royal Society of Medicine, pp. 402–405

  • Vosshall LB, Stocker RF (2007) Molecular architecture of smell and taste in Drosophila. Annu Rev Neurosci 30:505–533

    Article  CAS  PubMed  Google Scholar 

  • Yin XW, Iovinella I, Marangoni R, Cattonaro F, Flamini G, Sagona S, Zhang L, Pelosi P, Felicioli A (2013) Odorant-binding proteins and olfactory coding in the solitary bee Osmia cornuta. Cell Mol Life Sci 70:3029–3039

    Article  CAS  PubMed  Google Scholar 

  • Zhang Y, Dong X, Liu J, Hu M, Zhong G, Geng P, Yi X (2012) Molecular cloning, expression and molecular modeling of chemosensory protein from Spodoptera litura and its binding properties with rhodojaponin III. PLoS ONE 7:e47611

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhao N, Guan J, Ferrer JL, Engle N, Chern M, Ronald P, Tschaplinski TJ, Chen F (2010) Biosynthesis and emission of insect-induced methyl salicylate and methyl benzoate from rice. Plant Physiol Biochem 48:279–287

    Article  CAS  PubMed  Google Scholar 

  • Zheng W, Zhu C, Peng T, Zhang H (2012) Odorant receptor co-receptor Orco is upregulated by methyl eugenol in male Bactrocera dorsalis (Diptera: Tephritidae). J Insect Physiol 58:1122–1127

    Article  CAS  PubMed  Google Scholar 

  • Zhou SH, Zhang J, Zhang SG, Zhang L (2008) Expression of chemosensory proteins in hairs on wings of Locusta migratoria (Orthoptera: Acrididae). J Appl Entomol 132:439–450

    Article  Google Scholar 

  • Zhou SH, Zhang SG, Zhang L (2009) The chemosensilla on tarsi of Locusta migratoria (Orthoptera: Acrididae): distribution, ultrastructure, expression of chemosensory proteins. J Morphol 270:1356–1363

    Article  PubMed  Google Scholar 

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Acknowledgments

The work was supported by the National Natural Science Foundation of China for grant no. 31071713, and the China Postdoctoral Science Foundation (2013M542185). We thank Dr XiaoLin Dong for reviewing and providing useful comments regarding this manuscript.

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Authors and Affiliations

  1. Laboratory of Insect Toxicology, Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China

    Xin Yi, PeiDan Wang, Zheng Wang, Jun Cai & MeiYing Hu

  2. Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, PR China, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China

    GuoHua Zhong

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  1. Xin Yi
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Correspondence to GuoHua Zhong.

Additional information

This article has been retracted by Xin Yi, PeiDan Wang, Zheng Wang, Jun Cai, MeiYing Hu, and GuoHua Zhong. Reason: The authors have discovered a serious mistake in the paper. 3-Methylbutyl acetate was reported as binding to the protein. Upon further experimentation, it did not bind. The reason is unclear. After a reevaluation of this work, they no longer have confidence in the finding. Further validation is needed before a firm conclusion can be reached. The authors do not want to further mislead readers of this article.

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Yi, X., Wang, P., Wang, Z. et al. RETRACTED ARTICLE: Involvement of a Specific Chemosensory Protein from Bactrocera dorsalis in Perceiving Host Plant Volatiles. J Chem Ecol 40, 267–275 (2014). https://doi.org/10.1007/s10886-014-0406-4

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  • DOI: https://doi.org/10.1007/s10886-014-0406-4

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